Determination of drug protein-binding by high-performance liquid chromatography using a chemically bonded bovine albumin stationary phase

Measurements of drug–protein binding by using immobilized human serum albumin liquid chromatography–mass spectrometry

“…Therefore, using immobilized AAG column for quantitation of drug protein binding still remains questionable. (2) Compound structure diversity, which leads to binding mechanism complexity [24,26]. De Jong and coworkers [24] have shown that a better correlation was obtained with a series of structurally analogous piperazines compared to the structurally heterogeneous compounds.…”

“…(2) Compound structure diversity, which leads to binding mechanism complexity [24,26]. De Jong and coworkers [24] have shown that a better correlation was obtained with a series of structurally analogous piperazines compared to the structurally heterogeneous compounds. In order to elute compounds F and G, the mobile phase was modified with higher organic composition [30].…”

“…[21][22][23][24][25][26]32,34,5,18]. In this method, a small volume of solution containing the drug of interest is injected to immobilized HSA column, and the capacity factor (k ) is used to evaluate the protein binding strength of the drug.…”

“…It has been claimed by many research groups [1,21,24] that the extent of drug protein binding correlates better with the expression of k /(k + 1) than k . Therefore, drug-protein binding percent (%binding) obtained by conventional method is then regressed against k /(k +1), generating a quantitative working curve [21].…”

Determination of drug-plasma protein binding using human serum albumin chromatographic column and multiple linear regression model

“…For heterogeneous groups of compounds, in the past low correlation coefficients have been calculated. This demonstrates that the initial method applies only for structurally related compounds (Lammers et al, 1989).…”