Determination of (fluoro)quinolone antibiotic residues in pig kidney using liquid chromatography-tandem mass spectrometry

Toussaint, B.; Chedin, Mostafa; Vincent, Ursula; Bordin, Guy; Rodríguez, A. R.

doi:10.1016/j.chroma.2005.02.016

Cited by 50 publications

(33 citation statements)

References 9 publications

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“…Various methods based on HPLC coupled with UV detection, fluorescence detection (FLD) or MS have been proposed for the analysis of quinolones in milk, eggs, pig and chicken [8][9][10][11][12][13][14][15][16][17][18][19]. Recently, Galarini et al [18] described a multi-residue procedure for assaying eleven quinolones in animal feed by FLD and UV.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of quinolones in fish by liquid chromatography coupled with fluorescence detection: Comparison of sub‐2 μm particles and conventional C₁₈ columns

Zou

Chen

et al. 2010

J of Separation Science

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A simple and effective multi-residue analysis method is presented for the extraction and determination of eleven quinolones (pipemidic acid, enoxacin, norfloxacin, ciprofloxacin, lomefloxacin, enrofloxacin, gatifloxacin, difloxacin, oxolinic acid, nalidixic acid and flumequine) in fish tissues. In this study, multi-residue separations on four columns packed with 5 microm or sub-2 microm particles were simultaneously developed for the purpose of comparison. Various gradients were optimized and best resolutions were achieved on each column. A short and sub-2 microm particle-sized HPLC column was chosen for its advantages in analysis time and column performance. Additionally, considering the matrix effect of the complex crude fish tissue, an effective extraction protocol was also established for sample pre-treatment procedure. Good recoveries (71-98%) were obtained from samples fortified with a mix of eleven quinolones at three levels, with satisfactory relative standard deviations and limits of detection. As a result, the sub-2 microm HPLC column and proposed analytical procedures have been evaluated and applied to the analysis of different fish tissues. Detectable residues were observed in 8 of 30 samples, at concentrations ranging from 4.74 to 23.27 microg/kg.

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Section: Introductionmentioning

confidence: 99%

Simultaneous determination of quinolones in fish by liquid chromatography coupled with fluorescence detection: Comparison of sub‐2 μm particles and conventional C₁₈ columns

Zou

Chen

et al. 2010

J of Separation Science

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“…However, these methods are complicated, timeconsuming, and specificity-lacking (Kurittu et al, 2000). Instrumental methods, including liquid chromatography-mass spectrometry (LC-MS) and highperformance liquid chromatography (HPLC) (Johnston et al, 2002;van Vyncht et al, 2002;EspinosaMansilla et al, 2005;Toussaint et al, 2005a;2005b;Zeng et al, 2005), are the most current widely used methods for CPFX residues detection. These methods are sensitive and highly specific, but they require expensive equipment, large volumes of solvents, and highly trained individuals for operating complicated instruments, with time-consuming sample cleanup processes.…”

Section: Introductionmentioning

confidence: 99%

Preparation of high-affinity rabbit monoclonal antibodies for ciprofloxacin and development of an indirect competitive ELISA for residues in milk

Huang

Yin

Lü

et al. 2010

J. Zhejiang Univ. Sci. B

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Abstract:A convenient competitive enzyme-linked immunosorbent assay (ELISA) for ciprofloxacin (CPFX) was developed by using rabbit monoclonal antibodies (RabMAbs) against a hapten-protein conjugate of CPFX-bovine serum albumin (BSA). The indirect competitive ELISA of CPFX had a concentration at 50% inhibition (IC 50 ) of 1.47 ng/ml and a limit of detection (LOD) of 0.095 ng/ml. The mAb exhibited some cross-reactivity, however, not so high with enrofloxacin (28.8%), ofloxacin (13.1%), norfloxacin (11.0%), fleroxacin (22.6%), and pefloxacin (20.4%). And it showed almost no cross-reactivity with other antibiotics or sulfonamides evaluated in this study. The competitive ELISA kit developed here could be used as a screening tool to detect and control illegal addition of CPFX in food products. This kit had been applied to milk detection and the recovery rates from samples spiked by CPFX were in a range of 63.02%-84.60%, with coefficients of variation of less than 12.2%.

