Determination of genotoxic damages of picloram and dicamba with comet assay in Allium cepa rooted in tissue culture and distilled water

Özel, Çiğdem Alev; Ünal, Fatma; Avuloğlu-Yılmaz, Ece; Erikel, Esra; Mirici, Semra; Yüzbaşıoğlu, Deniz

doi:10.1007/s11033-022-07712-7

Cited by 7 publications

(3 citation statements)

References 40 publications

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“…Both herbicides induced a more severe stress and more pronounced DNA damage in the cells of roots grown under tissue culture conditions. This study confirmed the genotoxic effects of two growth regulators on plant cells [150].…”

Section: Comet Assays In Plant Ecotoxicology and Biological Monitoringsupporting

confidence: 82%

“…All three compounds showed a cytotoxic effect by reducing the root growth and mitotic index, and a genotoxic effect by increasing chromosome aberrations and DNA damage, as compared to control roots [124,125,149]. Furthermore, the genotoxic effects of two herbicides representing synthetic auxins, picloram and dicamba, on root meristems of A. cepa were evaluated utilizing Comet assays in roots grown either under conditions simulating tissue culture (i.e., aseptic conditions using Murashige and Skoog (MS) medium) or in bidistilled water [150]. Both herbicides induced a more severe stress and more pronounced DNA damage in the cells of roots grown under tissue culture conditions.…”

Section: Comet Assays In Plant Ecotoxicology and Biological Monitoringmentioning

confidence: 99%

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Comet Assay: Multifaceted Options for Studies of Plant Stress Response

Tyutereva,

Strizhenok,

Kiseleva

et al. 2024

Horticulturae

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Contrarily to chronic stresses, acute (i.e., fast and dramatic) changes in environmental factors like temperature, radiation, concentration of toxic substances, or pathogen attack often lead to DNA damage. Some of the stress factors are genotoxic, i.e., they damage the DNA via physical interactions or via interference with DNA replication/repair machinery. However, cytotoxic factors, i.e., those that do not directly damage the DNA, can lead to secondary genotoxic effects either via the induction of the production of reactive oxygen, carbon, or nitrogen species, or via the activation of programmed cell death and related endonucleases. The extent of this damage, as well as the ability of the cell to repair it, represent a significant part of plant stress responses. Information about DNA damage is important for physiological studies as it helps to understand the complex adaptive responses of plants and even to predict the outcome of the plant’s exposure to acute stress. Single cell gel electrophoresis (Comet assay) provides a convenient and relatively inexpensive tool to evaluate DNA strand breaks in the different organs of higher plants, as well as in unicellular algae. Comet assays are widely used in ecotoxicology and biomonitoring applications; however, they are still relatively rarely used in physiological studies. In this review, we provide an overview of the basic principles and of useful variations of the protocols of Comet assays, as well as of their use in plant studies, in order to encourage plant physiologists to include this tool in the analysis of plant stress responses.

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Section: Comet Assays In Plant Ecotoxicology and Biological Monitoringsupporting

confidence: 82%

Section: Comet Assays In Plant Ecotoxicology and Biological Monitoringmentioning

confidence: 99%

Comet Assay: Multifaceted Options for Studies of Plant Stress Response

Tyutereva,

Strizhenok,

Kiseleva

et al. 2024

Horticulturae

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“…In previous research, the mutagenic action of ME and IM was demonstrated in P. sativum and Allium cepa [ 36 , 37 ]. In our study, we observed a similar effect of ME and IM on P. sativum seeds.…”

Section: Discussionmentioning

confidence: 99%

Exposure of Pisum sativum L. Seeds to Methomyl and Imidacloprid Cause Genotoxic Effects in Pollen-Mother Cells

Siddiqui

Alrumman

2022

Biology

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Pesticides are commonly used in modern agricultural systems to protect the plants from pests. Even though they potentially increase the crop yield, they have undesirable toxic effects on the consumers of plant products and nontarget host plants. However, there are limited studies to demonstrate the cytological changes induced by pesticides on plant cells. In the present study, we assess the cytological changes induced by two most commonly used insecticides, methomyl (ME) and imidacloprid (IM), using Pisum sativum L. as model plant system. P. sativum seeds were exposed to various concentrations of ME and IM (0.1, 0.2, 0.3, 0.4 and 0.5%) for 1, 3, and 6 h, and their effects on seed germination (SG), radicle length (RL), mitotic index (MI), chromosomal aberrations frequency (CAF), and micronucleus frequency (MNF) were studied. The results indicate that these insecticides decrease MI in root-tip cells, and increase in the MNF in pollen-mother cells in a dose-dependent manner. Additionally, insecticide-treated groups showed a dose- and time-dependent increase in the percentage of aberrant meiotic cells. Clumped nuclei (CNU), stickiness (STC), bridges (BRs), laggards (LGs), secondary association (SA), and precocious separation (PS) were among the frequently observed anomalies. The findings of this study indicate that commonly used insecticides ME and IM have substantial genotoxic effects on the root-tip and pollen-mother cells of P. sativum L.

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A scoping review of recent advances in the application of comet assay to Allium cepa roots

Alias

Zerbini

Feretti

2023

Environ and Mol Mutagen

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The comet assay is a sensitive method for the evaluation of DNA damages and DNA repair capacity at single‐cell level. Allium cepa is a well‐established plant model for toxicological studies. The aim of this scoping review was to investigate the recent application of the comet assay in Allium cepa root cells to assess the genotoxicity. To explore the literature a search was performed selecting articles published between January 2015 and February 2023 from Web of Science, PubMed, and Scopus databases using the combined search terms “Comet assay” and “Allium cepa”. All the original articles that applied the comet assay to Allium cepa root cells were included. Of the 334 records initially found, 79 articles were identified as meeting the inclusion criteria. Some studies reported results for two or more toxicants. In these cases, the data for each toxicant were treated separately. Thus, the number of analyzed toxicants (such as chemicals, new materials, and environmental matrices) was higher than the number of selected papers and reached 90. The current use of the Allium‐comet assay seems to be directed towards two types of approach: the direct study of the genotoxicity of compounds, mainly biocides (20% of analyzed compounds) and nano‐ and microparticles (17%), and assessing a treatment's ability to reduce or eliminate genotoxicity of known genotoxicants (19%). Although the genotoxicity identified by the Allium‐comet assay is only one piece of a larger puzzle, this method could be considered a useful tool for screening the genotoxic potential of compounds released into the environment.

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Determination of genotoxic damages of picloram and dicamba with comet assay in Allium cepa rooted in tissue culture and distilled water

Cited by 7 publications

References 40 publications

Comet Assay: Multifaceted Options for Studies of Plant Stress Response

Comet Assay: Multifaceted Options for Studies of Plant Stress Response

Exposure of Pisum sativum L. Seeds to Methomyl and Imidacloprid Cause Genotoxic Effects in Pollen-Mother Cells

A scoping review of recent advances in the application of comet assay to Allium cepa roots

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