Determination of Estrogens in Raw and Treated Wastewater by High-Performance Liquid Chromatography-Ultraviolet Detection

Fonseca, Ana Paula; Cardoso, Salvador Massano; Esteves, Valdemar I.

doi:10.4172/2161-0525.1000203

Cited by 9 publications

(12 citation statements)

References 23 publications

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“…The MDLs calculated for the target compounds ranged from 0.18 ng·mL −1 for 17␤-estradiol-3 methyl ether to 0.45 ng·mL −1 for 17␣-ethynylestradiol. These limits are slightly better to those found in other studies that determined estrogens by LC or UHPLC with an optical detector and traditional sorbents in SPE [28][29][30]. In Table 4 it is shown that a comparison with other chromatographic methods based on SPE using imprinted polymers.…”

Section: Analytical Parametersmentioning

confidence: 58%

Molecularly imprinted solid-phase extraction coupled with ultra high performance liquid chromatography and fluorescence detection for the determination of estrogens and their metabolites in wastewater

et al. 2015

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Estrogens are an important class of endocrine-disrupting compounds, and their contamination of environmental waters through the effluents of wastewater treatment plants could have an important impact on aquatic biota, even at low concentrations. For this reason, the development of selective and sensitive extraction methodologies, which permit the identification and quantification of these compounds at trace level concentrations, is very important. In this study, a quantitative method based on molecularly imprinted solid phase extraction coupled to ultra high performance liquid chromatography with fluorescence detection has been developed. It has been used for the simultaneous determination of three estrogens and two of their metabolites in water samples from wastewater treatment plants. The method developed presents satisfactory limits of detection (between 0.18 and 0.45 ng·mL ), good recoveries (higher than 60%) and low relative standard deviations (under 10%). The method was used to analyze wastewater from a veterinary hospital as well as influent and effluent samples of a wastewater treatment plant of Gran Canaria (Spain) The concentrations of the detected hormones ranged from 1.35 to 2.57 ng·mL .

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Section: Analytical Parametersmentioning

confidence: 58%

Molecularly imprinted solid-phase extraction coupled with ultra high performance liquid chromatography and fluorescence detection for the determination of estrogens and their metabolites in wastewater

et al. 2015

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“…The highest SMA was obtained in H 2 /CO 2 (515 ± 52 mLCH 4 @SPT/gVS.day) assays, and in acetate and VFA, the SMA was 79 ± 11 mLCH 4 @SPT/gVS.day and 82 ± 13 mLCH 4 @SPT/gVS.day, respectively. In the assays performed with pharmaceuticals, no inhibition associated with the presence of pharmaceuticals was observed at the minimal concentration assessed (0.01 mg/L) (Table 2), which is the closest concentration to the ones found in real WWTP (Fonseca et al 2013;Pereira et al 2015;Sousa 2015). The SMA was also not affected by any of the tested pharmaceuticals at concentrations up to 0.1 mg/L (Table 2), which is higher than the concentrations found in WWTP (Fonseca et al 2013;Pereira et al 2015;Sousa 2015).…”

Section: Resultsmentioning

confidence: 74%

“…Although pharmaceuticals are usually detected in wastewater in relatively low concentrations (in the range of ng/L to µg/L) (Jones et al 2005;Quintana et al 2005;Liebig et al 2006;Radjenovic et al 2007;Scheurell et al 2009;Jelic et al 2011;Gonzalez-Gil et al 2016;Subedi and Loganathan 2016), higher levels (14 to 31 mg/L of ciprofloxacin (CIP)) have already been detected in the effluents of wastewater treatment plants (WWTP) in India (Larsson et al 2007;Fick et al 2009) as well as in the surface water from two lakes in India that were not contaminated by the WWTP, and in wells of six nearby villages (Fick et al 2009). In Portugal, 17.5 µg/L of CIP (Pereira et al 2015), 2.4 µg/L of ibuprofen (IBP) (Pereira et al 2015;Sousa 2015), 8.6 µg/L of diclofenac (DCF) (Pereira et al 2015;Sousa 2015) and 0.19 µg/L of 17 α-ethinylestradiol (EE2) (Fonseca et al 2013), are usually found in wastewater (WWTP influents). However, the concentration of pharmaceutical compounds adsorbed in sewage sludge can be much higher.…”

Section: Introductionmentioning

confidence: 99%

Ciprofloxacin, diclofenac, ibuprofen and 17α-ethinylestradiol differentially affect the activity of acetogens and methanogens in anaerobic communities

et al. 2020

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Pharmaceutical compounds end up in wastewater treatment plants but little is known on their effect towards the different microbial groups in anaerobic communities. In this work, the effect of the antibiotic Ciprofloxacin (CIP), the non-steroidal anti-inflammatory drugs Diclofenac (DCF) and Ibuprofen (IBP), and the hormone 17α-ethinylestradiol (EE2), on the activity of acetogens and methanogens in anaerobic communities, was investigated. Microbial communities were more affected by CIP, followed by EE2, DCF and IBP, but the response of the different microbial groups was dissimilar. For concentrations of 0.01 to 0.1 mg/L, the specific methanogenic activity was not affected. Acetogenic bacteria were sensitive to CIP concentrations above 1 mg/L, while DCF and EE2 toxicity was only detected for concentrations higher than 10 mg/L, and IBP had no effect in all concentrations tested. Acetoclastic methanogens showed higher sensitivity to the presence of these micropollutants, being affect by all the tested pharmaceutical compounds although at different degrees. Hydrogenotrophic methanogens were not affected by any concentration, indicating their lower sensitivity to these compounds when compared to acetoclasts and acetogens.