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“…Most of them involve liquid chromatography with ultraviolet (LC-UV; Gigosos et al, 2000;Pecorelli et al, 2003;Bailac et al, 2004;Christodoulou and Samanidou, 2007), fluorescence (LC-FD;Eng et al, 1998;Hernández-Arteseros et al, 2000;Chu et al, 2002;EspinosaMansilla et al, 2005;Hassouan et al, 2007) or mass spectrometric detection (Van Vyncht et al, 2002;Toussaint et al, 2002Toussaint et al, , 2005aSchneider et al, 2005;Van Hoof et al, 2005;Hermo et al, 2006;Bailac et al, 2006;Rubies et al, 2007).…”

Section: Introductionmentioning

confidence: 98%

Simultaneous determination of quinolone antibacterials in bovine milk by liquid chromatography–mass spectrometry

Zafra-Gómez

Garballo

Ballesteros

et al. 2008

Biomedical Chromatography

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A new liquid chromatography-mass spectrometry (LC-MS) method has been developed and validated for the simultaneous determination of eight quinolone antibacterials for veterinary use in processed bovine milk samples. The quinolones studied included marbofloxacin, ciprofloxacin, danofloxacin, enrofloxacin, sarafloxacin, difloxacin, oxolinic acid and flumequine. Also, a new sample-treatment procedure was used for extraction and preconcentration of these compounds. It involved defatting by centrifugation, protein precipitation by adding a mixture of glacial acetic acid-acetonitrile and removing acetonitrile with dichloromethane; finally, the acidified aqueous layer was evaporated to dryness in a speed vac system, resuspended in the mobile phase and filtered prior to LC injection. The mobile phase was composed of a formic acid aqueous solution 0.1% (v/v) and acetonitrile, with an initial composition of water-acetonitrile 95: 5 (v/v) and using linear gradient elution. Norfloxacin was used as internal standard. The limits of quantification found (2-7 ng g(-1)) were in all cases lower than the maximum residue limits tolerated by the European Union for these compounds in milk.

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Determination of (fluoro)quinolone antibiotic residues in pig kidney using liquid chromatography-tandem mass spectrometry

Cited by 50 publications

References 9 publications

Simultaneous determination of quinolones in fish by liquid chromatography coupled with fluorescence detection: Comparison of sub‐2 μm particles and conventional C₁₈ columns

Simultaneous determination of quinolones in fish by liquid chromatography coupled with fluorescence detection: Comparison of sub‐2 μm particles and conventional C₁₈ columns

Preparation of high-affinity rabbit monoclonal antibodies for ciprofloxacin and development of an indirect competitive ELISA for residues in milk

Simultaneous determination of quinolone antibacterials in bovine milk by liquid chromatography–mass spectrometry

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Determination of (fluoro)quinolone antibiotic residues in pig kidney using liquid chromatography-tandem mass spectrometry

Cited by 50 publications

References 9 publications

Simultaneous determination of quinolones in fish by liquid chromatography coupled with fluorescence detection: Comparison of sub‐2 μm particles and conventional C18 columns

Simultaneous determination of quinolones in fish by liquid chromatography coupled with fluorescence detection: Comparison of sub‐2 μm particles and conventional C18 columns

Preparation of high-affinity rabbit monoclonal antibodies for ciprofloxacin and development of an indirect competitive ELISA for residues in milk

Simultaneous determination of quinolone antibacterials in bovine milk by liquid chromatography–mass spectrometry

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Simultaneous determination of quinolones in fish by liquid chromatography coupled with fluorescence detection: Comparison of sub‐2 μm particles and conventional C₁₈ columns

Simultaneous determination of quinolones in fish by liquid chromatography coupled with fluorescence detection: Comparison of sub‐2 μm particles and conventional C₁₈ columns