Determination of Flavonoid Content in Tapak Dara Root Decoction (Catharanthus Roseus)

Abstract

Catharanthus roseus is a plant that grows wild and is widely used by people as an ornamental plant. This plant is known to have antibacterial, antioxidant, antifungal, anthelmintic, antineoplastic, antihyperglycemic, anticancer, antidiarrheal and antiviral activities so it is used to treat various diseases such as malaria, constipation, cancer, diuretics, diabetes mellitus, cholesterol, and hypotension. This research aims to determine the levels of flavonoids in the roots of the Tapak Dara plant (Catharanthus roseus) with varying concentrations of 5%, 10% and 15%. The method used in this research is the dekokta method. Determination of decok flavonoid levels in the roots of the Tapak Dara plant (Catharanthus roseus) done by adding AlCl reagent3 then analyzed using a UV-vis spectrophotometer with a wavelength of 431 nm. The average yield of Tapak Dara plant root flavonoids obtained with a concentration of 5% was 0.18±0.0015 mg/L, 10% was 0.76±0.0020 mg/L and 15% was 2.31±0.0015 mg/L. The conclusion of this study shows that the greater the sample concentration, the greater the flavonoid content.