Determination of Extraction Buffer and Ammonium Sulfate Percentage for Pollen Crude Protein Extracts of Mangifera, Durio and Syzygium Fruit Flowers
Abstract:Flowering trees are considered the producers of allergenic agent carried by pollen protein. The isolation of protein of interest from intracellular compartment into a solution of well-defined composition especially from never isolated species involves the manipulation of several parameters. The aim of this work is to find the best isolation method for pollen protein of the widely distributed flowering fruit species in Malaysia includes Mangifera indica, Mangifera odorata, Durio graviolens, Durio zibethinus, Sy… Show more
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