Determination of Flurazepam and Its Major Biotransformation Products in Blood and Urine by Spectrophotofluorometry and Spectrophotometry

Silva, J. Arthur F. de; Strojny, Norman

doi:10.1002/jps.2600600903

Cited by 72 publications

(6 citation statements)

References 7 publications

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“…Studies on the biotransformation and elimination of flurazepam in humans have shown that the compound is extensively metabolized and has a large apparent volume of distribution (3). Peak plasma concentrations of the intact drug rarely exceed several nanograms per milliter following the single or multiple dose administration of therapeutic amounts of flurazepam to humans (4,5). While de Silva and co-workers have reported spectrophotometric and EC/GC procedures which can sometimes measure the peak concentration of flurazepam following dosing (4,5), only the recently published RIA procedure of Glover et al (6) Cl H over a sufficient period of time to determine the drug's pharmacokinetic profile.…”

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confidence: 99%

Determination of flurazepam in human plasma by gas chromatography-electron capture negative chemical ionization mass spectrometry

Miwa¹,

Garland²,

Blumenthal³

1981

Anal. Chem.

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A specific and extraordinarily sensitive GC/MS assay has been developed to quantitate flurazepam, a commonly prescribed hypnotic, In human plasma. The assay requires 2 mL of plasma and features the use of electron capture negative chemical ionization. A decadeuterated analogue of flurazepam Is used as Internal standard. A 4 ft X 1 mm micropacked column containing OV-17 on juPartisorb Is used for the GC analysis. A quadruple mass spectrometer Is used to

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confidence: 99%

Determination of flurazepam in human plasma by gas chromatography-electron capture negative chemical ionization mass spectrometry

Miwa¹,

Garland²,

Blumenthal³

1981

Anal. Chem.

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“…Pharmacokinetic investigations using this assay method 10 yield somewhat different results than those in the tracer studies. 72 In a dog a 5 mg/kg intravenous dose was distributed within 10 minutes and biotransformed with a half-time of approximately 2.25 hours.…”

Section: Animal Pharmacologymentioning

confidence: 83%

Flurazepam hydrochloride

Greenblatt

Shader

Koch‐Weser

1975

Clin Pharma and Therapeutics

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“…The increased sensitivity limit for the 9-acridanone of flurazepam suggests that application of laser-induced fluorescence for its quantitation in biological fluids (21) could result in a fivefold improvement of sensitivity using the high-power mode, Table I.…”

Section: Resultsmentioning

confidence: 99%

“…In this study, laser-induced molecular fluorescence was investigated for the analysis of intrinsically fluorescent compounds such as quinine sulfate, salicylic acid, carprofen (a carbazole derivative under development as an antiinflammatory agent) (17,18), 2-methoxy-ll-oxo-llH-pyrido[2,lb]quinazoline-8-carboxylic acid, under development as an antiallergy agent (19), and fluorescent derivatives of nonfluorescent compounds such as the quinazolinone produced by the photolysis of demoxepam (a metabolite of the anxiolytic, chlordiazepoxide) (20), the 9-acridanone derivative of flurazepam (a hypnotic) (21), and the fluorescamine (Fluram) derivative of amphetamine (22). The sensitivity limit of laser-induced fluorescence was either equal to or up to tenfold greater than that obtained by conventional spectrofluorometry for the compounds studied.…”

mentioning

confidence: 99%