Determination of Five Coumarins in Cnidii Fructus by Micellar Electrokinetic Capillary Chromatography

Abstract

A micellar electrokinetic capillary chromatography (MEKC) method for the separation and quantification of the coumarins in Cnidii Fructus was developed for the first time. Within 25 minutes, 5 major coumarins were separated using 18 mM borate, 12 mM phosphate and 50 mM SDS (pH 9.2) containing 20 % methanol. The relative standard deviations of migration times and peak areas were less than 5 %. The total contents of the five coumarins in plants from different habitats ranged from 13.6 to 44.6 mg/g, and recoveries ranged from 96.0 to 102.8 %. The effects of buffer concentration, SDS, pH value and organic modifier on the separation were investigated.

Fingerprint analysis of the fruits of Cnidium monnieri extract by high-performance liquid chromatography–diode array detection–electrospray ionization tandem mass spectrometry

“…Based on the m/z value, UV spectra and the comparison with standard compounds, five peaks were unambiguously identified as xanthotoxin (8), isopimpinelline (11), bergapten (12), imperatorin (17), and osthole (18). Other 15 peaks were tentatively identified as diosmetin (1), cnidimol B (2), dl-umtatin (3), cnidimol A (4), cnidimol E (5), cnidimol D (6), cnidimol C (7), demethyl-auraptenol (9), auraptenol (10), 5-formylxanthotoxol (13), 2 -hydrate-deoxymeranin (14), cnidiadin (15), oroselone (16), cniforin A (19), and edultin (20) by comparing their m/z value and UV spectra with the literature data [26][27][28][29][30][31][32][33][34]. The results are listed in Table 1 and the structures of these compounds are shown in Fig.…”

“…A variety of methods including high-performance liquid chromatography (HPLC) [12], gas chromatography (GC) [13], and micellar electrokinetic capillary chromatography (MEKC) [14] have been used for characterization of the coumarins in the fruits of C. monnieri. However, these separations only dealt with the five main coumarin components in the crude drug.…”

Fast separation and determination of coumarins in Fructus cnidii extracts by CEC using poly(butyl methacrylate‐co‐ethylene dimethacrylate‐co‐[2‐(methacryloyloxy)ethyl] trimethylammonium chloride) monolithic columns

“…However, long analysis time (at least 25 min) and gradient elution were needed, and high amount of organic modifiers was used during the separation [25][26][27]. Some articles have also reported that other analytical methods had been established successfully based on GC [28,29] and MEKC [23]. Even using the widely acceptable fast separation method as MEKC, the analysis time was still as long as 20 min.…”

“…It also possesses anti-tumor activity [22]. These constituents are regarded as the pharmacological and biochemical active constituents and often used as standards in the quantitative control of F. cnidii [23,24]. Their chemical structures are shown in Fig.…”

Identification and quantification of the volatile constituents in Cnidium monnieri using supercritical fluid extraction followed by GC‐MS

“…Methods developed for the analysis of the coumarins in the fruits of C. monnieri include HPLC [6], GC [7], MEKC [8], and HPLC-diode array detection (DAD)-ESI-MS/MS [9]. Owing to high sensitivity combined with the possibility of achieving efficient separations of complex mixtures, GC-FID and GC-MS have gained general acceptance for the analysis of coumarins.…”