Determination of genotoxic effect of trifluralin on Colossoma macropomum (Teleostei: Characidae: Serrasalminae, Cuvier, 1816) using a multibiomarker approach

Abstract

The herbicide trifluralin is one of the most widely herbicides used in weed control. This study aims to evaluate the genotoxic potential of the trifluralin herbicide on the Colossoma macropomum (tambaqui) using a multi-biomarker approach. The C. macropomum is highly adapted and predominant species in a variety of water bodies in the delta region of the São Francisco River and other river systems in Brazil. The mutagenic and the genotoxic effects of different trifluralin concentrations (0.25, 0.5, 0.75, 1.0 mg L-1) in peripheral erythrocytes of C. macropomum were investigated using micronucleus (MN), comet assay and apoptosis test. After an exposure period of 96 h, the results showed a significant rate of micronuclei and nuclear abnormalities in erythrocytes of C. macropomum exposed to 0.5, 0.75, 1.0 mg L-1 of trifluralin. Compared to controls, the genetic damage index (GDI) showed significant variations (**p < 0.01 and ***p < 0.001) for all the trifluralin concentrations tested. The genotoxicity of trifluralin herbicide on C. macropomum was confirmed by the micronucleus test and comet assay.

Protective Effects of Aloe Vera Extract Administiration against Trifluralin in Drosophila melanogaster with Various Parameters

“…Trifluralin (TRF; 2,6-dinitro-N, N-dipropyl-4trifluoromethylaniline) is a Class 3 carcinogenic herbicide that falls into the class of dinitroaniline. According to the data from EPA (United States Environmental Protection Agency), it is reported that TRF has toxic effects above 0.5 ppm [4] and is genotoxic in various experiments [5,6,7]. TRF has been used in agriculture since 1963.…”

Effects of water quality on aspects of reproductive biology of Cnesterodon decemmaculatus

“…Trifluralin, found at CB at the end of the breeding season, was the most concentrated pesticide quantified (134 ng•L −1 ), exceeding the Canadian Water Quality Guidelines (CCME, 2017) for aquatic biota protection in freshwater systems (0.2 ng•L −1 ). This is a selective preemergence herbicide, which has been classified as highly toxic to fish, it has genotoxic potential, and negatively effects on the pituitary gland function (Könen and Çavaş, 2008;Silva et al, 2017).…”