Determination of gabapentin in plasma and urine by high-performance liquid chromatography and pre-column labelling for ultraviolet detection

3-Substituted GABA analogs with central nervous system activity: A review

“…Concentrations of Gabapentin in plasma and urine can be measured by high performance liquid chromatography (HPLC) 38 after derivatization, utilizing UV detection. Gas chromatography can also be used after derivatization.…”

“…Compound 36 was also found to increase latency to seizure at this dose level. Bridged bicyclic compounds have also been prepared, including (9-Aminomethyl-bicyclo[3.3.1]non-9-yl)-acetic acid hydrochloride (37) and (7-Aminomethyl-bicyclo[2.2.1]hept-7-yl)-acetic acid hydrochloride (38). 86,90 Both of these compounds had similar affinity to 36 at the gabapentin-binding site.…”

Validated HPLC method for the determination of gabapentin in human plasma using pre-column derivatization with 1-fluoro-2,4-dinitrobenzene and its application to a pharmacokinetic study

“…Gabapentin has no significant ultraviolet, visible or fluorescence absorption. Several HPLC methods have been published for determination of gabapentin in human plasma using different derivatizing reagents such as 2,4,6-trinitrobenzene sulphonic acid [5,6], phenylisothiocyanate (PITC) [7], O-phthaldialdehyde (OPA) [8][9][10][11][12][13][14], 4-chloro-7-nitrobenzofurazan [15], 9-fluorenylmethylchloroformate [16] and 1, 2-naphthoquinone-4-sulphonic acid sodium salt [17].…”

Optimization of an HPLC Method for Determination of Gabapentin in Dosage Forms through Derivatization with 1-Fluoro-2,4-dinitrobenzene

“…Derivatization by chromophoric reagents increases the sensitivity of gabapentin detection. Several HPLC methods for determination of gabapentin in human plasma have been published using different derivatizing reagents such as 2,4,6-trinitrobenzenesulphonic acid, 3,4) phenylisothiocyanate, 5) o-phthaldialdehyde (OPA), [6][7][8][9][10][11][12] 4-chloro-7-nitrobenzofurazan, 13) 9-fluorenylmethyl chloroformate 14) and 1,2-naphthoquinone-4-sulphonic acid sodium salt. 15) Most of the proposed methods could be optimized for determination of gabapentin in dosage forms but they suffer from some limitations such as a lengthy run time or using special reaction conditions which may not be suitable for routine analysis.…”