Determination of ethyl glucuronide in urine using reversed-phase HPLC and pulsed electrochemical detection (Part II)

Kaushik, Romina; LaCourse, William R.; Levine, Barry

doi:10.1016/j.aca.2005.08.060

Cited by 19 publications

(10 citation statements)

References 20 publications

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“…In their paper, the extraction step is a Solid Phase Extraction (SPE) of about 45 min. A similar extraction process was described by Shah and LaCourse (2006) and Kaushik et al (2006). This kind of extraction is more laborious than the one described in our paper, time consuming and larger amounts of solvent are needed.…”

Section: Resultsmentioning

confidence: 79%

Microwave assisted extraction for the determination of ethyl glucuronide in urine by gas chromatography‐mass spectrometry

Freire¹,

Barrera²,

Cid‐Silva³

et al. 2008

J of Applied Toxicology

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Alcohol is the most frequently abused 'addictive substance' that causes serious social problems throughout the world; thus alcoholism is of particular interest in clinical and forensic medicine. Ethyl glucuronide (EtG) is a marker of recent alcohol consumption that detects alcohol use reliably over a definite time period. The present paper describes a new method for the determination of EtG in urine. It was based both on microwave assisted extraction (MAE) to extract the analyte from urine samples, and gas chromatography-mass spectrometry (GC-MS) to identify and quantify the EtG in selected ion monitoring (SIM) mode. The method was applied to 33 urine samples from alcohol users, obtaining positive results in all cases. It was fully validated including a linear range (0.1-100 microg ml(-1)) and the main precision parameters. In summary, the use of microwave assisted extraction turned out to be a substantially simpler, faster and more sensitive procedure than any other conventional sample preparations.

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Section: Resultsmentioning

confidence: 79%

Microwave assisted extraction for the determination of ethyl glucuronide in urine by gas chromatography‐mass spectrometry

Freire¹,

Barrera²,

Cid‐Silva³

et al. 2008

J of Applied Toxicology

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“…Analyses such as these are considered important in the interpretation of relevant forensic and clinical information such as route of drug administration, dose and time of exposure as well as the ability to differentiate between recent and chronic drug use [81]. Recently, PED following HPLC was applied for the determination of ethyl glucuronide, a biomarker produced in ethanol consumption, in post-mortem urine samples [82]. Limits of detection were determined to be 30 ppb and repeatability at the limit of quantitation was reported as 1.7% RSD.…”

Section: Pharmaceutical and Toxicological Applicationsmentioning

confidence: 99%

A review of pulsed electrochemical detection following liquid chromatography and capillary electrophoresis

Fedorowski

LaCourse

2015

Analytica Chimica Acta

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“…However, alkyl-glucuronides such as EtG only adsorb at low UV wavelengths, and hence have little analytical utility. In light of this a method for the determination of EtG has been recently developed [44] using reversed-phase liquid chromatography coupled with pulsed electrochemical detection (PED) [45]. EtG was quantified using methyl glucuronide as an internal standard, and was separated using a mobile phase consisting of 1% acetic acid/acetonitrile (98/2, v/v).…”

Section: Ethanolmentioning

confidence: 99%

Review: The Application of Liquid Chromatography Electrochemical Detection for the Determination of Drugs of Abuse

Honeychurch

2016

Separations

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This review (4 tables, 88 references) describes current developments in the design and application of liquid chromatography electrochemical detection (LC ED) based approaches for the determination of drugs of abuse. Specific emphasis is placed on operating details and performance characteristics for selected applications. LC ED has been shown to be highly sensitive and specific as well being a more economic option. A wide range of abused substances have been determined using this approach, including: cannabinoids, ethanol, opiates, morphine, mushroom toxins, benzodiazepines and several legal highs. Reverse-phase liquid chromatography with either amperometric or coulometric determination has been the most commonly reported applications. However, coulometric arrays have been also reported. Detection limits in the ng/mL region have been reported for most target analytes.

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Determination of ethyl glucuronide in urine using reversed-phase HPLC and pulsed electrochemical detection (Part II)

Cited by 19 publications

References 20 publications

Microwave assisted extraction for the determination of ethyl glucuronide in urine by gas chromatography‐mass spectrometry

Microwave assisted extraction for the determination of ethyl glucuronide in urine by gas chromatography‐mass spectrometry

A review of pulsed electrochemical detection following liquid chromatography and capillary electrophoresis

Review: The Application of Liquid Chromatography Electrochemical Detection for the Determination of Drugs of Abuse

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