Determination of estrogenic activity in landfill leachate by simplified yeast two-hybrid assay

Kawagoshi, Yasunori; Tsukagoshi, Yukiko; Fukunaga, Isao

doi:10.1039/b206161a

Cited by 17 publications

(16 citation statements)

References 21 publications

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“…The yeast two-hybrid assay was performed according to the method of Kawagoshi et al (15,16). The yeast strain Saccharomyces cerevisiae Y190 was used.…”

Section: Yeast Two-hybrid Assaymentioning

confidence: 99%

Degradation and behavior of natural steroid hormones in cow manure waste during biological treatments and ozone oxidation

Ermawati

Morimura

Tang

et al. 2007

Journal of Bioscience and Bioengineering

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“…The yeast two-hybrid assay was performed according to the method of Kawagoshi et al (15,16). The yeast strain Saccharomyces cerevisiae Y190 was used.…”

Section: Yeast Two-hybrid Assaymentioning

confidence: 99%

Degradation and behavior of natural steroid hormones in cow manure waste during biological treatments and ozone oxidation

Ermawati

Morimura

Tang

et al. 2007

Journal of Bioscience and Bioengineering

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“…According to Masoner et al, landfill leachates can contain complex mixtures of CECs that originate from a variety of sources [ 49 ]. A study conducted by Kawagoshi et al, detected estrogenic activity in leachates from a municipal waste-dumping site in Japan [ 50 ].…”

Section: Discussionmentioning

confidence: 99%

Analysis of Estrogenic Activity in Maryland Coastal Bays Using the MCF-7 Cell Proliferation Assay

Elfadul

Jesien²,

Elnabawi

et al. 2021

IJERPH

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Contaminants of Emerging Concern (CECs) with estrogenic or estrogenic-like activity have been increasingly detected in aquatic environments and have been an issue of global concern due to their potential negative effects on wildlife and human health. This study used the MCF-7 cell proliferation assay (E-Screen) to assess the estrogenic activity profiles in Maryland Coastal Bays (MCBs), a eutrophic system of estuaries impacted by human activities. Estrogenic activity was observed in all study sites tested. Water samples from MCBs increased MCF-7 cell proliferation above the negative control from 2.1-fold at site 8, located in Sinepuxent Bay close to the Ocean City Inlet, to 6.3-fold at site 6, located in Newport Bay. The proliferative effects of the sediment samples over the negative control ranged from 1.9-fold at the Assateague Island National Seashore site to 7.7-fold at the Public Landing site. Moreover, elevated cell proliferation (p < 0.05) was observed when cells were co-exposed with 17ß-Estradiol (E2), while reduction in cell proliferation was observed when cells were co-exposed with the antagonist ICI 182, 780 suggesting that cell proliferative effects were primarily mediated by the estrogen receptor (ER). These results suggest the occurrence of some estrogenic or hormonal-like compounds in the MCBs and are consistent with our previous findings based on vitellogenin analyses.

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“…With a global production of almost 360 million metric tons in 2018 [ 7 ], manufacturing, use, and disposal of plastic materials pose major safety concerns. Leachate from landfills, migration from consumer products (e.g., food packaging, toys, flooring, textiles), and air pollution from burning plastic materials are just some of the sources of chemical contamination affecting humans and the environment [ 8 – 10 ] . Because information on chemicals present in plastics is difficult to obtain and their hazards often remain unknown, Groh and colleagues [ 11 ] published a comprehensive database with more than 900 chemicals likely associated with plastic packaging as part of the Hazardous Chemicals in Plastic Packaging (HCPP) project.…”

Section: Introductionmentioning

confidence: 99%

Role of epidemiology in risk assessment: a case study of five ortho-phthalates

et al. 2021

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Background The association between environmental chemical exposures and chronic diseases is of increasing concern. Chemical risk assessment relies heavily on pre-market toxicity testing to identify safe levels of exposure, often known as reference doses (RfD), expected to be protective of human health. Although some RfDs have been reassessed in light of new hazard information, it is not a common practice. Continuous surveillance of animal and human data, both in terms of exposures and associated health outcomes, could provide valuable information to risk assessors and regulators. Using ortho-phthalates as case study, we asked whether RfDs deduced from male reproductive toxicity studies and set by traditional regulatory toxicology approaches sufficiently protect the population for other health outcomes. Methods We searched for epidemiological studies on benzyl butyl phthalate (BBP), diisobutyl phthalate (DIBP), dibutyl phthalate (DBP), dicyclohexyl phthalate (DCHP), and bis(2-ethylhexyl) phthalate (DEHP). Data were extracted from studies where any of the five chemicals or their metabolites were measured and showed a statistically significant association with a health outcome; 38 studies met the criteria. We estimated intake for each phthalate from urinary metabolite concentration and compared estimated intake ranges associated with health endpoints to each phthalate’s RfD. Result For DBP, DIBP, and BBP, the estimated intake ranges significantly associated with health endpoints were all below their individual RfDs. For DEHP, the intake range included associations at levels both below and above its RfD. For DCHP, no relevant studies could be identified. The significantly affected endpoints revealed by our analysis include metabolic, neurodevelopmental and behavioral disorders, obesity, and changes in hormone levels. Most of these conditions are not routinely evaluated in animal testing employed in regulatory toxicology. Conclusion We conclude that for DBP, DIBP, BBP, and DEHP current RfDs estimated based on male reproductive toxicity may not be sufficiently protective of other health effects. Thus, a new approach is needed where post-market exposures, epidemiological and clinical data are systematically reviewed to ensure adequate health protection.

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Determination of estrogenic activity in landfill leachate by simplified yeast two-hybrid assay

Cited by 17 publications

References 21 publications

Degradation and behavior of natural steroid hormones in cow manure waste during biological treatments and ozone oxidation

Degradation and behavior of natural steroid hormones in cow manure waste during biological treatments and ozone oxidation

Analysis of Estrogenic Activity in Maryland Coastal Bays Using the MCF-7 Cell Proliferation Assay

Role of epidemiology in risk assessment: a case study of five ortho-phthalates

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