Determination of free and total polyamines in human serum and urine by ion-pairing high-performance liquid chromatography using a radial compression module

Brossat, Béatrice; Straczek, J.; Belleville, F.; Nabet, P; Metz, R

doi:10.1016/s0378-4347(00)84826-4

Cited by 32 publications

(6 citation statements)

References 18 publications

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“…Whether the scavenging activity of polyamines has any significant anti-inflammatory effect in vivo requires further investigation. The plasma levels of endogenous polyamines are very low -normal human serum [10] gives values for putrescine as 155 +_ 122 spermine, 17 _+ 16, and spermidine 45 +_ 35 n moles/L. Clearly these values are far lower than the ones used in our experiments.…”

Section: Discussioncontrasting

confidence: 59%

In vitro interactions between endogenous polyamines and superoxide anion

1986

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Endogenous polyamines with anti-inflammatory activity scavenge superoxide and possibly other oxy-radicals produced by xanthine oxidase or from stimulated polymorphonuclear leucocytes. Polyamines incubated with stimulated cells are in part metabolised. Putrescine is converted to metabolites tentatively identified as gamma-aminobutyraldehyde, delta'-pyrolline and gamma-aminobutyric acid. The metabolism of spermidine, spermine and cadaverine was not as extensively studied but metabolites were formed that gave positive reaction to Schiffs reagent on tlc plates. The possible relevance of the results to the anti-inflammatory action of polyamines is discussed.

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Section: Discussioncontrasting

confidence: 59%

In vitro interactions between endogenous polyamines and superoxide anion

1986

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“…The concentrations of colistin in plasma, urine, and sputum were determined by high-performance liquid chromatography using an assay developed in our laboratory based on polyamine methods. 16,17 Briefly, a Varian 5060 high-pressure liquid chromatograph and 9090 autosampler (Sugarland, TX) using a Valco C6U switching valve equipped with a 50 µl loop was used for all analyses. Peaks were detected on a Varian 9070 fluorescence detector set at 350 nm excitation, 500 nm emission, and 20 fluorescence units full scale.…”

Section: Determination Of Colistin In Biologic Fluidsmentioning

confidence: 99%

The Pharmacokinetics of Colistin in Patients with Cystic Fibrosis

Reed

Stern

O’Riordan

et al. 2001

The Journal of Clinical Pharma

142

107

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The safety and pharmacokinetics of colistin were determined after first dose (n = 30) and again under steady-state conditions (n = 27) in 31 patients with cystic fibrosis receiving the drug as a component of their treatment for an acute pulmonary exacerbation of their disease. Patients ranged in age from 14 to 53 years and received colistin for 6 to 35 days. Each patient was started on colistin 5 to 7 mg/kg/day administered intravenously in three equally divided doses. Elimination half-life (t1/2), mean residence time (MRT), steady-state volume of distribution (Vdss), total body clearance (Cl), and renal clearance (Clr) after first-dose administration averaged 3.4 hours, 4.4 hours, 0.09 l/kg, and 0.35 and 0.24 ml/min/kg, respectively. No differences in colistin disposition characteristics between first-dose and steady-state evaluations were observed. Sputum sampling was incomplete and confounded by previous aerosol administration but revealed colistin concentrations that markedly exceeded observed plasma concentrations. Twenty-one patients experienced one or more side effects attributed to colistin administration. The most common reactions involved reversible neurologic manifestations, including oral and perioral paresthesias (n = 16), headache (n = 5), and lower limb weakness (n = 5). All of these apparent colistin-induced neurologic adverse effects, though bothersome, were benign and reversible. Intermittent proteinuria was observed on urinalysis in 14 patients, and 1 patient developed reversible, colistin-induced nephrotoxicity. No relationship between the occurrence of any colistin-associated adverse effect and plasma colistin concentration or colistin pharmacokinetic parameter estimate was observed. These data provide no basis for routine monitoring of colistin plasma concentrations to guide dosing for patient safety and suggest slow upward dose titration to minimize the incidence and severity of associated side effects.

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“…system consisting of two 510 pumps, an U6K injector, a radial compression system Z-module equipped with a Radialpak C8 (10 /sm) column (internal diameter 8 mm), a 720 system controller, a 420 fluorescence detector (excitation 360 nm; emission 510 nm) and a 730 data module. The mobile phase consisted of (A) methanol and (B) 0.25 M-triethylammonium phosphate buffer, pH 3.5 (Brossat et al, 1983). Polyamines and y-aminobutyric acid were separated by a linear gradient from 40 to 90% of A over 23 min at a flow rate of 3 ml/min.…”

Section: Incorporation Of 13hlornithine and 13hlputrescinementioning

confidence: 99%

Localization and biosynthesis of polyamines in insulin-producing cells

Hougaard¹,

Nielsen²,

Larsson³

1986

Biochemical Journal

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Two recently developed fluorescence cytochemical methods, specific for spermidine and spermine, were used to localize polyamines in the endocrine pancreas. The polyamines were restricted to the insulin-producing beta-cells and were mainly associated with the secretory granules. Chemical polyamine determinations carried out on isolated rat and mouse pancreatic islets revealed large amounts of polyamines. Compared with extracts of whole pancreas, the islets contained very high concentrations of spermine relative to spermidine. Biosynthesis of polyamines from [3H]ornithine or from [3H]putrescine in isolated islets was significantly stimulated at high glucose concentrations. Moreover, significant incorporation of label from [3H]putrescine was also detected in gamma-aminobutyric acid. This incorporation, however, was not stimulated by high glucose. Possible roles for polyamines associated with the secretory granules in insulin-producing cells are discussed.

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Determination of free and total polyamines in human serum and urine by ion-pairing high-performance liquid chromatography using a radial compression module

Cited by 32 publications

References 18 publications

In vitro interactions between endogenous polyamines and superoxide anion

In vitro interactions between endogenous polyamines and superoxide anion

The Pharmacokinetics of Colistin in Patients with Cystic Fibrosis

Localization and biosynthesis of polyamines in insulin-producing cells

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