Determination of free radical reaction products and metabolites of salicylic acid using capillary electrophoresis and micellar electrokinetic chromatography

Coolen, Saj Stefan; Huf, FA Fred

doi:10.1016/s0378-4347(98)00289-8

Cited by 36 publications

(22 citation statements)

References 32 publications

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“…However, the analytical procedures are often quite tedious and time consuming. For research purposes, ROS have been determined by direct measurement of free radicals using a chemiluminescence approach [6], by monitoring the hydroxylation of salicylic acid with HPLC or CE [7], and by the use of intracellular redox-sensitive fluorogenic probes [8,9].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of glutathione and reactive oxygen species in individual cells by microchip electrophoresis

2005

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A microchip electrophoresis method was developed for simultaneous determination of reactive oxygen species (ROS) and reduced glutathione (GSH) in the individual erythrocyte cell. In this method, cell sampling, single-cell loading, docking, lysing, and capillary electrophoretic separation with LIF detection were integrated on a microfluidic chip with crossed channels. ROS was labeled with dihydrorhodamine 123 in the intact cell, while GSH was on-chip labeled with 2,3-naphthalene-dicarboxaldehyde, which was included in the separation medium. On-chip electrical lysis, characterized by extremely fast disruption of the cellular membrane (<40 ms), was exploited to minimize enzymatic effects on analyte concentrations during the determination. The microfluidic network was optimized to prevent cell leaking from the sample reservoir (S) into separation during the separation phase. The structure of the S was modified to avoid blockage of its outlet by deposited cells. Detection limits of 0.5 and 6.9 amol for ROS and GSH, respectively, were achieved. The average cell throughput was 25 cells/h. The effectiveness of the method was demonstrated in the simultaneous determination of GSH and ROS in individual cells and the variations of cellular GSH and ROS contents in response to external stimuli.

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Section: Introductionmentioning

confidence: 99%

Simultaneous determination of glutathione and reactive oxygen species in individual cells by microchip electrophoresis

2005

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“…It reacts with ·OH to generate 2,3-DHBA, 2,5-DHBA, and catechol, with a reaction rate constant of 5×10 9 -10 10 L/mol/s; 2,3-DHBA and 2,5-DHBA typically comprise 90% of the total products. Thus, the sum concentration of 2,3-DHBA and 2,5-DHBA following the reaction was assumed to represent the total ·OH concentration in the initial solution [21,22] . CE (HP 3D , Agilent, Germany) was used to measure the SA and DHBAs concentrations, as described by Li et al [18] .…”

Section: Electron Spin Resonance (Esr) Measurementsmentioning

confidence: 99%

Size-dependent hydroxyl radicals generation induced by SiO2 ultra-fine particles: The role of surface iron

Shang

Zhu

et al. 2009

Sci. China Ser. B-Chem.

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Oxidative stress and hydroxyl radicals (·OH) play important roles in adverse health effects caused by inhalable ambient air particles (PM 10 ). The ultra-fine fraction of PM 10 has been hypothesized as one of the critical contributors to ·OH generation. Both in vivo and in vitro studies have shown that ultra-fine particles (UFPs) or nano-particles generate more ·OH than larger particles with identical mass and composition. Both the surface area and surface-adsorbed redox-active metals have been suggested as factors to determine the oxidative capacity of UFPs. In this study, the ·OH-generating capability of different sizes of SiO 2 particles was investigated in order to determine which factor influences particle-induced ·OH generation. The amount of ·OH generated in both acellular and cellular systems was quantified using a capillary electrophoresis method following exposure to SiO 2 particles with diameters of 14, 100, and 500 nm. The amount of ·OH was strongly dependent on particle size, and a significant enhancement was observed only with 14 nm particles. Further studies indicated a close association between ·OH and iron ion concentration (R 2 = 0.812, p＜0.01). Washed particles, with their surface iron being removed, did not generate ·OH. The iron-containing leachate from these washings was able to enhance ·OH production as untreated particles did. Therefore, the presence of adsorbed iron on the surface of the SiO 2 particles is presented as a possible mechanism of UPFs-induced ·OH generation. The SiO 2 acted as an inert substrate, and the surface of ultra-fine SiO 2 particles acted as a carrier for iron.

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“…Generally, cellular redox state is balanced by the relative quantities of the intracellular oxidative (reactive oxygen species, ROS) and reductive substances (reduced glutathione, GSH). Conventionally, intracellular ROS and GSH can only be determined by flow cytometry, HPLC, or enzymatic methods, respectively [27][28][29]. Qin et al [30] described a microfluidic device method with LIF detection for simultaneous and rapid determination of intracellular ROS and GSH in apoptotic leukemia cells on a glass chip.…”

Section: Shift In Cellular Redox Statementioning

confidence: 99%

Microfluidic devices for the analysis of apoptosis

Qin

Liu

et al. 2005

Electrophoresis

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Microfluidic devices for the analysis of apoptosisApoptosis is the outcome of a metabolic cascade that results in cell death in a controlled manner. Due to its important role in maintaining balance in organisms, in mechanisms of diseases, and tissue homeostasis, apoptosis is of great interest in the emerging fields of systems biology. Research into cell death regulation and efforts to model apoptosis processes have become powerful drivers for new technologies to acquire ever more comprehensive information from cells and cell populations. The microfluidic technology promises to integrate and miniaturize many bioanalytical processes, which offers an alternative platform for the analysis of apoptosis. This review aims to highlight the recent developments of microfluidic devices in measuring the hallmarks as well as the dynamic process of cellular apoptosis. The potential capability and an outlook of microfluidic devices for the study of apoptosis are addressed.

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Determination of free radical reaction products and metabolites of salicylic acid using capillary electrophoresis and micellar electrokinetic chromatography

Cited by 36 publications

References 32 publications

Simultaneous determination of glutathione and reactive oxygen species in individual cells by microchip electrophoresis

Simultaneous determination of glutathione and reactive oxygen species in individual cells by microchip electrophoresis

Size-dependent hydroxyl radicals generation induced by SiO2 ultra-fine particles: The role of surface iron

Microfluidic devices for the analysis of apoptosis

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