Determination of doxorubicin hydrochloride by visible spectrophotometry

Sastry, C. S. P.; Rao, J. Babu

doi:10.1016/0039-9140(96)01932-7

“…The clinical value of these agents is limited by an unusual cardiomyopathy, the occurrence of which is related to the total dose of the drug, and is often irreversible. Only a few methods such as visible spectrophotometry [1], fluorimetry [2], room temperature phosphorescence [3], voltammetry [4], radiolabelled assays [5], thin-layer chromatography [6], and liquid chromatography [7±10] have been proposed for the determination of the anthracycline antibiotics.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of doxorubicin, daunorubicin, and idarubicin by capillary electrophoresis with laser-induced fluorescence detection

Pérez-Ruíz¹,

Martı́nez-Lozano²,

Sanz³

et al. 2001

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The separation and simultaneous determination of doxorubicin, daunorubicin and idarubicin was investigated using capillary electrophoresis with laser-induced fluorescence detection. Because the three anthracycline antibiotics were similar in structure and mass, careful manipulation of the electroosmotic flow and electrophoretic mobilities was required. A buffer consisting of 100 mM borate, adjusted to pH 9.5, containing 30% acetonitrile was found to provide a very efficient and stable electrophoretic system for the analysis of the three anthracyclines. The method was applied to the determination of three anthracyclines in serum samples. Responses were linear in the range of 10-500 ng.mL-1 and the detection limits were lower than 0.9 ng.mL-1.

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“…The clinical value of these agents is limited by an unusual cardiomyopathy, the occurrence of which is related to the total dose of the drug, and is often irreversible. Only a few methods such as visible spectrophotometry [1], fluorimetry [2], room temperature phosphorescence [3], voltammetry [4], radiolabelled assays [5], thin-layer chromatography [6], and liquid chromatography [7±10] have been proposed for the determination of the anthracycline antibiotics.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of doxorubicin, daunorubicin, and idarubicin by capillary electrophoresis with laser-induced fluorescence detection

Pérez-Ruíz

¹

,

Martı́nez-Lozano

²

,

Sanz

³

et al. 2001

View full text Add to dashboard Cite

The separation and simultaneous determination of doxorubicin, daunorubicin and idarubicin was investigated using capillary electrophoresis with laser-induced fluorescence detection. Because the three anthracycline antibiotics were similar in structure and mass, careful manipulation of the electroosmotic flow and electrophoretic mobilities was required. A buffer consisting of 100 mM borate, adjusted to pH 9.5, containing 30% acetonitrile was found to provide a very efficient and stable electrophoretic system for the analysis of the three anthracyclines. The method was applied to the determination of three anthracyclines in serum samples. Responses were linear in the range of 10-500 ng.mL-1 and the detection limits were lower than 0.9 ng.mL-1.

show abstract

“…The assay of doxorubicin·HCl in pure pharmaceutical formulations by periodate oxidative coupling of dialdehydes produced via Malaprade reaction with MBTH or with phenyl hydrazine·HCl in the presence of potassium ferrihexacyanide complex followed by spectrophotometric measurements at 620-670 nm or at 510 nm, respectively [84].…”

Section: Periodate Oxidative Coupling Reaction Methodsmentioning

confidence: 99%