Determination of fructose 2,6-bisphosphate in human lymphocytes is a potential laboratory test in diabetology

Belyaeva, N. F.; Голубев, М. А.; Markova, Mariya; Kol'chenko, O. L.; Lamzina, N. N.; Gorodetskiĭ, V K; Viktorova, L. N.; Балаболкин, М. И.; Korovkin, B. F.

doi:10.1007/bf02446591

Cited by 2 publications

(1 citation statement)

References 9 publications

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“…In hepatocyte, diabetes mellitus enhances phosphorylation of fructose-2,6-bisphosphate leading to a decrease in the activity of the enzyme causing hyperglycemia. In peripheral blood lymphocytes fructose-2,6-bisphosphate system is slightly different from that of hepatocyte 4 . The activation of glycolysis by fructose-2,6-bisphosphate in peripheral blood mononuclear cells causes the accumulation of glycolytic metabolites and inhibits the activation of immune cells 5 .These altered metabolic products and oxidative stress play a role in the development of dia-betic complications 6 .…”

Section: Introductionmentioning

confidence: 81%

Levels of Fructose-2,6-Bisphosphate in Lymphocytes of Diabetic Patients.

Seemen Ghafoor,

Azra Saleem,

Abdullah Abbasi

et al. 2013

j Muhammad Med Coll

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Background: Patients with diabetes mellitus have infections more often than those without diabetes. Severalfactors predispose diabetic patients to infections, including an alteration in immune defense mechanism. Elevated levels of fructose-2,6- bisphosphate in lymphocyte have been shown in diabetic patients. The activation ofglycolysis by fructose -2,6- bisphosphate in peripheral blood mononuclear cells causes the accumulation of glycolytic metabolites and inhibits the activation of immune cells.Objective: To observe and compare the levels of fructose-2,6-bisphosphate in lymphocytes of diabetics and normal subjectsMaterial and Methods: 200 diabetic and 50 control subjects were selected for study .The subjects were evaluated for severity of diabetes and their fasting blood glucose, HbA1C, total leucocyte count, lymphocyte count andfructose-2,6-bisphosphate in lymphocytes were estimated.Results: The results show that mean fasting blood glucose, HbA1C, total leucocytes count and fructose-2,6-bisphosphate levels in lymphocytes were significantly higher (P<0.001) while lymphocyte count was significantlylower (P<0.001) in diabetic patients as compared to control group.Conclusions: It was concluded from the facts observed in this study that elevated levels of fructose-2,6-bisphosphate in lymphocytes and decreased number of lymphocytes may have induced chances of infections indiabetic patients.Keywords: Lymphocytes, Fructose-2,6-bisphosphate, Diabetes, Infections.

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Section: Introductionmentioning

confidence: 81%

Levels of Fructose-2,6-Bisphosphate in Lymphocytes of Diabetic Patients.

Seemen Ghafoor,

Azra Saleem,

Abdullah Abbasi

et al. 2013

j Muhammad Med Coll

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Resonance light scattering detection of fructose bisphosphates using uranyl-salophen complex-modified gold nanoparticles as optical probe

Liao

et al. 2015

Anal Bioanal Chem

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In this paper, we report a resonance light scattering (RLS) method for the determination of fructose bisphosphates (FBPs) in water solution using fructose 1,6-bisphosphate (F-1,6-BP) as a model analyte without the procedure of extracting target analyte. The method used a type of modified gold nanoparticles (GNPs) as optical probe. The modified GNPs are uranyl-salophen-cysteamine-GNPs (U-Sal-Cy-GNPs) which are obtained through the acylation reaction of carboxylated salophen with cysteamine-capped GNPs (Cy-GNPs) to form Sal-Cy-GNPs and then the chelation reaction of uranyl with tetradentate ligand salophen in the Sal-Cy-GNPs. A FBP molecule is used easily to connect two U-Sal-Cy-GNPs to cause the aggregation of the GNPs by utilizing the specific affinity of uranyl-salophen complex to phosphate group, resulting in the production of strong RLS signal from the system. The amount of FBPs can be determined through detecting the RLS intensity change of the system. A linear range was found to be 2.5 to 75 nmol/L with a detection limit of 0.91 nmol/L under optimal conditions. The method has been successfully used to determine FBPs in real samples with the recoveries of 96.5-103.5 %.

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Determination of fructose 2,6-bisphosphate in human lymphocytes is a potential laboratory test in diabetology

Cited by 2 publications

References 9 publications

Levels of Fructose-2,6-Bisphosphate in Lymphocytes of Diabetic Patients.

Levels of Fructose-2,6-Bisphosphate in Lymphocytes of Diabetic Patients.

Resonance light scattering detection of fructose bisphosphates using uranyl-salophen complex-modified gold nanoparticles as optical probe

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