Determination of Estrogens in Low and High Titer Urines Using Thin Layer and Gas Liquid Chromatography.

Wotiz, Herbert H.; Chattoraj, Sati C.

doi:10.1021/ac60214a015

Cited by 54 publications

(7 citation statements)

References 30 publications

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“…Free sterols were converted to acetates according to Wotiz and Chattoraj (1964). G C was performed on a Shimadzu GC-R1A gas chromatograph equipped with a FID.…”

Section: Acetylationmentioning

confidence: 99%

Chemical composition of tobacco seeds (Nicotiana tabacum L) from Argentina

Maestri

Guzmán

1993

J Sci Food Agric

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Abstract:The lipid constituents, free fatty acids, triglycerides, sterol esters and free sterols of 11 cultivars of tobacco from Argentina were investigated by gas-liquid chromatography. Palmitic, oleic and linoleic acids were the major components in all fractions studied. Sitosterol was the main component of the free sterol and sterol ester fractions. Oil and protein contents, as well as some physicochemical characteristics of the oils, are also reported.

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“…Free sterols were converted to acetates according to Wotiz and Chattoraj (1964). G C was performed on a Shimadzu GC-R1A gas chromatograph equipped with a FID.…”

Section: Acetylationmentioning

confidence: 99%

Chemical composition of tobacco seeds (Nicotiana tabacum L) from Argentina

Maestri

Guzmán

1993

J Sci Food Agric

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“…The column packings were prepared by the method of Wotiz & Chattoraj (1964) and consisted of 3-8% SE-30 on silanized Diatoport S (60-80 mesh). After packing, the glass U-shaped columns (6 ft. by 4 mm.…”

Section: Gas-liquid Chromatography (Glc)mentioning

confidence: 99%

Adrenal Secretion Rates of C-19 and C-21 Steroids Before and After Hypophysectomy in the Pig and the Dog

Heap¹,

Holzbauer²,

Newport³

1966

Journal of Endocrinology

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1) The adrenal secretion of androgens and of some intermediates of corticosteroid biosynthesis have been studied in immature pigs and adult dogs under anaesthesia.(2) Qualitative and quantitative analyses of the extracts of adrenal venous blood were performed by a combination of paper and gas chromatographic techniques.(3) In both species 1 1\g=b\OH-androstenedione,pregnenolone, progesterone and 1 1\g=b\OH-progesterone were secreted under stress conditions at a mean rate of approximately 20 \g=m\g./adrenal/hr.; this was somewhat more than aldosterone secretion and about one-fiftieth of the glucocorticoid secretion.(4) Within 2 hr. of hypophysectomy the secretion of all four compounds was decreased by more than 95%.

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“…The acetylated oestrogen fractions were run on silica gel H in the system petroleum ether (40/60) :methanol, 9:1 (Wotiz & Chattoraj, 1964), the appropriate areas were eluted with methanol and rechromatographed on QF-1 and SE-30 against standard acetates. The retention times were unchanged.…”

mentioning

confidence: 99%