Determination of Extended-Spectrum β-Lactamases and AmpC Production in Uropathogenic Isolates of Escherichia coli and Susceptibility to Fosfomycin

Gupta, Varsha; Rani, Hena; Singla, Nidhi; Kaistha, Neelam; Chander, Jagdish

doi:10.4103/0974-2727.119849

Cited by 40 publications

(45 citation statements)

References 13 publications

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“…This result was in agreement with another study conducted by Gupta et al, (2013) who showed that of 52.6% of the isolates were ESBLs producer strains and 10% of strains were AmpC producer. Another investigation from Egypt revealed that 57.7% of isolates were Amp C enzyme producer (Fam et al, 2013 Singhal et al, (2005), who reported 5.7%, 5.9%, and 7% of the isolate were AmpC producer respectively.…”

Section: Resultssupporting

confidence: 83%

“…Resistance to quinolones was recorded to be 91.1% to norfloxacin and 75.6% to ciprofloxacin. This was in consistent with Farzana et al, (2013), Gupta et al, (2013) and Chander and Shrestha (2013), who reported high level of resistance to quinolones more than 90%. Elsayed et al, (2017) reported similar resistance to ciprofloxacin (72.2%) but less resistance to norfloxacin (59%).…”

Section: Resultssupporting

confidence: 81%

“…Another investigation from Egypt revealed that 57.7% of isolates were Amp C enzyme producer (Fam et al, 2013 Singhal et al, (2005), who reported 5.7%, 5.9%, and 7% of the isolate were AmpC producer respectively. Gupta et al, (2013) found that not all AmpC positive strains were co-producer of ESBL and AmpC, but only 8% of the strains were co-producers of ESBL and AmpC, but in our study all AmpC positive isolates werecoproducers of ESBL and AmpC.…”

Section: Resultsmentioning

confidence: 54%

“…In ESBL producing strains, resistance was 72.5%. This relatively high rate of resistance was reported in another studies carried by Elsayed et al, (2017) and Gupta et al, (2013), however Chander and Shrestha (2013) stated a lower resistance rate (11.7%).…”

Section: Resultsmentioning

confidence: 70%

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Prevalence of Multidrug Resistant Uropathogenic E. coli: Role of β-lactamase

Gouda¹,

Zeid²

2017

Int.J.Curr.Microbiol.App.Sci

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Section: Resultssupporting

confidence: 83%

Section: Resultssupporting

confidence: 81%

Section: Resultsmentioning

confidence: 54%

Section: Resultsmentioning

confidence: 70%

See 2 more Smart Citations

Prevalence of Multidrug Resistant Uropathogenic E. coli: Role of β-lactamase

Gouda¹,

Zeid²

2017

Int.J.Curr.Microbiol.App.Sci

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“…22,23 In current study 15.90% Enterococci were isolated, found to be second most common organism causing UTI after E. coli. Among these enterococci, 69.09% and 76% of them were found resistant to levofloxacin and doxycycline respectively which are commonly and frequently prescribed drugs for UTI.…”

Section: Discussionmentioning

confidence: 99%

Recent pattern of drug sensitivity of most commonly isolated uropathogens from Central India

