Determination of fenbendazole and its metabolites in milk by the method of liquid chromatography coupled with tandem mass-spectrometry

Abstract

<jats:p>Objective of research: Development of methods for the determination of fenbendazole and its metabolites in milk by liquid chromatography coupled with tandem mass spectrometry. &#x0D; Materials and methods: Fenbendazole was administered orally to five cows. Samples of milk were taken on 1, 3, 5 and 10 days of drug application. The research method includes a description of reagents, plates and equipment; mass-spectrometric conditions for analysis of fenbendazole and its metabolites; preparation of the equipment to operation; preparation of eluent solution; preparation of the chromatograph to analysis; determination of chromatographic parameters of standard drug samples; preparation of milk samples to analysis; establishment of parameters of extracts’ chromatography; procedure of calibration of fenbendazole and its metabolites in eluent.&#x0D; Results and discussion: When studying the pharmacokinetics of fenbendazole and its metabolites (sulfone and sulfoxide) in milk, it was found that the maximal concentrations were determined 24 h after drug administration and were 22,6 ng/ml for fenbendazole sulfone, 34,0 ng/ml for fenbendazole sulfoxide and 19,7 ng/ml for fenbendazole. 10 days after treatment, the concentrations of fenbendazole and its metabolites in milk did not exceed permitted values.</jats:p>

Biological Activity of Fenbendazole Based on Supramolecular Delivery System With Disodium Salt of Glycyrrhizic Acid

“…Параметры фармакокинетики фенбендазола и его метаболитов в организме овец породы ставропольский меринос изучали методом высокоэффективной жидкостной хроматографии с тандемным масс-спектро-метрическим детектированием (24). Для опыта сформировали две группы клинически здоровых овец (по 5 гол.…”

“…SDF with Na2GA and the substance of fenbendazole (FBZ) was administered to two groups of clinically healthy sheep (5 animals each) once per or at the dose of 2 mg/kg of active substance. Blood samples were taken from the jugular vein 0, 1,2,4,6,8,12,24,33,48,72, and 144 hours after administration of SDF with Na2GA and the basic drug. The absorption rate constant, absorption half-life, clearance of the drug from the blood plasma, maximum drug concentration, time to reach maximum plasma drug concentration following drug administration, elimination half-life, area under the concentrationtime curve and mean residence time were calculated.…”