Determination of flavonoid level variation in onion (Allium cepa L.) infected by Fusarium oxysporum using liquid chromatography–tandem mass spectrometry

Lee, Jung Han; Lee, Soo Jung; Park, Semin; Jeong, Sung Woo; Kim, Chi Yeon; Jin, Jong Sung; Jeong, Euh‐Duck; Kwak, Youn‐Sig; Kim, Soo Taek; Bae, Dong Won; Kim, Gon‐Sup; Shin, Sung Chul

doi:10.1016/j.foodchem.2012.02.063

Cited by 19 publications

(13 citation statements)

References 20 publications

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“…Peak 4 exhibited typical flavonol UV-vis spectral characteristics and a pseudo-molecular ion at m/z = 627, a diagnostic fragment at m/z = 465 (loss of a glucosyl moiety) and a fragment at m/z = 303 corresponding to the aglycone. This compound was identified as quercetin 7,4 -O,O-diglucoside (Lee et al, 2012). Likewise, peak 6 gave an identical fragmentation pattern and it was assigned to quercetin 3,4 -O,O-diglucoside (Kiassos et al, 2009;Lee et al, 2012).…”

Section: Polyphenolic Profile and Reducing Power (P R )mentioning

confidence: 93%

“…This compound was identified as quercetin 7,4 -O,O-diglucoside (Lee et al, 2012). Likewise, peak 6 gave an identical fragmentation pattern and it was assigned to quercetin 3,4 -O,O-diglucoside (Kiassos et al, 2009;Lee et al, 2012). Peak 8 with pseudo-molecular ion at m/z = 465 and a fragment at m/z = 303, as well as peak 10 with pseudo-molecular ion at m/z = 303 were identified as quercetin 4 -O-glucoside and quercetin, respectively.…”

Section: Polyphenolic Profile and Reducing Power (P R )mentioning

confidence: 99%

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A green ultrasound-assisted extraction process for the recovery of antioxidant polyphenols and pigments from onion solid wastes using Box–Behnken experimental design and kinetics

Katsampa

Valsamedou

Grigorakis

et al. 2015

Industrial Crops and Products

137

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Section: Polyphenolic Profile and Reducing Power (P R )mentioning

confidence: 93%

Section: Polyphenolic Profile and Reducing Power (P R )mentioning

confidence: 99%

A green ultrasound-assisted extraction process for the recovery of antioxidant polyphenols and pigments from onion solid wastes using Box–Behnken experimental design and kinetics

Katsampa

Valsamedou

Grigorakis

et al. 2015

Industrial Crops and Products

137

View full text Add to dashboard Cite

“…Metabolomics analyses with different plant resources showed that flavonoids form a key stone in defending plants against adverse conditions by reducing the accumulation of ROS triggered by biotic or abiotic stress [7,[51][52][53]. A recent report by Lee et al, [55] has shown an increase in the flavonoid level after Fusarium pathogen inoculation of onion bulbs, providing an important highlight towards the functional role of the flavonoids in the onion defense against FOC. In the present study, T1-and T1+FOC-treated onion plants stimulated the accumulation of quercetin 3-O glucoside, quercetin 4´-O-glucoside, quercetin and kaempferol ( Fig.…”

Section: Thus Our Data Have Made a Clear Discrimination Between The mentioning

confidence: 96%

Dissection of Trichoderma longibrachiatum-induced defense in onion (Allium cepa L.) against Fusarium oxysporum f. sp. cepa by target metabolite profiling