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“…A relevância dos hormônios estrogênicos na perturbação endócrina se deve: à alta afinidade dos estrógenos aos receptores presentes nos organismos de outras espécies permitindo a ação, mesmo em baixíssimas concentrações (ng L -1 ); à ampla excreção destes hormônios por seres humanos e por outros animais nas fezes e urina; e ao posterior despejo dos esgotos, tratados ou não, nos ecossistemas aquáticos (Carballa et al, 2004;Fonseca et al, 2013).…”

Section: Introductionunclassified

“…Já os hormônios artificiais têm sido determinados menos frequentemente, visto que a sua presença depende da prescrição e regulamentação para uso (Carballa et al, 2004;Janex-Habibi et al, 2009;Stanford e Weinberg, 2010;Fonseca et al, 2013;Ferreira, 2013).…”

Section: Introductionunclassified

Determinação de hormônios estrogênicos em esgoto bruto e efluente de uma estação descentralizada de tratamento por lodos ativados

Teixeira¹,

Marques²,

Carvalho³

et al. 2018

Rev. ambiente água

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RESUMOHormônios estrogênicos, provenientes dos esgotos, atingem os corpos hídricos e podem causar perturbações aos organismos aquáticos. Os tratamentos secundários das estações de tratamento de esgoto (ETE) possibilitam alguma remoção destes compostos, mesmo em ETEs descentralizadas, apesar de ainda haver poucos estudos sobre elas. Em sistemas por lodos ativados, a eficiência tem sido relacionada ao tempo de detenção hidráulica (TDH), à idade de lodo, à desnitrificação biológica e à carga de alimentação. O objetivo deste trabalho foi quantificar os hormônios estrona (E1), 17-β-estradiol (E2), estriol (E3) e 17-α-etinilestradiol (EE2) no esgoto e no efluente de uma ETE descentralizada por lodos ativados, além de caracterizar a ETE do Campus Universitário da EEL-USP quanto à matéria orgânica recebida, ao tempo de detenção hidráulica e a remoção de nitrogênio. Cromatografia líquida com detecção UV foi utilizada na determinação dos hormônios. Os resultados mostraram concentrações dos hormônios superiores às encontradas na literatura, em ambos: esgoto (5,158 ± 2,747; 7,434 ± 4,356; 5,200 ± 3,331 e 5,638 ± 4,312 μg L -1 de E1, E2, E3 e EE2) e efluente (5,062 ± 3,366; 4,191 ± 3,527; 7,743 ± 3,951, 2,550± 2,162 de E1, E2, E3 e EE2). No esgoto, as altas concentrações podem ser relacionadas à maior predominância de urina, visto os altos níveis de nitrogênio detectados, à menor geração de esgoto e a amostragem em período seco causando reduzida diluição dos hormônios. Remoção insuficiente dos hormônios devido a menor TDH (2h05) e irregularidade na desnitrificação (-54 a 61%) pode ser o motivo das altas concentrações de estrógenos no efluente. Ademais, a desconjugação dos hormônios pode ter ocorrido durante o tratamento.Palavras-chave: cromatografia líquida com detecção UV, esgoto de Campus universitário, estrógenos, ETE batelada, remoção de hormônios estrogênicos.Determination of estrogenic hormones in sewage and effluent of a decentralized sewage treatment plant by activated sludge ABSTRACT Estrogenic hormones from sewers reach water bodies and can disrupt aquatic organisms. Secondary treatment of sewage treatment plants (STP) can remove some of those hormones,

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Determination of Estrogens in Raw and Treated Wastewater by High-Performance Liquid Chromatography-Ultraviolet Detection

Cited by 9 publications

References 23 publications

Molecularly imprinted solid-phase extraction coupled with ultra high performance liquid chromatography and fluorescence detection for the determination of estrogens and their metabolites in wastewater

Molecularly imprinted solid-phase extraction coupled with ultra high performance liquid chromatography and fluorescence detection for the determination of estrogens and their metabolites in wastewater

Ciprofloxacin, diclofenac, ibuprofen and 17α-ethinylestradiol differentially affect the activity of acetogens and methanogens in anaerobic communities

Determinação de hormônios estrogênicos em esgoto bruto e efluente de uma estação descentralizada de tratamento por lodos ativados

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