Determination of Dipicolinic Acid in “Natto” by High-Performance Liquid Chromatography Coupled With Postcolumn Photoirradiation With Zinc Acetate

Abstract

A system was developed for determining dipicolinic acid in “natto” using liquid chromatography with fluorometric detection. The compound was separated by reversed-phase chromatography using a mobile phase of 0.1 mol/L disodium hydrogen phosphate, 0.05 mol/L citric acid buffer (adjusted to pH 3.0) containing 3.0 mmol/L zinc acetate and 35 mmol/L perchloric acid. The compound in the column effluent was irradiated with ultraviolet light to produce fluorescence. This fluorescence was monitored at an excitation at 336 nm and an emission at 448 nm. The calibration curve for dipicolinic acid was observed to be linear in a range of 0.2 to 112 ng. The dipicolinic acid content of natto was 7.24 ± 0.54 mg/100 g (wet weight, mean ± standard deviation [SD], n = 6).

Eu3+-MOF fluorescence sensor based on a dual-ligand strategy for visualised detection of an anthrax biomarker 2,6-pyridine dicarboxylic acid

“…[6][7][8] Therefore, accurate measurement of DPA is of the utmost importance to prevent anthrax infection and protect public health. 9 At present, many methods have been investigated for detecting DPA, such as high-performance liquid chromato-graphy, 10 polymerase chain reaction, 11 surface enhanced Raman spectroscopy, 12 immunoassays, 13 and fluorescence spectroscopy. 14,15 Fluorescent sensors have demonstrated application potentials because of their simple operation, high sensitivity, portability, high efficiency, and low cost.…”

A Benzothiadiazole-Based Zn(II) Metal–Organic Framework with Visual Turn-On Sensing for Anthrax Biomarker and Theoretical Calculation

“…Consequently, accurately identifying the DPA level is essential for preventing disease outbreaks and safeguarding homeland security. DPA quantification has been evaluated by Raman spectroscopy [ 8 ], polymerase chain reaction (PCR) [ 9 ], electrochemical methods [ 10 ], liquid chromatography [ 11 ], etc. However, the majority of the above methods are typically constrained by the inefficiencies of time-consumption pretreatment and high equipment costs.…”

Determination of Picolinic Acid by HPLC Coupled With Postcolumn Photo Irradiation Using Zinc Acetate as a Fluorescent Derivatization Reagent

“…Apply this method, developed a fluorometric system for determination of dipicolinic acid in fermented soybeans. 15 That method, phosphate-citric acid buffer solution, in which zinc acetate hardly precipitates, was used as the mobile phase, but it was difficult to separate the peaks of serum contaminants and picolinic acid in this buffer solution. Therefore, a measurement method of picolinic acid in serum was established by using sodium phosphate solution which is easy to precipitate zinc acetate but is easy to separate from contaminants, and by combining a pretreatment method with good washability and recovery.…”