Determination of genetic variance and the genetic dimension of cultivars of the bean genus Phasealus L. using RAPD - PCR indicators

Abstract

The current research aims to determine the DNA Finger Printing and Genetic Distance for 12 cultivars species of the genus Phasealus L Bean genus. Using RAPD-PRC Randomly Amplified Polymorphic DNA PCR indicators, which were extracted for DNA from young leaves of the cultivars, the purity and concentration regarding DNA have been measured with the use of a nanodrop device. Through the results obtained, the used primers were selected (804), including (478) general packages and (326) differentiated packages, and the occurrence of genetic fingerprints for most varieties characterized by some distinct packages amounting to (13) packages, including (8) unique packages and (5) An absent bundle, and the cultivar Brs - Pitanjo got the largest number of unique bundles, it owned (3) bundles. In contrast, the cultivars Brinco, Dellaregina, and massorn have been characterized to have the lowest number of bundles, amounting to (1) bundle. The absent bundles distinguished the two cultivars Brs - Pitanjo, BRs - Executive The highest number reached (2) bundles, while the Frasca variety recorded the lowest number of bundles reached (1) bundle.

Estimation of Genetic Distance Using RAPD Markers for Five Varieties of Citrullus lanatus L.

“…Researchers divided these markers to hybridizationbased DNA and PCR-based DNA markers [4]. Genetic studies played an important role in plant classification by studying the genetic distance and convergence among plant species and identifying the distinctive cultivated varieties [5,6]. Thus, determining the genetic distance in adding and modulating genetic markers serve the goals of plant breeding and improvement.…”