Lawhale¹,

Naikwade

2017

Int J Res Med Sci

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INTRODUCTIONUrinary tract infection (UTI) is one of the most common bacterial infection in the human population affecting all the age groups.1 It remains a major public health problem in terms of morbidity and financial cost. Worldwide about 150 million people are being diagnosed with urinary tract infection every year.2 Studies suggest that the overall prevalence of UTI is higher in women, an estimated 50% of women experience at least one episode of UTI at some point of their lifetime and between 20% and 40% of women can have recurrent episodes.3,4 UTI is less common in males as compared to females. 4 Urinary tract infection is due to an inflammatory response of urothelium to the invading pathogenic organisms.2 E-coli is the most common bacteria associated with the etiology of UTI. Other organisms ABSTRACT Background:The objective of the study is to assess the latest antibiotic sensitivity pattern of most commonly isolated organisms in urinary tract infection (UTI). Widespread and irrational use of antibiotics has led to development of highly resistant microorganisms. As the antibiotic sensitivity patterns of the microorganisms are frequently changing, this study was performed to assess the recent antibiotic sensitivity pattern in urinary tract infection among the human population. Methods: The study conducted from July 2016 to April 2017, in clinical microbiology laboratory, Sara Diagnostics (fully automated) laboratories, Nagpur, Maharashtra, India. 3000 urine samples were studied. The processing of the samples were done by standard microbiological methods. The antibiotic susceptibility was measured by disk diffusion test. CLSI guidelines were used for the antibiotic susceptibility evaluation. Results: Out of these, 1729 (57.63%) samples were culture positive showing significant bacteriuria. The most common bacteria isolated was E. coli (n=1040, 60.15%), followed by Enterococcus spp. (n=275, 15.90%), Klebsiella pneumoniae (n=210, 12.14%), Staphylococcus aureus (n=105, 6.07%) and Pseudomonas spp. (n=50, 2.89%) in this study. The study showed marked resistance to doxycycline, quinolones, cephalosporins and greater sensitivity to nitrofurantoin and fosfomycin, showing them better treatment options. Conclusions: The study confirmed, E.coli is still the most common bacteria to cause UTI, irrespective of geographical area. Nitrofurantoin, fosfomycin showed very high susceptibility to urinary tract infection pathogens, which include most commonly isolated gram-negative bacteria and gram-positive cocci, which were resistant to other various antibiotic groups. Thus, nitrofurantoin and fosfomycin seemed to be promising drugs.

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Cutaneous Vasculitis and Generalized Lymphadenopathy Associated with Extended-Spectrum Beta-Lactamase (ESBL)-Producing Escherichia coli Endocarditis: A Rare Case Report

et al. 2020

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Introduction Infective endocarditis (IE) has been known as the great imitator due to variable clinical manifestation, making its diagnosis challenging. A missed diagnosis could lead to inappropriate therapy. We presented a rare case of blood culture-negative infective endocarditis (BCNIE) due to extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli manifest with cutaneous vasculitis and generalized lymphadenopathy. We highlighted its diagnostic challenge and management. Case Illustration and Discussion A 36-year-old male with known asymptomatic ruptured sinus of Valsalva (SOV) presented with fever of unknown origin for six months, fatigue, weight loss with a history of multiple hospitalizations. The physical examination revealed a continuous murmur at Erb's point, cutaneous vasculitis, and bilateral inguinal lymphadenopathy. The laboratory result was leukocytosis and elevated C-Reactive Protein (CRP). Generalized lymphadenopathy was detected from the thorax and abdominal Computed Tomography (CT) Scans. Positive Anti Nuclear Antibody (ANA) Indirect Immunofluorescence (IF) speckled pattern led us to consider an autoimmune as the etiology, but we still considered IE as a differential diagnosis due to history of structural heart disease. Detection of multiple tiny oscillating masses at the tricuspid valve from the echocardiogram and cardiac CT led to possible IE diagnosis. Negative three consecutive blood cultures led the diagnosis to BCNIE. Surgery was performed to evacuate the vegetations, repair the SOV, and tricuspid valve replacement with a bioprosthetic valve. These results in improvement of the patient’s condition. ESBL-producing Escherichia coli yielded in tissue culture made the diagnosis of IE became definite. Conclusion ESBL-producing Escherichia coli should be considered as the etiology of BCNIE. Cutaneous vasculitis and generalized lymphadenopathy as a manifestation of IE could lead to diagnostic confusion. A thorough investigation will help clinician to avoid delay or inappropriate treatment that could be detrimental for the patient.

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Determination of Extended-Spectrum β-Lactamases and AmpC Production in Uropathogenic Isolates of Escherichia coli and Susceptibility to Fosfomycin

Cited by 40 publications

References 13 publications

Prevalence of Multidrug Resistant Uropathogenic E. coli: Role of β-lactamase

Prevalence of Multidrug Resistant Uropathogenic E. coli: Role of β-lactamase

Recent pattern of drug sensitivity of most commonly isolated uropathogens from Central India

Cutaneous Vasculitis and Generalized Lymphadenopathy Associated with Extended-Spectrum Beta-Lactamase (ESBL)-Producing Escherichia coli Endocarditis: A Rare Case Report

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