Determination of endotoxins in the vital pulp of human carious teeth: Association with pulpal pain

In vitro effects of calcium hydroxide and polymyxin B on endotoxins in root canals

“…Schein and Schilder, 3 Horiba et al 27 and Khabbaz et al 19 showed that symptomatic teeth present greater amount of endotoxin than asymptomatic teeth. Many dentists use the combination neomycin-polymyxin B-hydrocortisone to treat teeth with vital pulp due to its satisfactory anti-inflammatory action and capacity of preserving the periapical tissues.…”

“…Inflammatory reactions and bone resorption occurred in the periapical tissues of all the experiment teeth. Khabbaz et al 18,19 demonstrated that endotoxins are present in carious lesions of symptomatic and asymptomatic teeth and in the pulpal tissue of the carious teeth, indicating that LPS may play an important role in the pathogenesis of human pulpal diseases.…”

LPS levels in root canals after the use of ozone gas and high frequency electrical pulses

“…1,2,3 LPS is released during multiplication or death of gram-negative bacteria, and is associated with many biological effects, such as release of pro-inflammatory mediators 4 and induction of periapical bone resorption. 5 The endotoxins can adhere to mineralized tissues 6 and disseminate through the dentinal tubules, 7 making root canal sterilization difficult if only chemo-mechanical preparation is used.…”

Ação do laser de Nd:YAG intracanal sobre endotoxinas na dentina radicular

“…O experimento realizado analisou a atuação do laser em endotoxinas bacterianas em virtude do papel que desempenham localmente e à distância (DAHLEN;MAGNUSSON;MOLLER, 1981;DWYER;TORABINEJAD, 1981;HORIBA et al,1990;HORIBA et al, 1991;HORIBA et al,1992;HOSOYA;MATSUSHIMA, 1997;JACINTO et al, 2005;JIANG et al, 2002;SYKARAS, 2000;SYKARAS, 2001;MURRAY;SAUNDERS, 2000;NISSAN et al,1995;OLIVEIRA et al, 2005a;PITTS;WILLIAMS;MORTON, 1982;RAISZ et al, 1981;SCHEIN;SCHILDER, 1975;SCHONFELD et al, 1982;WESSELINK;THODEN VAN VELZEN;MAKKES, 1978;YAMASAKI et al, 1992; (BUCK et al 2001;OLIVEIRA et al, 2005b;OLIVEIRA et al 2007;NICHOLS 1993;NICHOLS 1994).…”

“…No entanto, a microbiota com polpa necrótica é dominada por bactérias anaeróbicas, das quais, usualmente, apenas de uma até 12 espécies de bactérias são encontradas no sistema de canais radiculares (FABRICIUS et al, 1982;SUNDQVIST, 1992;ØRSTAVIK;FORD, 1998 (GOMES et al, 2004;LILLEY;DRUCKER, 1996;JACINTO et al, 2003;FARBER, 1994;SUNDQVIST, 1992 JIANG et al, 2002;PITTS;WILLIAMS;MORTON, 1982;RAISZ et al, 1981;WESSELINK;THODEN VAN VELZEN;MAKKES, 1978), estão associadas a canais radiculares com sintomas clínicos e áreas periapicais radiolucentes (HORIBA et al, 1991;JACINTO et al, 2005;SYKARAS, 2000;SYKARAS, 2001;SCHEIN;SCHILDER, 1975;SCHONFELD et al, 1982;YAMASAKI et al, 1992), promovem a ativação do sistema complemento ) e possuem habilidade de se difundirem através da dentina humana (HORIBA et al,1990;NISSAN et al,1995;OLIVEIRA et al, 2005a).…”