Determination of ganglioside concentrations in breast milk and serum from Malaysian mothers using a high performance liquid chromatography-mass spectrometry-multiple reaction monitoring method

Ma, Lin; MacGibbon, Alastair; Mohamed, Hamid Jan B. Jan; Loy, See-Ling; Rowan, Angela; McJarrow, Paul; Fong, Bertram

doi:10.1016/j.idairyj.2015.05.006

Cited by 50 publications

(91 citation statements)

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“…Separation of the gangliosides was achieved on a Hypersil APS-2 column (150 mm × 2.1 mm, 3 µm; Thermo Scientific, Waltham, MA, USA), using a mobile phase consisting of acetonitrile and ammonium acetate buffer, as described in Ma et al [12]. The eluate from the HPLC system was introduced into a SCIEX QTRAP 6500 mass spectrometer using an electrospray ionisation (ESI) probe inlet.…”

Section: Hplc-ms Quantification Of Gangliosidesmentioning

confidence: 99%

“…The Q1 and Q3 scan widths were set at 0.2 and 0.7 scan, respectively, with a scan range from 700 to 1650 m/z. The detailed HPLC separation and MS operating conditions, together with the method validation data for HBM, are described in Ma et al [12].…”

Section: Hplc-ms Quantification Of Gangliosidesmentioning

confidence: 99%

“…A number of studies have reported ganglioside concentrations in HBM for different population groups, ranging from as low as 1.7 mg l -1 [8] to as high as 23.8 mg l -1 [9]. This variation in concentration has been attributed to differences among the various extraction and analytical methodologies used for measuring these gangliosides, population demographics, diet, time of sampling and study protocol [10][11][12].…”

Section: Introductionmentioning

confidence: 99%

“…All recently published HBM ganglioside data have been generated using high-performance liquid chromatographymass spectrometry (HPLC-MS) techniques [12][13][14][15], given the greater sensitivity and specificity of this technique, whereas earlier data were generated using traditional highperformance thin layer chromatography (HPTLC) methods, which are less sensitive and selective [9,[16][17][18].…”

Section: Introductionmentioning

confidence: 99%

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Lactational changes in concentration and distribution of ganglioside molecular species in human breast milk from Chinese mothers

Liu

MacGibbon

et al. 2015

Lipids

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Gangliosides play a critical role in human brain development and function. Human breast milk (HBM) is an important dietary source of gangliosides for the growing infant. In this study, ganglioside concentrations were measured in the breast milk from a cross-sectional sample of Chinese mothers over an 8-month lactation period. The average total ganglioside concentration increased from 13.1 mg/l during the first month to 20.9 mg/l by 8 months of lactation. The average concentration during the typically solely breast-feeding period of 1‒6 months was 18.9 mg/l. This is the first study to report the relative distribution of the individual ganglioside molecular species through lactation for any population group. The ganglioside molecular species are made up of different fatty acid moieties that influence the physical properties of these gangliosides, and hence affect their function. The GM(3) molecular species containing long-chain acyl fatty acids had the most prominent changes, increasing in both concentration and relative distribution. The equivalent long-chain acyl fatty acid GD(3) molecular species typically decreased in concentration and relative distribution. The lactational trends for both concentration and relative distribution for the very long-chain acyl fatty acid molecular species were more varied. The major GM(3) and GD(3) molecular species during lactation were d40:1 and d42:1, respectively. An understanding of ganglioside molecular species distribution in HBM is essential for accurate application of mass spectrometry methods for ganglioside quantification.

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Section: Hplc-ms Quantification Of Gangliosidesmentioning

confidence: 99%

Section: Hplc-ms Quantification Of Gangliosidesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Lactational changes in concentration and distribution of ganglioside molecular species in human breast milk from Chinese mothers

Liu

MacGibbon

et al. 2015

Lipids

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“…Foods of plant origin are not a source of gangliosides as they lack the pathway to synthesize sialic acid [7]. Estimates of ganglioside contents in human milk ranged between 1.7 and 68.6 mg/L [17][18][19][20], while that for reconstituted infant formula is about 2-16 mg/L [20][21][22][23]. Infants receiving bovine-based milk formula are reported to ingest as little as 20% of the ganglioside consumed by exclusively breastfed infants [22].…”

