Determination of doxycycline’s plasma protein binding rates in the plasma of grass carp ( Ctenopharyngodon idella ), yellow catfish ( Pelteobagrus fulvidraco ) and crayfish ( Procambarus clarkii ) by an ultrafiltration method at different temperatures with different concentrations

Abstract

The objective of this study was to compare the plasma protein binding rates (PPBRs) of doxycycline (DC) in the plasma of grass carp (Ctenopharyngodon idella), yellow catfish (Pelteobagrus fulvidraco) and crayfish (Procambarus clarkii) at different temperatures (4, 18 and 25℃) with different concentrations (1, 5, 10 and 20 μg/ml). A rapid and simple method of ultrafiltration (UF) was employed to determine the PPBR of DC in the plasma. The results showed that PPBRs were increased with the rise of the concentration of DC at the same temperature. At 4 ℃, PPBRs were increased from 90.00% to 99.51%, 89.81% to 98.71% and 84.08% to 99.21% in the plasma of grass carp, yellow catfish and crayfish respectively. At 18℃ and 25℃, the same trend was also presented in the three species. Meanwhile, PPBRs of DC might have the atypical nonlinear phenomenon. Otherwise, the temperature could affect the PPBR of DC at a certain degree in the plasma of grass carp and yellow catfish at the same concentration of DC. However, no regularity was presented in the plasma of crayfish. Overall, the increased concentration of DC significantly affected the PPBR in the plasma of grass carp, yellow catfish and crayfish at the same temperature, but the temperature only had a certain influence on the PPBR of grass carp and yellow catfish. Hence, this study is beneficial to design suitable therapeutic regimens and formulations of DC in grass carp, yellow catfish and crayfish at different temperatures.

Effects of Temperature on Plasma Protein Binding Ratios (PPBRs) of Enrofloxacin and Ciprofloxacin in Yellow Catfish (Pelteobagrus fulvidraco), Grass Carp (Ctenopharyngodon idella), and Largemouth Bass (Micropterus salmoides)

“…The PPBRs of EF at 15 • C were higher than that at 25 • C in the plasma of yellow catfish at 5 and 10 µg/mL except for 1 µg/mL. This finding is consistent with the report of doxycycline in grass carp and yellow catfish [35]. These suggested that, under higher temperatures, a smaller amount of EF was bound to plasma protein.…”

“…The PPBRs of tetracyclines increased all the time with the ascent of concentration caused by the atypical nonlinearity of the PPBR for tetracyclines. In previous studies, this phenomenon was also found in the PPBR of doxycycline, minocycline, and tigecycline [33][34][35]. The reason may be attributable to tetracyclines strongly chelating divalent cations to form complexes that are irreversible.…”

Phytoplankton Biological Pump Controls the Spatiotemporal Bioaccumulation and Trophic Transfer of Antibiotics in a Large Subtropical River

“…Significant positive correlations were observed between the fraction of plasma protein and ENX, CFX, NFX, and SPD concentrations. The binding rates of antibiotics to plasma proteins were positively correlated with the concentrations of antibiotics according to a recent study . To the best of our knowledge, the analytical measurement of the concentrations of antibiotics from biological samples for the first time shows an association with paired fractions of plasma proteins.…”

“…The binding rates of antibiotics to plasma proteins were positively correlated with the concentrations of antibiotics according to a recent study. 69 To the best of our knowledge, the analytical measurement of the concentrations of antibiotics from biological samples for the first time shows an association with paired fractions of plasma proteins. A limited number of studies have examined the differences in antibiotic uptake and accumulation among organisms occupied at different TLs.…”

Pharmacokinetic behaviour and pharmacokinetic–pharmacodynamic integration of doxycycline in rainbow trout (Oncorhynchus mykiss) after intravascular, intramuscular and oral administrations

“…It has been determined that DO has a variable volume of distribution (0.79-7.47 L/kg) in previous studies in fish, such as yellow catfish (Pelteobagrus fulvidraco), tilapia and grass crap (Holmes & Charles, 2009;Xu et al, 2020bXu et al, , 2021. In general, although DO is highly bound to plasma proteins in fish (grass crap 97.97% and yellow catfish 99.15%), it has a wide tissue distribution due to its lipophilic nature (Sun et al, 2022;Xu et al, 2019a). Additionally, DO revealed a limited volume of distribution in the vitreous with low protein concentrations (Gilmour et al, 2005).…”