Determination of fentanyl in biological and water samples using single-drop liquid–liquid–liquid microextraction coupled with high-performance liquid chromatography

Ebrahimzadeh, Homeira; Yamini, Yadollah; Gholizade, Amir; Sedighi, Abootaleb; Kasraee, S.

doi:10.1016/j.aca.2008.07.047

Cited by 56 publications

(26 citation statements)

References 31 publications

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“…Methods using HPLC-UV, [12][13][14][15] GC-MS, 16) and LC-MS, [17][18][19][20][21][22] have been used to determine the fentanyl concentration in plasma [18][19][20]22) and breast milk. 21) Liquid chromatographytandem mass spectrometry (LC-MS/MS) provides a rapid, sensitive, and specific analysis.…”

mentioning

confidence: 99%

A Simple Liquid Chromatography–Tandem Mass Spectrometry Method for Determination of Plasma Fentanyl Concentration in Rats and Patients with Cancer Pain

Hisada

Katoh

Hitoshi

et al. 2013

Biological & Pharmaceutical Bulletin

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A fentanyl patch is widely used for the treatment of cancer pain. Its few adverse effects include constipation and drowsiness. The absorption volume of transdermally applied fentanyl may differ according to its site of application and variability in patch adhesion. Since fentanyl is predominantly metabolized by the drug-metabolizing enzyme cytochrome P450 (CYP) 3A4 in the liver, its concentration may vary in cases of physiologically reduced CYP3A4 activity in the liver (liver disease and aging) or on co-administration of drugs. The clinical significance of measuring plasma concentration of fentanyl is high, but conventional methods require complicated processes such as solid-phase extraction and liquid-liquid extraction before the sample is injected into an HPLC system. In this study, a simple liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for determining plasma fentanyl concentrations by deproteinization with acetonitrile. A recovery test was conducted using an absolute calibration curve to confirm the method's linearity and inter-and intra-day reproducibility. The required plasma volume for detection was reduced from 1 mL in the conventional method to 20 µL in the present study, and a good calibration curve was obtained in the concentration range from 0.05 to 5 ng/mL. These findings suggest that the method for sample preparation and quantification developed in this study are appropriate for measuring fentanyl concentration in human plasma in clinical settings.

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mentioning

confidence: 99%

A Simple Liquid Chromatography–Tandem Mass Spectrometry Method for Determination of Plasma Fentanyl Concentration in Rats and Patients with Cancer Pain

Hisada

Katoh

Hitoshi

et al. 2013

Biological & Pharmaceutical Bulletin

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“…LLME that was reported previously is requiring pre-extraction and back-extraction method combined to the tedious micro-droplet acceptor phase, and takes 50 min for extraction procedure only [10], whereas this method using simple liquid extraction makes it the handiest method to perform, that takes 35 min to obtain the final peak on the chromatogram. With these qualities, this method can be used for FEN pharmacokinetic assessment in rats, FEN drug interactions studies and will give possible use for forensic purposes after slight modifications.…”

Section: Resultsmentioning

confidence: 99%

“…These methods included different types of extraction procedures such as liquid-liquid extraction (LLE) utilizing hexane to extract target analytes [6,7], solid phase extraction (SPE) [9] and single-drop liquid-liquid-liquid micro-extraction (LLME) [10].…”

Section: Introductionmentioning

confidence: 99%

HPLC-UV method development for fentanyl determination in rat plasma and its application to elucidate pharmacokinetic behavior after i.p. administration to rats

Almousa

Ikeda

Wada

et al. 2011

Journal of Chromatography B

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A simple, rapid and validated high performance liquid chromatography method with UV detection for the quantification of an opioid agonist, fentanyl (FEN), in rat plasma was developed. The assay procedure involved chromatographic separation using a ZIC-HILIC SeQUANT column (250 × 4.6 mm, i.d., 5 µm) and a mobile phase of acetonitrile and acetate buffer (pH 3.4, 20 mM) of ratio (= 65:35, v/v) at a flow rate of 1.2 mL/min and detection wavelength of 201 nm. Plasma sample (100 μL) pretreatment was based on simple deprotienization by acetonitrile spiked with clonidine as an internal standard (I.S.) of 20 ng/mL followed by extraction with tert-butyl methyl ether and centrifugation. The organic layer was evaporated under N 2 gas and reconstituted with 100 μL of acetate buffer (pH 3.4, 20 mM), and 50-μL portions of reconstituted sample were injected onto the column. Sample analysis including sample pretreatment was achieved within 35 min. Calibration curve was linear (r ≥ 0.998) from 5 to 100 ng/mL. Both intra-and inter-day assay precisions that are presented through R.S.D were lower than 12.6% for intra-day and lower than 12.0% for inter-day assessment. Limit of detection was 0.8 ng/mL at S/N of 3. This method was omitting the use of expensive solid phase extraction and time consuming liquid extraction procedures. Moreover, the present method was successfully applied to study pharmacokinetic parameters of FEN after intraperitoneal administration to male Wistar rat. Pharmacokinetic parameters estimated by using moment analysis were; T 1/2 198.3 ± 44.7 min, T max 28.3 ± 2.9 min and AUC 0-180 15.6 ± 2.9 (×10 2 ) ng·min/mL.

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“…Due to the interferences of co-extractives from urine sample, the acceptor phase drop was found unstable and easily lost during LLLME procedure. In order to diminish the matrix effect and consequently to improve the stability of droplet, the urine samples were diluted at 2:3 ratios with sodium hydroxide 0.5 mol/L [10,19]. The percent recovery was found in the range of 94.6 -101.0%.…”

Section: Methods Validationmentioning

confidence: 99%

Determination of anti‐malaria agent chloroquine using single drop liquid‐liquid‐liquid microextraction

Daneshfar

Khezeli

Manafi

2009

J of Separation Science

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A simple, sensitive, and inexpensive single drop liquid-liquid-liquid microextraction combined with isocratic RP-HPLC and UV detection was developed for the determination of anti-malaria drug, chloroquine. The target compound was extracted from alkaline aqueous sample solution (adjusted to 0.5 mol/L sodium hydroxide) through a thin layer of organic solvent membrane and back-extracted to an acidic acceptor drop (adjusted to 0.02 mol/L phosphoric acid) suspended on the tip of a 25 microL HPLC syringe in the organic layer. This syringe was also used for direct injection after extraction. The linear range was 1-200 microg/L. The LOD and LOQ were 0.3 and 1.0 microg/L, respectively. Intra-and inter-day precisions were less than 2.0 and 2.3%, respectively. The real samples were successfully analyzed using the proposed method. The recoveries of spiked samples were more than 94.6%.

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Determination of fentanyl in biological and water samples using single-drop liquid–liquid–liquid microextraction coupled with high-performance liquid chromatography

Cited by 56 publications

References 31 publications

A Simple Liquid Chromatography–Tandem Mass Spectrometry Method for Determination of Plasma Fentanyl Concentration in Rats and Patients with Cancer Pain

A Simple Liquid Chromatography–Tandem Mass Spectrometry Method for Determination of Plasma Fentanyl Concentration in Rats and Patients with Cancer Pain

HPLC-UV method development for fentanyl determination in rat plasma and its application to elucidate pharmacokinetic behavior after i.p. administration to rats

Determination of anti‐malaria agent chloroquine using single drop liquid‐liquid‐liquid microextraction

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