Section: Introductionmentioning

confidence: 99%

Correlation between dietary intake and serum ganglioside concentrations: a cross-sectional study among Malaysian toddlers

et al. 2016

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Background: Gangliosides are a group of sialylglycosphingolipids, widely distributed in body tissues, mainly as components of plasma membranes. They play crucial roles in neurodevelopment, gut maturation, and immune system. Dietary gangliosides have been shown to bring about benefits including cognition and immune support for breastfed infants. There is dearth of studies on dietary gangliosides intake or plasma ganglioside levels for toddlers. Given toddlers are still growing rapidly, a good understanding of ganglioside intake during this early childhood period is important for future dietary recommendations. The aim of this study was to provide information on dietary ganglioside intake in Malaysian toddlers and correlations with serum ganglioside levels. Methods: Toddlers who fulfilled the inclusion criteria were recruited from the Federal Territory of Putrajaya and neighboring urban suburbs. Background characteristics and food intake using food frequency questionnaire were collected for the entire sample (n = 153). As for ganglioside correlation determination, a 2 day weighed food record was conducted on a sub-group who provided blood (n = 74). Ganglioside levels in the food and blood were determined using modern high performance liquid chromatography mass spectrometry method. Results: The average dietary intake of total gangliosides for the Malaysian toddlers (aged 12-24.5 months) was 5.86 ± 0.56 mg/day. Growing up milks had a wide ganglioside concentration range (0.03 11.4 mg/100 g), and were the major contributor to dietary ganglioside intake (85%). The remaining dietary gangliosides were provided by other dairy products, meat, fish, bakery and biscuits. Serum levels varied from 5.05 μg/mL to 16.15 μg/mL. While no significant correlation was observed between dietary ganglioside intake from growing up milks and serum ganglioside levels in the toddlers, there was a significant but weak correlation between dietary ganglioside intake from dairy products (r = 0.241; p = 0.038) and meat (r = 0.294; p = 0.010) with serum ganglioside levels Conclusions: Gangliosides are a component of the Malaysian toddlers' diet (5.68 ± 0.56 mg/day), and were measured in their plasma at levels ranging from 5.05 to 16.15 μg/mL. Growing up milk contributed to 85% of the total dietary gangliosides intake, with remaining contributions from chicken meat and fish. More studies should be undertaken on the contributions of dietary gangliosides, including breast milk, in bringing about health benefits to young children.

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Lipids in Infant Formula

Zou

Zhang²,

Pedersen

et al. 2020

Bailey's Industrial Oil and Fat Products

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Human milk (HM) provides the ideal nourishment for infant growth and cognitive development. The new advances in component and microstructure analyses of HM provide solid knowledge for developing better infant formulas to mimic HM to the largest extent possible. In this article, we reviewed the latest progress in the analysis and understanding of the physical structures, composition, and phospholipids and other minor components in HM lipids, as well as in its counterpart infant formula (IF) lipids. The similarities and differences of lipids between HM and IF suggest the gaps to be narrowed for improved IF development, as the theme of IF lipids studies, which are not only on fatty acid composition, but also on triacylglycerol (TAG) profiles and minor lipid species and level of supplements. The efforts and researches in searching alternative oils and fats and development of related chemical/enzymatic processes comprise a key focus of this article. The specifications to regulate IF market, marketing trend, and patenting status are also intensively discussed. Moreover, the risk substance controls of 3‐monochloro‐1,2‐propanediol (3‐MCPD esters) and glycidyl esters (GE) and the strategy to mitigate them during IF lipid process are also reviewed.

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Determination of ganglioside concentrations in breast milk and serum from Malaysian mothers using a high performance liquid chromatography-mass spectrometry-multiple reaction monitoring method

Cited by 50 publications

References 39 publications

Lactational changes in concentration and distribution of ganglioside molecular species in human breast milk from Chinese mothers

Lactational changes in concentration and distribution of ganglioside molecular species in human breast milk from Chinese mothers

Correlation between dietary intake and serum ganglioside concentrations: a cross-sectional study among Malaysian toddlers

Lipids in Infant Formula

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