Determination of Endogenous Hormones Content in Cotton Varieties (Gossypiumhirsutum) as Influenced by Phosphorus and Potassium Nutrition

Abstract

Two experiments were conducted to evaluate the endogenous hormones content of cotton plants grown hydroponically, and supplied with varying levels of phosphorus (P) and potassium (K) mineral nutrients in the first cropping. In the second cropping, varying levels of exogenous indole-3-acetic acid (IAA), gibberellic acid (GA 3 ) and zeatin (Z) and their combinations were applied to high level of PK in the hydroponic. In the first experiment, low K and low P treated plants had higher content of gibberellic acid (GA 3 ) in the leaves than high PK treated plants, and low K treated plants also produced more Zeatin (Z) in the leaves than either low P or high PK treated plants. Furthermore, low K treated plants had higher Zeatin (Z) content in the root than either low P or high PK treated plants. However, the two cotton varieties expressed different hormones content in the leaves, stem and root. In the second experiment, GA 3 concentration was highest in the cotton stem treated to IAA x GA 3 x Z. Moreover, Zeatin content in the leaves, stem and root was highest in the cotton treated to IAA x GA 3 x Z and 2IAA x 2GA 3 x 2Z for leaves, IAA x GA 3 x Z for stem and Z, IAA x 2GA 3 x Z for root. Nevertheless, two cotton varieties possessed different hormones content in the leaves, stem and root. In general, there was no relationship between the first cropping and second cropping. The results demonstrated influence of exogenous hormones application in altering the endogenous hormones content of cotton plants.

Screening and identification of miRNAs related to sexual differentiation of strobili in Ginkgo biloba by integration analysis of small RNA, RNA, and degradome sequencing

“…Added equal volume of ethyl acetate for extraction for 3 times, discard the water phase, concentrate the remaining organic phase to about 2 mL using a rotary evaporator under reduced pressure at 35°C, dissolved with methanol (chromatographic grade), and fixed the volume to 10 mL, then filtered with a 0.45 μm filter membrane to obtain the sample solution. High performance liquid chromatography (HPLC, Thermo Scientific™ UltiMate™ 3000, USA) with an Agilent C-18 chromatographic column (5 μm, 4.6 mm × 250 mm) was used for quantitative analysis of IAA, GA3 and ZT [65] with following modification. The mobile phase was methanol-1% acetic acid (40:60); flow rate: 0.6 mL/min; column temperature: 35°C; injection volume: 2 μL; detection at 269 nm.…”

Screening and identification of miRNAs related to sexual differentiation of strobili in Ginkgo biloba by integration analysis of small RNA, mRNA, and degradome sequencing

“…The extraction and content determination of IAA, GA 3 and ZT according to the method described by Onanuga et al [64] with following modi cation. The preparation sample was as below: 5 g of fresh sample was ground into powder 4 °C, and then added 50 ml of precooled 80% methanol solution.…”