Determination of Dynamic Viscosity in Samples of Blood Plasma and Hemoglobin Solution Using Nuclear Magnetic Resonance

Abstract

The viscosity evaluation in Plasma is extremely useful in the clinical evaluation of different diseases. A procedure is presented, based on Protonic Magnetic Resonance, for the evaluation of the dynamic viscosity in Blood Plasma and in hemoglobin solution from the determination of the transverse relaxation time (T 2). To experimentally determine the T 2 value, the impulse series Carr-Purcell-Meiboon-Gill was used in a MARAN DRX console (OXFORD INSTRUMENTS) and a homogeneous magnetic system (B 0 = 0.095T). Values were obtained for the viscosity of the blood plasma and hemoglobin of 1.68 ± 0.12 mPas and 12.78 ± 3.55 mPas respectively, which agreed with the determined, in the same samples, using an Ostwald viscometer (1, 45 ± 0.06 mPas for the plasma and 12.82 ± 3.35 mPas for the dissolution of hemoglobin). The dynamic viscosity of the blood plasma was determined in 236 patients with Multiple Myeloma (2.19 ± 0.58 mPas), 142 with Drepanocytic Anemia (2.20 ± 0.79 mPas) showing statistically significant increases with respect to the characteristic values of the controls (1.68 ± 0.12mPas). Magnetic Relaxation is an option to evaluate plasma viscosity because it minimizes the volume of sample needed and eliminates the need to wash the viscometer between determinations. Magnetic Relaxation can compensate its relative high cost, compared with other Viscosimetry methods, facilitating other determinations of utility in several diseases.