Determination of DNA Methyltransferase 1 in Cells Using a RG108-Fluorescein Conjugate to Monitor the Fluorescent Ratio with a Microplate Reader

A review on recent advances in assays for DNMT1: a promising diagnostic biomarker for multiple human cancers

“…Another bioimaging probe for intracellular DNMT1 was synthesized based on the inhibitor RG108. 127,128 Fluorescein isothiocyanate (FITC) is a versatile agent used for cell imaging by confocal microscopy and widely used in labeling different biomolecules since the isothiocyanate group can couple with amino groups in protein and peptide chains. 129 As shown in path II in Fig.…”