Determination of endogenous and exogenous corticosteroids in bovine urine and effect of fighting stress during the “Batailles des Reines” on their biosynthesis

Leporati, Marta; Nobile, Maria; Capra, P; Alladio, Eugenio; Vincenti, Marco

doi:10.1016/j.rvsc.2017.06.014

Cited by 4 publications

(1 citation statement)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The detected prednisolone levels were found much below the official cut off of 5.0 μg L − 1 established to avoid false non-compliances. Based on the results of this and other studies [11, 14], it may also be concluded that, in cows subjected to various natural sources of stress, the occasional positive finding of urinary endogenous prednisolone is never matched by the presence of 20β-dihydroprednisolone or any other prednisolone metabolite. It is also evident that this irregular endogenous prednisolone biosynthesis cannot be directly attributed to a specific organ (liver or adrenal glands) and further research is needed to ascertain the possible sites of endogenous prednisolone synthesis in naturally stressed cows.…”

Section: Discussionsupporting

confidence: 58%

Effects of truck transportation and slaughtering on the occurrence of prednisolone and its metabolites in cow urine, liver, and adrenal glands

et al. 2019

Self Cite

View full text Add to dashboard Cite

Background The recognition of illegal administration of synthetic corticosteroids in animal husbandry has been recently challenged by the case of prednisolone, whose occasional presence in the urine of bovines under strong stressful conditions was attributed to endogenous biosynthesis, not to exogenous administration. The study of the natural stress sources possibly inducing endogenous prednisolone production represents a stimulating investigation subject. The biochemical effects of transportation and slaughtering were verified in untreated cows by studying the possible occurrence of prednisolone and its metabolites in urine, liver and adrenal glands, and the cortisol/cortisone quantification. Results Cortisol, cortisone, prednisolone and its metabolites were measured in urine, collected at farm under natural micturition and then at the slaughterhouse. The study was performed on 15 untreated cows reared in different farms at the end of their productive cycle. 2–3 days after the first urine collection, the animals were transported by trucks to the abattoir, slaughtered, and subjected to a second urine sampling from the bladder. Specimens of liver and adrenal gland were also collected and analysed by means of a liquid chromatography-tandem mass spectrometry (LC-MS/MS) validated method. The stressful conditions of transportation and slaughtering proved to increase considerably the urinary levels of cortisol and cortisone as compared to those collected at farm. Prednisolone was detected in the urine collected at the slaughterhouse of two cows only, at a concentration level (≈0.6 μg L− 1) largely below the official cut off (5.0 μg L− 1) established to avoid false non-compliances. These two animals exhibited the highest urinary cortisol levels of the series. Prednisolone and prednisone were also detected in the adrenal glands of a different cow. Prednisolone metabolites were not detected in any urine, liver, and adrenal gland sample. Conclusion Within the constraints of the condition adopted, this study confirms the sporadic presence of prednisolone traces (2 samples out of 15) and the consistently increased concentration of cortisone and cortisol in the urines collected from cows subjected to truck transportation and subsequent slaughtering. No prednisolone metabolites were detected in any liver and adrenal gland samples, nor in urine specimens, unlike what was previously reported for cows artificially stressed by pharmacological treatment.

show abstract

Section: Discussionsupporting

confidence: 58%

Effects of truck transportation and slaughtering on the occurrence of prednisolone and its metabolites in cow urine, liver, and adrenal glands

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

Binding the gap between experiments, statistics, and method comparison: A tutorial for computing limits of detection and quantification in univariate calibration for complex samples

Gegenschatz

Chiappini

Teglia

et al. 2022

Analytica Chimica Acta

View full text Add to dashboard Cite

Corticosteroids in liver and urine in Sicilian cattle by a LC-MS/MS method

Giaccone

Macaluso

Cammilleri

et al. 2018

Food Additives & Contaminants: Part B

View full text Add to dashboard Cite

The presence of corticosteroid residues was assessed in urine and liver samples from livestock of Sicily. A total of 630 bovine samples were collected from farms and slaughterhouses. The samples were analysed using solid-phase extraction (SPE) coupled with ultra-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). All the corticosteroids found were under the maximum residue limit imposed by Commission Regulation (EC) 37/2010. About 4% of liver samples showed dexamethasone levels above the limit of detection (LOD), with a mean of 1.5 ± 0.2 µg kg. Betamethasone was found only in seven liver samples, with a mean of 1.6 ± 0.1 µg kg. Furthermore, prednisolone and prednisone were found only in urine and liver samples from slaughterhouse, probably related to the high rate of stress for bovines. These results suggest good control practices adopted by Sicilian farms, able to ensure the quality of food products.

show abstract

Determination of endogenous and exogenous corticosteroids in bovine urine and effect of fighting stress during the “Batailles des Reines” on their biosynthesis

Cited by 4 publications

References 19 publications

Effects of truck transportation and slaughtering on the occurrence of prednisolone and its metabolites in cow urine, liver, and adrenal glands

Effects of truck transportation and slaughtering on the occurrence of prednisolone and its metabolites in cow urine, liver, and adrenal glands

Binding the gap between experiments, statistics, and method comparison: A tutorial for computing limits of detection and quantification in univariate calibration for complex samples

Corticosteroids in liver and urine in Sicilian cattle by a LC-MS/MS method

Contact Info

Product

Resources

About