Determination of estrogens in river water by gas chromatography–negative-ion chemical-ionization mass spectrometry

Nakamura, Sadao; Sian, Tan Hwee; Daishima, Shigeki

doi:10.1016/s0021-9673(01)00851-2

Cited by 74 publications

(37 citation statements)

References 14 publications

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“…Besides therapeutic administration, lymphocytes can be exposed to high levels of 17β-estradiol. 17β-Estradiol from river water 28,29) might be biologically concentrated through the food chain and consumed by humans. Therefore, it is possible that human lymphocytes are exposed to high levels of estrogenic compounds and lymphocyte mitogenesis is suppressed.…”

Section: Resultsmentioning

confidence: 99%

Apoptosis Induction of Mouse Splenic Cells by Exposure to High-Level 17.BETA.-Estradiol and Endocrine Disrupting Chemicals

Sakazaki

Ueno

Nakamuro

2004

JOURNAL OF HEALTH SCIENCE

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Section: Resultsmentioning

confidence: 99%

Apoptosis Induction of Mouse Splenic Cells by Exposure to High-Level 17.BETA.-Estradiol and Endocrine Disrupting Chemicals

Sakazaki

Ueno

Nakamuro

2004

JOURNAL OF HEALTH SCIENCE

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“…All samples were derivatized with PFBBr following Nakamura et al [20]. The 1 mL acetone solutions were treated with 100 lL of 10% aqueous potassium carbonate solution and subsequently with 100 lL of 5% PFBBr in acetone.…”

Section: Derivatization With Pentafluorobenzyl Bromidementioning

confidence: 99%

Determination of Endocrine Disrupting Compounds and Acidic Drugs in Water by Coupling of Derivatization, Gas Chromatography and Negative‐Chemical Ionization Mass Spectrometry

Möder¹,

Braun

Lange

et al. 2007

CLEAN Soil Air Water

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The determination of acidic pharmaceutical and endocrine disrupting compounds at low ng/L levels in surface and wastewater requires highly selective and sensitive analytical procedures. Therefore, the water samples under study were prepared by means of solid phase extraction (SPE) for analyte enrichment and clean up. Prior to GC-NCI-MS, the polar analytes were derivatized using pentafluorobenzyl bromide. The performance of this analytical method has been revealed in fortification experiments by limits of detection ranging from 0.01 to 0.2 ng/L for, e. g., ibuprofen and 17a-ethinylestradiol, respectively. SPE may be the most rate determining step of the analytical procedure. Therefore, the performance of SPE disks and cartridges was additionally compared. Method evaluation demonstrated that even complex sample matrices, such as model wastewater or even synthetic humic acids, did not interfere with the quantification and identification of target analytes. The performance of the analytical method developed for water monitoring was further emphasized by the investigation of surface water of the River Saale and effluents of the wastewater treatment plant (WWTP) at Halle, Saxony-Anhalt, Germany. In those samples, acidic pharmaceuticals and corresponding metabolites occurred at 0.1 ng/L for clofibric acid up to 498 ng/L for bezafibrate. Technical nonylphenol and bisphenol A were found in every water sample. Meanwhile, 17a-ethinylestradiol was determined only in one WWTP effluent sample at 1 ng/L.

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“…The derivatisation was carried out using a modified version of the method used by Nakamura et al (2001) for the pentafluorobenzyl-trimethylsilyl derivative. To the acetone extract, 100 µL of 10% aqueous potassium carbonate and 10 µL of pentafluorobenzylbromide reagent were added, and were kept at 70 0 C for 1 hour.…”

Section: Sample Derivatisation For Gc-ms Analysismentioning

confidence: 99%

Steroid estrogens in ocean sediments

et al. 2005

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Ocean sediment samples were taken adjacent and 7km from a deep ocean outfall (average depth 80m), the discharge point of an enhanced primary sewage treatment plant. All samples contained steroid estrogens at nanogram per gram levels with higher concentrations at the 7km sampling site. The concentration of estrone ranged from (0.16 -1.17 ng/g), 17β-estradiol (0.22 -2.48 ng/g) and the synthetic 17α-ethinylestradiol (<0.1 -0.5 ng/g). The values detected correspond with estimates based on the proportion of estrogens sorbed to particles in the effluent and the expected proportion of sewage origin particles in the ocean sediments. The results suggest that estrogens associated with the particulate fraction aggregate on contact with high ionic strength seawater and settle to the seafloor after discharge through deep ocean outfalls.

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Determination of estrogens in river water by gas chromatography–negative-ion chemical-ionization mass spectrometry

Cited by 74 publications

References 14 publications

Apoptosis Induction of Mouse Splenic Cells by Exposure to High-Level 17.BETA.-Estradiol and Endocrine Disrupting Chemicals

Apoptosis Induction of Mouse Splenic Cells by Exposure to High-Level 17.BETA.-Estradiol and Endocrine Disrupting Chemicals

Determination of Endocrine Disrupting Compounds and Acidic Drugs in Water by Coupling of Derivatization, Gas Chromatography and Negative‐Chemical Ionization Mass Spectrometry

Steroid estrogens in ocean sediments

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