Determination of Disodium Cromoglycate in Human Urine by High-performance Liquid Chromatography With Post-column Photoirradiation–Fluorescence Detection

Abstract

For the determination of disodium cromoglycate in urine, a fluorimetric method using HPLC post-column photoirradiation has been developed. The mobile phase consisted of a 35 mmol l-1 phosphate buffer (pH 8)-methanol (7 + 3, %v/v) containing 75 mmol l-1 hydrogen peroxide and 20 mmol l-1 18-crown-6. The 18-crown-6 was used for separation adjustment of the disodium cromoglycate in the urine sample. Photoirradiation was carried out in tubing wound around a germicidal light in a reactor equipped with an air-cooling fan. The fluorescence was monitored with excitation at 325 nm and emission at 448 nm. The calibration graph for disodium cromoglycate was linear over the range 38-2340 ng ml-1 using an injection volume of 100 microliters. The pretreatment of the urine samples consisted of diluting and filtering steps. The mean recovery of disodium cromoglycate from urine was 99.1 +/- 2.4% (n = 6).

Tobramycin as a Pharmacologic Tracer to Compare Airway Deposition from Nebulizers

“…Quantification of cromolyn sodium, nedocromil, and albuterol in urine can be done but must be performed with high-performance liquid chromatographic techniques that are relatively complex and expensive. [36][37][38] Using urinary excretion of tobramycin as a bioassay to document pulmonary delivery is not without limitations. The most obvious is that it does not describe the location in the airways where absorption of tracer occurs.…”

Simultaneous Determination of Kynurenine and Kynurenic Acid by High-Performance Liquid Chromatography Photoirradiation System Using a Mobile Phase Containing 18-crown-6

“…It have been reported that the retention time usually becomes longer by the addition of CE in mobile phase. 11,12 The mechanism of reduction on the retention time is still not clear; however, it is speculated that the ion of phosphate buffer affects the retention time of KYNA. For separate of KYN and KYNA from components in the serum, the pH of phosphate buffer was adopted to 8.0, which gave retention times for KYN and KYNA of 18 and 24 minutes, respectively.…”

Spectral resolution and simultaneous determination of oxymetazoline hydrochloride and sodium cromoglycate by derivative and ratio-based spectrophotometric methods

“…Both drugs are co-formulated in a nasal spray dosage form and are widely used for effective treatment of nasal congestion and allergy. Several methods have been reported for the determination of SCG in pharmaceutical preparations such as spectrophotometry [3,4], electrophoresis [5], electrochemical [6,7] and HPLC methods [8][9][10][11][12][13][14]. OXMT has also been analyzed by several methods in pharmaceutical preparations such as spectrophotometry [3,[15][16][17][18][19][20][21][22][23], capillary electrophoresis [24,25], gas chromatography [26,27] and HPLC [28][29][30][31][32][33][34][35].…”