Determination of donepezil in serum samples using molecularly imprinted polymer nanoparticles followed by high‐performance liquid chromatography with ultraviolet detection

Abstract

A molecularly imprinted polymer designed for the selective extraction of donepezil from serum samples was synthesized using a noncovalent molecular imprinting approach. The molecularly imprinted polymer was evaluated chromatographically and then its affinity for donepezil was confirmed by solid-phase extraction. The optimal conditions for solid-phase extraction were provided by cartridge conditioning using acidified water purified from a Milli-Q system, sample loading under basic aqueous conditions, clean-up using acetonitrile, and elution with methanol/tetrahydrofuran. Desirable molecular recognition properties of the molecularly imprinted polymer led to good donepezil recoveries (90-102%). The data indicated that the imprinted polymer has a perfect selectivity and affinity for donepezil and could be used for selective extraction and analysis of donepezil in human serum.

Synthesis of Surface Molecularly Imprinting Polymers for Methylphenidate and its Application in Separating Methylphenidate

“…The limit of quantification (LOQ) of assay (signal/noise ratio of 20) was 10 ng.mL -1 which was much lower than the minimum therapeutic concentration of MPH in patient serum. LOD and LOQ values obtained in other studies (with similar MISPE method and HPLC assay carried out for other templates) ranged from ng mL -1 to μg mL -1 [14,21]. Also, LOD and LOQ values of HPLC assay of MPH in human serum, determined by other researchers, were less than 1 ng mL -1 and 50 ng mL -1 , respectively [4].…”

“…Less polar solvents such as chloroform or hexane increase complex formation, facilitating polar non-covalent interactions such as hydrogen bonding. On the other hand, more polar solvents tend to dissociate the non-covalent interactions in the pre-polymer complex, especially protic solvents which leads to a high degree of disruption to hydrogen bonds [20][21][22]. In this work, two organic solvents (hexane and acetic acid) were tested as the polymerization solvents for optimization of molecular imprinting procedure (Figure 2).…”

“…This indicated a significantly higher affinity of MIP-A1 for methylphenidate in comparison with other drugs that could be present with methylphenidate, simultaneously, in serum of patients. We have also used ACN, in our previous studies, as washing solvent for selective extraction of donepezil in a MISPE method from their aqueous mixture [21]. Thus, optimized MISPE conditions were as follows: washing conditions, 3 mL acetic acid; elution conditions, 8 mL methanol-TFA (50:50, v/v).…”

Separation of microRNA 21 as a cancer marker from glioblastoma cell line using molecularly imprinted polymer coated on silica nanoparticles

“…Current methods of miRNA detection are mainly based on PCR , microarray , Northern blotting , and solid‐phase hybridization techniques . However, detection of miRNAs with high sensitivity still remains a great challenge .…”

Analysis of melamine in milk powder by using a magnetic molecularly imprinted polymer based on carbon nanotubes with ultra high performance liquid chromatography and tandem mass spectrometry

“…However, the adsorption capacity of the polymers is low and their application is not convenient . Due to low recovery rate and efficiency of ordinary MIP, magnetic molecularly imprinted polymer (MMIP) has received considerable attention and extensive application . The advantage of MMIP is separating from extraction system easily by the applied magnetic field .…”