Determination of Flavonoid and Polyphenol Compounds in Viscum Album and Allium Sativum Extracts

Abstract

Ethnopharmacology is a new interdisciplinary science that appeared in Europe of the ‘90, in France, as a necessity claimed by the return to the traditional remedies of each nation. The aim of this study is to identify and quantify the active ingredients of the species Viscum album and Allium sativum, in order to provide a complex chemical characterisation, which is necessary for the use of these plants’ extracts as natural ingredients in the pharmaceutical industry. The following methods were used: (1) the plant material was harvested from the west-side of Romania (Europe) in July 2014; (2) it was dried quickly and the main active principles were extracted using ethylic alcohol solution (50%); (3) the quantitative analyses of the flavonoids and polyphenols were performed according to a procedure described in the Romanian Pharmacopoeia. FT-IR results showed that the Viscum album extract had the highest content of polyphenolic compounds, for both flavonoids and polyphenols. This is the reason why it can be concluded that alcoholic extracts of mistletoe must be used as supplements for diabetics who require diets with flavonoids or for patients with cancers, degenerative diseases, and particularly cardiovascular diseases, who need an increased amount of polyphenols.DOI: http://dx.doi.org/10.3329/icpj.v4i5.22861 International Current Pharmaceutical Journal, April 2015, 4(5): 382-385

Evaluation and comparison of the content of total polyphenols and antioxidant activity of selected species of the genus Allium

“…Its best known chemical compound is allicin, which has extensive pharmacological effects (Trifunschi et al, 2015). Today, in many parts of the world garlic is being used as prophylaxis and for the cure of numbers of diseases including acute and chronic infections (Srivastava and Pathak, 2012).…”

Optimising chitosan–pectin hydrogel beads containing combined garlic and holy basil essential oils and their application as antimicrobial inhibitor

“…In the FTIR spectra, the peaks at 3265-3339 and 3462 cm À1 (strong for GEO) signifies the O-H stretching vibrations of hydroxyl groups, which is attributed to polyhydroxy compounds, such as flavonoids, non-flavonoids and saponins (Katata-Seru et al, 2017). Asymmetric stretching of C-H groups of aromatic compounds (2926-2979 cm À1 ) and the O-H bending of carboxylic acids (1384-1395 cm À1 ) (Trifunschi et al, 2015;Divya et al, 2017) was seen, as well as the S=O group (1036 cm À1 ) corresponding to organosulphur compounds, including alliin, allicin and DADS (Divya et al, 2017). Characteristic vibration frequencies in the FTIR of O. sanctum are attributed to O-H groups (3369-3428 cm À1 ), C=C stretching vibrations in the aromatic rings (1509-1612 cm À1 ) (Balamurughan et al, 2014;Sutaphanit & Chitprasert, 2014), C-H bending vibrations (1450 cm À1 ), and O-H bending vibrations in phenols (1268 cm À1 ).…”

Uji Fitokimia Senyawa Metabolit Sekunder Dalam Ekstrak Metanol Bunga Turi Merah (Sesbania grandiflora L. Pers)

“…Puncak pada bilangan gelombang 1406,265 cm -1 juga merupakan representasi regangan C=C cincin aromatis yang mengalami resonansi sehingga menghasilkan serapan dengan bilangan gelombang yang lebih rendah dari rentang 1650-1430 cm −1 (Stuart, 2007dan Klein, 2012. Karakteristik khas keberadaan ikatan C-O senyawa fenolik ditunjukkan oleh puncak dengan intensitas yang kuat pada bilangan gelombang 1046,955 cm -1 yang menandakan regangan ikatan antara C-O (Trifunschi et al, 2015dan Stuart, 2007. Tampilan hasil uji dengan FTIR ini disajikan pada Gambar 3.…”

“…Hal ini mengakibatkan absorbsi beberapa gugus atom terhadap inframerah sangat rendah sehingga puncaknya tidak muncul dan terjadi peaks overlapping dalam spektra tersebut dari senyawasenyawa yang ada dalam sampel (Stuart, 2007). Kecenderungan ini juga terlihat dari beberapa hasil penelitian spektroskopi IR tentang senyawa fenolik menggunakan crude extract (CruzEspinoza et al, Deepa et al, 2014, dan Trifunschi et al, 2015. Pola lokasi bilangan gelombang, bentuk, dan intensitas spektra di penelitian ini juga mirip dengan hasil beberapa penelitian tentang elusidasi senyawa fenolik dari crude extract tumbuhan yang berbeda.…”

“…Keberadaan cincin aromatis senyawa tersebut ditegaskan melalui uji spektrofotometri UV-Vis. Trifunschi et al (2015) Berdasarkan analisis menggunakan spektroskopi IR pada ekstrak metanol bunga turi merah ini, gugus fungsi yang terbaca adalah hidroksida, olefin, dan aromatis. Fakta ini yang didukung oleh beberapa referensi analisis senyawa fenolik menegaskan bahwa ekstrak metanol bunga turi merah mengandung senyawa fenolik.…”