Determination of folic acid in tablets by microemulsion electrokinetic chromatography

Abstract

A microemulsion electrokinetic chromatography (MEEKC) method has been developed and validated for the determination of folic acid, a water-soluble vitamin, in a commercial tablet formulation. The analysis was performed using a microemulsion containing 0.5% (w/w) ethyl acetate, 1.2% (w/w) butan-1-ol, 0.6% (w/w) sodium dodecyl sulfate, 15% (v/v) 2-propanol and 82.7% (w/w) 10 mmol L(-1) sodium tetraborate aqueous buffer at pH 9.2. Direct UV detection at 214nm led to an adequate sensitivity without interference from sample excipients. For quantitative purposes, niacin was used as internal standard. Acceptable precision (<1.2% relative standard deviation (R.S.D.)), linearity (r = 0.9992; range from 160.0 to 240.0 microg/mL), sensitivity (limit of detection (LOD) = 2.98 microg/mL; limit of quantification (LOQ) = 9.05 microg/mL) and recovery (99.8 +/- 1.8% at three concentration levels) were obtained. Based on the performance characteristics, the proposed methodology was found suitable for the determination of folic acid in tablet formulations.

Folic acid Determination in neutral pH electrolyte by Adsorptive Stripping Voltammetry at the Mercury Film Electrode

“…The terms folic acid and folates are often used interchangeably, but folic acid is approximately twice as bioavailable as the folates [3]. Analytical methods for folic acid include bioassays [4], enzymatic methods [5], fluorimetry [6][7][8][9][10][11][12][13], spectrophotometry [14][15][16], chromatography [17][18][19][20][21][22][23][24][25], chemiluminescence [26][27][28], phosphorimetry [29] and inductively coupled plasmaatomic emission spectroscopy [30]. Electrochemical methods have been used to detect folic acid [31][32][33][34][35][36][37][38][39][40][41][42] and to investigate its redox properties [43].…”

Comparison of oil‐in‐water and water‐in‐oil microemulsion electrokinetic chromatography as methods for the analysis of eight phenolic acids and five diterpenoids

“…Many reports on O/W MEEKC concluded that variations in the nature and concentration of oils, organic modifier and surfactant had a noticeable effect on separation [38][39][40]. Similarly, the above three factors were also utilized to improve MEEKC separations.…”

Capillary electrophoresis for the analysis of small‐molecule pharmaceuticals

“…The accuracy of the method (% recovery) was found to be 101-106% for ragaglitazar and 101-125% for arginine, while precision for the detection of peaks (% RSD) was found to be 0.63% for ragaglitazar and 3.50% for arginine. An MEEKC method was used for the quantitative determination of folic acid in tablets [137] giving a precision of ,1.2% RSD and recovery of 99.8 6 1.8% at three concentration levels.…”