Determination of ethyl glucuronide in human serum by hyphenation of capillary isotachophoresis and zone electrophoresis

Nováková, Michaela; Křivánková, Ludmila

doi:10.1002/elps.200700663

Cited by 25 publications

(11 citation statements)

References 40 publications

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“…For the analysis of EtG in urine and serum, methods based on GC-MS [14 -16], LC-MS [1, 2, 5, 12, 15 -19] and ELISA [20,21] have been developed. Furthermore, CZE [22,23] and ITP-CZE [24] were applied to the determination of EtG in serum, and a commercial enzyme immunoassay (EIA) for urinary EtG became recently available and was found to be suitable for rapid urine screening [3,25,26]. The literature suggests that the calibration range for EtG in serum has to be in the low mg/L range.…”

Section: Introductionmentioning

confidence: 99%

Determination of ethyl glucuronide in human serum by capillary zone electrophoresis and an immunoassay

Jung

Caslavska

Thormann

2009

J of Separation Science

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Ethyl glucuronide (EtG) is a marker of recent alcohol consumption. For the optimization of the analysis of EtG by CZE with indirect absorbance detection, the use of capillaries with permanent and dynamic wall coatings, the composition of the BGE, and various sample preparation procedures, including dilution with water, ultrafiltration, protein precipitation, and SPE, were investigated. Two validated screening assays for the determination of EtG in human serum, a CZE-based approach and an enzyme immunoassay (EIA), are described. The CZE assay uses a coated capillary, 2,4-dimethylglutaric acid as an internal standard, and a pH 4.65 BGE comprising 9 mM nicotinic acid, epsilon-aminocaproic acid and 10% v/v ACN. Proteins are removed via precipitation with ACN prior to analysis and the LOQ is 0.50 mg/L. The EIA is based upon commercial reagents which are promoted for the determination of urinary EtG. Krebs-Ringer solution containing 5% BSA is used as a calibration matrix. All samples are ultrafiltered prior to analysis of the ultrafiltrate on a Mira Plus analyzer. Assay calibration ranged between 0 and 2 mg/L and the upper reference limit was determined to be 0.05 mg/L. Both assays proved to be suitable for the analysis of samples from different individuals. For EtG levels above 0.50 mg/L, good agreement was observed for the comparison of the results of the two methods.

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Section: Introductionmentioning

confidence: 99%

Determination of ethyl glucuronide in human serum by capillary zone electrophoresis and an immunoassay

Jung

Caslavska

Thormann

2009

J of Separation Science

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“…The literature suggests that the calibration range for EtG, for monitoring alcohol abuses, has to be in the low mg/L range. Three papers from the group of Thormann 33–35 have described the optimization and application of a CZE method based on separation in a pH 4.4 nicotinic acid/epsilon‐aminocaproic acid BGE in polyacrylamide‐coated capillaries with reversed polarity and indirect detection. Because of a transient isotachophoretic phenomenon, producing a sample self‐stacking effect, a sensitivity of 0.1 μg/mL for EtG was initially achieved and later improved to 2.2 ng/mL.…”

Section: Ions and Small Molecules Of Forensic Interestmentioning

confidence: 99%

Recent advances in the application of CE to forensic sciences, an update over years 2009–2011

2011

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The present article reviews and comments the applications of capillary electrophoresis in the different areas of forensic sciences covering the time from the second half of 2009 until the first part of 2011, being the latest update of previous reviews covering the years from 2001 to 2009. Numerous articles reporting applications of capillary electrophoresis to analytical problems of potential interest for the forensic researchers and scientists can be found in the most qualified journals of analytical chemistry, analytical biochemistry, pharmacology, toxicology, laboratory medicine, human genetics, etc. However, the present review has been focused on discussing only the most relevant examples of analytical applications of capillary electrophoretic and electrokinetic techniques published in the following fields: (i) illicit and abused drugs, (ii) ions and small molecules of forensic interest, (iii) proteins and peptides of forensic interest, (iv) dyes and inks, (v) forensic DNA. The present review collects and comments on 60 references.

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“…EtG has been measured in whole-blood and serum specimens by methods based on gas chromatography (GC) and liquid chromatography (LC) combined with MS, and by capillary electrophoresis [15][16][17][18][19][20][21][22], using solid-phase extraction or protein precipitation for sample preparation. Currently, LC-MS is mainly used as it allows EtG to be analyzed in its native form [16].…”

Section: Introductionmentioning

confidence: 99%

Sensitive determination of ethyl glucuronide in serum and whole blood: detection time after alcohol exposure compared with urine

Neumann

Beck

Helander

et al. 2020

Journal of Laboratory Medicine

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AbstractObjectivesEthyl glucuronide (EtG) is a conjugated, minor ethanol metabolite used as a biomarker for recent alcohol intake. EtG is commonly measured in urine as part of a drug testing service but has also attracted attention for measurement in blood. However, due to lower EtG concentrations in blood, the detection time is expected to be shorter. The present work aimed to improve the analytical sensitivity of EtG in blood, to prolong the detection time.MethodsA liquid chromatography-tandem mass spectrometry method was developed for EtG in whole blood and serum, using protein precipitation with methanol, a deuterated internal standard, and selected reaction monitoring mode with negative electrospray ionization. No significant matrix effect was observed. The method generated linear results in the measuring range 1.0–50 μg/L, the accuracy was within ±10% and the imprecision <15%.ResultsIn 46 patients followed with daily blood and urine sampling during alcohol detoxification, the mean (median) time to first negative serum EtG sample was 112 (111) h. This was slightly longer than for EtG in urine, using 100 μg/L as cutoff. The detection rate was 76% for serum EtG and 68% for urine EtG. In cases where serum EtG was positive but urine EtG negative, the urine samples tended to be more dilute as indicated by lower creatinine concentrations. On admission to hospital, the whole-blood and serum EtG concentrations correlated with the breath ethanol concentration (p = 0.012 and p = 0.027, respectively). In 100 patients sampled at admission to hospital for other reasons than substance abuse and with no ethanol detected in breath, 40% tested positive for EtG in serum and 43% in urine. In 79 paired urine and serum EtG measurements, the median urine/serum concentration ratio was 155.ConclusionsA sensitive method was developed for EtG measurement in whole-blood and serum specimens, offering similar detection time for recent alcohol exposure compared with routine EtG measurement in urine.

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Determination of ethyl glucuronide in human serum by hyphenation of capillary isotachophoresis and zone electrophoresis

Cited by 25 publications

References 40 publications

Determination of ethyl glucuronide in human serum by capillary zone electrophoresis and an immunoassay

Determination of ethyl glucuronide in human serum by capillary zone electrophoresis and an immunoassay

Recent advances in the application of CE to forensic sciences, an update over years 2009–2011

Sensitive determination of ethyl glucuronide in serum and whole blood: detection time after alcohol exposure compared with urine

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