et al. 2016

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Trichoderma spp. are versatile opportunistic plant symbionts that can cause substantial changes in the metabolism of host plants, thereby increasing plant growth and activating plant defense to various diseases. Target metabolite profiling approach was selected to demonstrate that Trichoderma longibrachiatum isolated from desert soil can confer beneficial agronomic traits to onion and induce defense mechanism against Fusarium oxysporum f. sp. cepa (FOC), through triggering a number of primary and secondary metabolite pathways. Onion seeds primed with Trichoderma T1 strain displayed early seedling emergence and enhanced growth compared with Trichoderma T2-treatment and untreated control. Therefore, T1 was selected for further investigations under greenhouse conditions, which revealed remarkable improvement in the onion bulb growth parameters and resistance against FOC. The metabolite platform of T1-primed onion (T1) and T1-primed onion challenged with FOC (T1+FOC) displayed significant accumulation of 25 abiotic and biotic stress-responsive metabolites, representing carbohydrate, phenylpropanoid and sulfur assimilation metabolic pathways. In addition, T1- and T1+FOC-treated onion plants showed discrete antioxidant capacity against 1,1-diphenyl-2-picrylhydrazyl (DPPH) compared with control. Our findings demonstrated the contribution of T. longibrachiatum to the accumulation of key metabolites, which subsequently leads to the improvement of onion growth, as well as its resistance to oxidative stress and FOC.

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“…Compound (3) exhibited typical fl avonol UV-Vis spectral characteristics and a pseudomolecular ion at m/z=627, a diagnostic fragment at m/z=465 (loss of a glucosyl moiety), and a fragment at m/z=303 corresponding to the aglycone. This compound was identifi ed as quercetin 7,4´-O,O-diglucoside (LEE et al, 2012). Likewise, compound (4) gave an identical fragmentation pattern and it was assigned to quercetin 3, 4´-O,O-diglucoside (KIASSOS et al, 2009;LEE et al, 2012).…”

Section: Polyphenolic Compositionmentioning

confidence: 99%

“…This compound was identifi ed as quercetin 7,4´-O,O-diglucoside (LEE et al, 2012). Likewise, compound (4) gave an identical fragmentation pattern and it was assigned to quercetin 3, 4´-O,O-diglucoside (KIASSOS et al, 2009;LEE et al, 2012). Compound (5) with pseudo-molecular ion at m/z=465 and a fragment at m/z=303, as well as compound (6) with pseudo-molecular ion at m/z=303 were identifi ed as quercetin 4´-O-glucoside and quercetin, respectively.…”

Section: Polyphenolic Compositionmentioning

confidence: 99%

Kinetic modelling for polyphenol extraction from onion (Allium cepa) solid wastes using acidified water/ethanol mixture

Makris¹,

Kefalas²

2015

Acta Alimentaria

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Previous reports highlighted the onion solid wastes as abundant, residual material that might contain a signifi cant load of antioxidant polyphenols. Although there have been studies pertaining to polyphenol recovery from onion wastes, the effect of temperature has not been adequately addressed. In this line, this study was undertaken with the aim of establishing a correlation between the extraction yield in total polyphenols and the extraction temperature, using acidifi ed aqueous ethanol as the solvent system. Extraction of polyphenols from onion solid wastes was found to obey 2 nd -order kinetics. On such a basis, the yield in total polyphenols at saturation could be very effectively determined and correlated with temperature using non-linear regression. The results indicated that the extraction yield at saturation is highly correlated with temperature, following a quadratic function. The extract obtained at optimal temperature (40 °C) had a total polyphenol yield of 21.10 mg gallic acid equivalents per gram of dry weight, and it was further analysed by liquid chromatography-mass spectroscopy to characterise its major constituents. The polyphenols detected were quercetin glucosides, as well as quercetin oxidation derivatives, including certain degradation products and dimers. The outcome of this study outlined that temperatures above 40 °C are rather not favourable for polyphenol extraction from onion solid wastes, as suggested by the model established through kinetics. The extract obtained under optimal conditions contained peculiar polyphenolic composition, not encountered in any other food processing residue.

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Determination of flavonoid level variation in onion (Allium cepa L.) infected by Fusarium oxysporum using liquid chromatography–tandem mass spectrometry

Cited by 19 publications

References 20 publications

A green ultrasound-assisted extraction process for the recovery of antioxidant polyphenols and pigments from onion solid wastes using Box–Behnken experimental design and kinetics

A green ultrasound-assisted extraction process for the recovery of antioxidant polyphenols and pigments from onion solid wastes using Box–Behnken experimental design and kinetics

Dissection of Trichoderma longibrachiatum-induced defense in onion (Allium cepa L.) against Fusarium oxysporum f. sp. cepa by target metabolite profiling

Kinetic modelling for polyphenol extraction from onion (Allium cepa) solid wastes using acidified water/ethanol mixture

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