Determination of four main components of gentamicin in animal tissues after solid‐phase extraction by high‐performance liquid chromatography/tandem mass spectrometry

Zhang, Congcong; Jian, Hu; Sun, Fang; Jia, Man; Wu, Cuiling; Zheng, Lirong

doi:10.1002/rcm.8234

Cited by 11 publications

(13 citation statements)

References 23 publications

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“…A strong signal suppression of α‐amanitin occurred with the signal reducing to 19.7%–22.2%. Solid‐phase extraction was an alternative solution to reduce the matrix effect, but it was highly variable and highly costly in comparison with protein precipitation 19–21 . According to our previous published research, sample dilution has been proved to be an effective solution to eliminate the strong matrix effect of cycloserine (an analogue of the amino acid) 16 .…”

Section: Resultsmentioning

confidence: 99%

Development of a simple and reliable method for α‐amanitin detection in rat plasma and its application to a toxicokinetic study

Mao

Sun

et al. 2021

Rapid Comm Mass Spectrometry

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Rationale α‐Amanitin is a highly toxic peptide widely found in species of poisonous mushrooms. The matrix effect has been a major obstacle for accurate determination of α‐amanitin in plasma samples by liquid chromatography/tandem mass spectrometry (LC/MS/MS). In this study, the strategy to eliminate the matrix effect of α‐amanitin with a one‐step dilution approach after deproteinization was applied. Methods Rat plasma samples were processed by protein precipitation with methanol followed by a nine‐fold dilution with pure water. The matrix effect value of α‐amanitin was 19.7%–22.2% by protein precipitation and then changed to 87.5%–88.7% after dilution. α‐Amanitin and the internal standard (roxithromycin) were analyzed on an ACQUITY UPLC® BEH C18 (50 mm × 2.1 mm, 1.7 μm) column within 3.0 min by gradient elution. Results The linear ranges were 0.90–600 ng/mL with a correlation coefficient r >0.9958. A lower limit of quantification (LLOQ) of 0.90 ng/mL was achieved using only 50 μL of rat plasma. The intra‐ and inter‐day precisions for the analyte ranged from 3.2% to 7.5% and 3.1% to 7.1%, respectively, and the accuracy ranged from −5.3% to −8.0%. Conclusions The matrix effect of α‐amanitin was reduced by sample dilution after plasma deproteinization. A reliable LC/MS/MS method for the determination of α‐amanitin in rat plasma was developed. This method was successfully applied for a toxicokinetic study of rats after intravenous injection of α‐amanitin with a subacute toxicity dose at 0.10 mg/kg.

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Section: Resultsmentioning

confidence: 99%

Development of a simple and reliable method for α‐amanitin detection in rat plasma and its application to a toxicokinetic study

Mao

Sun

et al. 2021

Rapid Comm Mass Spectrometry

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“…For multi‐class analysis, ACN has been used to extract some antibiotics from different meat tissues (Saito‐Shida, Sakai, Nemoto, & Akiyama, 2017; Tajabadi, Ghambarian, Yamini, & Yazdanfar, 2016) sometimes combined with water (Casey, Andersen, Williams, Nickel, & Ayres, 2020; Lehotay & Lightfield, 2018; Moretti et al., 2016; Song et al., 2017; Yin et al., 2016; Y. Zhang et al., 2018), EDTA (Dasenaki, Michali, & Thomaidis, 2016; Moretti et al., 2016; Yin et al., 2016; Zhao, Lucas, Long, Richter, & Stevens, 2018; Zheng et al., 2019), aqueous ammonium solutions (Dasenaki et al., 2016; Kang et al., 2017), TCA (Dasenaki et al., 2016; Rizzetti, de Souza, Prestes, Adaime, & Zanella, 2017), formic acid (He, Song, Zhou, & Zhao, 2017; Pugajeva, Ikkere, Judjallo, & Bartkevics, 2019; L. Zhao et al., 2018), dimethyl sulfoxide (Zhao et al., 2018), acetic acid (M. Zhang et al., 2018), or ethyl acetate (Yoshikawa et al., 2017). In addition, other solvents such as MeOH with formic acid (Danezis, Anagnostopoulos, Liapis, & Koupparis, 2016) or EDTA (Arslanbaş, 2018), PBS (Oyedeji, Msagati, Williams, & Benson, 2020) with TCA (Sarker et al., 2018), or McIlvaine buffer (Zhang et al., 2016) have been used.…”

