Determination of essential amino acids in human serum by a targeting method based on automated SPE–LC–MS/MS: Discrimination between artherosclerotic patients

Calderón-Santiago, M.; Priego-Capote, Feliciano; Galache-Osuna, J.G.; Castro, M.D. Luque de

doi:10.1016/j.jpba.2012.06.033

Cited by 27 publications

(22 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our findings revealed that AngSc is correlated with serum levels of phenylalanine and tyrosine. Accordingly, the results of animal experiments 28 and human studies 29 indicate that myocardial infarction is associated with increased levels of phenylalanine. It could be hypothesized that the association of phenylalanine with the extent of atherosclerosis is influenced by high-grade oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

Metabolomic signature of arterial stiffness in male patients with peripheral arterial disease

et al. 2015

View full text Add to dashboard Cite

Arterial stiffness is an independent determinant of cardiovascular risk and a marker of subclinical organ damage. Metabolomics may facilitate identification of novel low-molecular cardiovascular risk factors. The aim of the present study was to compare metabolic signatures and functional-biochemical characteristics of patients with peripheral arterial disease (PAD) and clinically healthy subjects. We studied 42 men with symptomatic PAD (aged 66±7 years) and 46 healthy men (aged 66±8 years). Aortic pulse wave velocity (aPWV) was assessed by applanation tonometry using the Sphygmocor device. Metabolic profiling was performed with high-performance liquid chromatography and mass spectrometry. Serum oxidized low-density lipoprotein (oxLDL) level was measured by enzyme-linked immunosorbent assay. The aPWV as well as serum levels of lactate, free carnitine and 11 amino acids including tyrosine were higher among the patients with PAD. In contrast, serum levels of pyruvate, citrate, α-ketoglutarate, aconitate and cysteine were higher in the control group. In multiple regression models, aPWV was independently determined by log-tyrosine and log-oxLDL in the patients (R(2)=0.61; P<0.001) and by age, log-pyruvate and log-oxLDL in the controls (R(2)=0.52; P<0.001). Our study describes for the first time significant differences in metabolomic signature of patients with advanced atherosclerosis compared with clinically healthy controls. The aPWV is independently associated with serum levels of tyrosine and oxLDL in the patients with PAD and is related to pyruvate and oxLDL levels in the control group. The measurement of low-molecular metabolites, which are related to changes in vascular phenotypes, may lead to identification of novel vascular risk markers.

show abstract

Section: Discussionmentioning

confidence: 99%

Metabolomic signature of arterial stiffness in male patients with peripheral arterial disease

et al. 2015

View full text Add to dashboard Cite

show abstract

“…On the other hand, amino acids are considered as a nutrient for human health and some of them are essential, such as lysine and phenylalanine. 18,19 The main advantage of taking amino acid profiles as variables is that the results can be used not only to discriminate the botanical origin, but also to determine the nutritional value of honey. From this perspective, the results of the present study could guide consumer's choices based on their different health needs.…”

Section: Introductionmentioning

confidence: 99%

Discrimination of botanical origins for Chinese honey according to free amino acids content by high‐performance liquid chromatography with fluorescence detection with chemometric approaches

et al. 2016

View full text Add to dashboard Cite

show abstract

“…However, factors that contributed to the differences in amino acid levels were ischemia and obesity rather than gender [43]. In this study, changes in amino acid levels were dependent on MI, not gender differences.…”

Section: Discussionmentioning

confidence: 51%

LC/MS-based polar metabolite profiling reveals gender differences in serum from patients with myocardial infarction

Lee

Jung

Park

et al. 2015

Journal of Pharmaceutical and Biomedical Analysis

View full text Add to dashboard Cite

Determination of essential amino acids in human serum by a targeting method based on automated SPE–LC–MS/MS: Discrimination between artherosclerotic patients

Cited by 27 publications

References 22 publications

Metabolomic signature of arterial stiffness in male patients with peripheral arterial disease

Metabolomic signature of arterial stiffness in male patients with peripheral arterial disease

Discrimination of botanical origins for Chinese honey according to free amino acids content by high‐performance liquid chromatography with fluorescence detection with chemometric approaches

LC/MS-based polar metabolite profiling reveals gender differences in serum from patients with myocardial infarction

Contact Info

Product

Resources

About