Determination of Faecal Fat by Near-Infrared Spectroscopy

Bekers, Otto; Postma, C.J.; Lombarts, A.J.P.F.

doi:10.1515/cclm.1995.33.2.83

Cited by 6 publications

(3 citation statements)

References 10 publications

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“…Therefore, the applicability of this equipment is hampered by the necessity of substantial numbers of samples to be analysed in order to justify the purchase of the apparatus. 5 Franck et al 8 described the use of a FT-IR method, employing an ATR sampling device, for the prediction of faecal fat. They made preliminary sample selections, based on the faecal water content and the sample homogeneity.…”

Section: Discussionmentioning

confidence: 99%

“…These are biological samples from which the sample compositions have been determined by means of a generally accepted method, such as that of Van de Kamer. However, Bekers et al 5 highlighted the need to create a new calibration curve in every clinical chemical laboratory when using secondary reference samples. A paper describing a multicentre trial showed that the results of the Van de Kamer method were poorly exchangeable.…”

Section: Introductionmentioning

confidence: 99%

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Investigation of applicability of a mid-infrared spectroscopic method using an attenuated total reflection accessory and a new near-infrared transmission method for determination of faecal fat

Volmer

Kingma²,

Borsboom³

et al. 2001

Ann Clin Biochem

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SUMMARY. In many laboratories, the titrimetric method of Van de Kamer is used for the analysis of faecal fat content of patients suspected of steatorrhoea. We investigated the applicability of a mid-infrared (MIR) spectroscopic method, using an attenuated total re¯ection (ATR) accessory, and a new near-infrared (NIR) spectroscopic method. For the NIR method, sealed plastic bags containing the stool samples were used as transmission cells. Standardization was obtained using a previously described MIR method, with a NaCl¯ow-cell, as reference method. Partial least-squares regression was used for the calibration of each method. Full cross-validation of the calibration set was used for the internal validation of each method. Fifteen per cent of the stool samples could not be estimated with the ATR method within reasonable accuracy limits compared with the reference. The standard error of prediction of the NIR method was 1´1 g/dL. We conclude that the new NIR method is a promising technique for routine use. However, further experiments need to be done with triplicate measurements of each sample and the use of an external validation set.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Investigation of applicability of a mid-infrared spectroscopic method using an attenuated total reflection accessory and a new near-infrared transmission method for determination of faecal fat

Volmer

Kingma²,

Borsboom³

et al. 2001

Ann Clin Biochem

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show abstract

“…They were reported to have 100% sensitivity and 96% specificity, but radioisotopes or nonradioactive isotopes and facilities for their determination are not available in most laboratories and the results are very expensive. Recently, near infrared spectrometry and NRM spectrometry were shown to be very sensitive and specific methods to determine the amount of fat in feces [111]. The steatocrit is a very interesting, simple, inexpensive, rapid, semiquantitative micromethod that uses an aliquot of homogeinized stool and centrifugation of the sample to determine the percentage of fat in the specimen [109].…”

Section: Tests For Detecting Functional Abnormalities Fat Malabsorptimentioning

confidence: 99%

Malabsorption Syndromes

Bai¹

1998

Digestion

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Background/Aims: Malabsorption syndromes commonly result from a pathological interference of the normal digestive process. There have been major advances in the last 4 years. The purpose of this review is to highlight in the form of a brief summary the most outstanding information available. Methods: The review was performed based on a medical literature search using MEDLINE (1993–1997), bibliographic reviews of book chapters and review articles. As a consequence of the extensive information incorporated in the period and the limited scope of this review, the review will focus in three aspects: (1) an overview on some clinical aspects of malabsorption; (2) diseases in which predominates the disturbed mucosal phase of the digestive process, and (3) providing information on diagnostic testing regarding malabsorption. Results: Major advances on celiac disease, Whipple’s disease, giardiasis, tropical sprue, malabsorption of oligo- and disaccharides, vitamin B₁₂ and bile salts are discussed. New aspects on diagnostic procedures for malabsorption are also presented. Conclusion: Although major advances have given a great support to the investigation of malabsorption, yet the syndrome remains a major diagnostic dilemma. Based on the limited availability of most diagnostic tests, a simple and practical diagnostic algorithm is presented.

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Determination of fecal fat concentration by near infrared spectrometry for the screening of pancreatic steatorrhea

Ventrucci

Cipolla

et al. 1998

International Journal of Pancreatology

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When mean 3-d or daily fat fecal output were considered, a strict linear relationship was found between NIRA and the colorimetric method (r = 0.97 and 0.94, respectively). Using fat concentration, the two tests correlated less well (r= 0.74). Fat concentration was significantly higher in pancreatic than in nonpancreatic steatorrhea, even though values overlapped widely, and thus discrimination was not possible. The diagnostic efficiency of fat concentration for pancreatic and nonpancreatic steatorrhea was 72 and 61%, respectively.

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Determination of Faecal Fat by Near-Infrared Spectroscopy

Cited by 6 publications

References 10 publications

Investigation of applicability of a mid-infrared spectroscopic method using an attenuated total reflection accessory and a new near-infrared transmission method for determination of faecal fat

Investigation of applicability of a mid-infrared spectroscopic method using an attenuated total reflection accessory and a new near-infrared transmission method for determination of faecal fat

Malabsorption Syndromes

Determination of fecal fat concentration by near infrared spectrometry for the screening of pancreatic steatorrhea

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