Section: Analytical Methodologies For the Analysis Of The Principal Amentioning

confidence: 99%

“…This rapid and easy method provided good LODs (0.2 to 3.0 µg kg –1 ) and recoveries in the range of 67% to 102%. The three approaches (Danezis et al., 2016; Rizzetti et al., 2017; M. Zhang et al., 2018) have in common the use of an organic solvent combined with an acid to increase protein precipitation.…”

Section: Analytical Methodologies For the Analysis Of The Principal Amentioning

confidence: 99%

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Determination of antibiotics in meat samples using analytical methodologies: A review

Moga

Vergara-Barberán

Lerma‐García

et al. 2021

Comp Rev Food Sci Food Safe

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Antibiotics are widely used to prevent or treat some diseases in human and veterinary medicine and also as animal growth promoters. The presence of these compounds in foods derived from food‐producing animals can be a risk for human health. Consequently, regulatory agencies have set maximum residue limits for antibiotics in food samples. Therefore, the development of novel methodologies for its determination in food samples is required. Specifically, the analysis and quantification of these substances in meat tissues is a challenge for the analytical chemistry research community. This is due to the complexity of the matrix and the low detection limits required by the regulatory agencies. In this sense, a comprehensive review on the development of new sample preparation treatments involving extraction, cleanup, and enrichment steps of antibiotics in meat samples in combination with sensitive and sophisticated determination techniques that have been carry out in the last years is necessary. Therefore, the aim of this work is to summarize the published methodologies for the determination of antibiotics from 2016 until the beginning of the second semester of 2020. The first part of this review includes an introduction about antibiotic families, followed by sample preparation and determination techniques applied to the different families. Finally, a detailed discussion of the current trends and the future possible perspectives in this field are also included.

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“…They act to inhibit protein synthesis [42]. GM was determined alone or in combinations by variety of methods as electrochemical methods [43][44][45][46][47], spectrophotometry [48][49][50][51], HPLC [52][53][54][55][56][57][58][59], and LC [60][61][62][63][64][65][66][67]. There is no reported method for the determination of the three drugs together either in their ternary mixture or in the presence of their degradation products.…”

Section: Introductionmentioning

confidence: 99%

Application of TLC-Spectrodensitometric and Chemometric Methods for Determination of Momenta® Cream: A Comparative Study Applied on Ternary Mixture

Salem¹,

MA²,

SM³

et al. 2021

austinjanalpharmchem

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A comparative study for the validation and advancement of two analytical approaches applied for the simultaneous determination of Mometasone Furoate (MF), Miconazole Nitrate (MIC) and Gentamicin (GM) formulated in Momenta® cream. The first approach was TLC-spectrodensitometric method, which was advanced by separating the three components on TLC aluminum plates coated with silica gel 60 F254 using chloroform: methanol: formic acid (4:0.3:0.15, v/v/v) as a mobile phase, then scanned at 254nm using Camage TLC scanner 3 operated in reflectance-absorbance mode. The second approach was the chemometric method using two models: Partial Least Squares (PLS) and Principle Component Regression Model (PCR). The proposed approaches were validated according to ICH guidelines and were applied for the determination of the ternary mixtures in their analytical mixtures and pharmaceutical preparation.

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Determination of four main components of gentamicin in animal tissues after solid‐phase extraction by high‐performance liquid chromatography/tandem mass spectrometry

Cited by 11 publications

References 23 publications

Development of a simple and reliable method for α‐amanitin detection in rat plasma and its application to a toxicokinetic study

Development of a simple and reliable method for α‐amanitin detection in rat plasma and its application to a toxicokinetic study

Determination of antibiotics in meat samples using analytical methodologies: A review

Application of TLC-Spectrodensitometric and Chemometric Methods for Determination of Momenta® Cream: A Comparative Study Applied on Ternary Mixture

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