Determination of diminazene aceturate in pharmaceutical formulations by HPLC and identification of related substances by LC/MS

Atsriku, Christian; Watson, David; Tettey, J.N.A.; Grant, M.H.; Skellern, G.G.

doi:10.1016/s0731-7085(02)00450-8

Cited by 37 publications

(34 citation statements)

References 3 publications

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“…This is indicative of p-aminobenzamidine, a decomposition product of diminazene. These data are consistent with the LC-MS analysis of pharmaceutical preparation of diminazene [11]. However, for the purposes of this study, additional diagnostic ions were required in order to meet established confirmation criteria [13].…”

Section: Ion Trap Ms Analysis Of Diminazenesupporting

confidence: 73%

“…An LC-MS method developed to characterize pharmaceutical preparations of diminazene utilized a reversed phase LC column with an ammonium formate:acetonitrile mobile phase [11]. These chromatographic conditions were used as a starting point to develop and validate the LC-MS n method reported here.…”

Section: Optimization Of Chromatographic Parametersmentioning

confidence: 99%

“…For a complete analytical data package, it is also important to obtain complementary MS data to confirm the residue's presence in food animals at these levels. While there is one report that characterizes diminazene and its impurities in pharmaceutical formulations by LC-MS [11], a MS confirmatory procedure for residue levels (ng/g) of this drug in animal fluids has not been published. This paper describes an LC-MS method for the positive identification of diminazene in bovine plasma.…”

Section: Introductionmentioning

confidence: 99%

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Confirmation of diminazene diaceturate in bovine plasma using electrospray liquid chromatography–mass spectrometry

Turnipseed

Clark

Andersen

et al. 2006

Journal of Chromatography B

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Section: Ion Trap Ms Analysis Of Diminazenesupporting

confidence: 73%

Section: Optimization Of Chromatographic Parametersmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Confirmation of diminazene diaceturate in bovine plasma using electrospray liquid chromatography–mass spectrometry

Turnipseed

Clark

Andersen

et al. 2006

Journal of Chromatography B

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“…The power of LC combined with an universal detector is an optimal combination to determine related substances and peak homogeneity. Selected articles are referenced for the reader [30,49,50].…”

Section: Implementation Of Alternative Technologiesmentioning

confidence: 99%

Role of HPLC During Formulation Development

Patel

LoBrutto

2006

HPLC for Pharmaceutical Scientists

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“…1 It is an official drug in USP 2 and BP 3 pharmacopeia. Several analytical methods were reported for the determination of AN either alone or coformulated with other drugs including spectrophotometric, 4,5 chemometric, 6,7 HPLC, 8,9 TLC, 10,11 GC,12,13 , capillary zone electrophoresis 14 and non aqueous titration 15 methods. Benzocaine (BE, Fig.…”

Section: Introductionmentioning

confidence: 99%

Simple spectrophotometric methods for the simultaneous determination of antipyrine and benzocaine

Merey

2016

Bulletin of Faculty of Pharmacy, Cairo University

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Antipyrine and benzocaine are formulated together for the treatment of ear inflammation and to relieve pain. Four spectrophotometric methods were developed for the simultaneous determination of antipyrine (AN) and benzocaine (BE) in their combined dosage form. Method A depends on applying dual wavelength method where antipyrine was determined by measuring the absorbance at 254.1 and 309.1 nm (corresponding to zero difference of benzocaine), while the absorbance difference at 230.1 and 263.5 nm (corresponding to zero difference of antipyrine) was selected for benzocaine determination in the laboratory prepared spectrum. Method B depends on measuring the peak amplitude of first derivative at 305 nm for calculating benzocaine concentration then the total concentration of both drugs was determined using isoabsorptive point at 257.4 nm (antipyrine concentration was then calculated by subtraction). Method C is based on measuring the peak difference of the ratio spectra at Dp (239.1-285 nm) and Dp (301.4-250 nm) for the determination of antipyrine and benzocaine, respectively. Method D depends on measuring peak to peak amplitude of the first derivative of ratio spectra at (234.5 + 244.2 nm) and peak amplitude at 295.5 nm for the determination of antipyrine and benzocaine, respectively. The proposed methods were validated and applied for the analysis of antipyrine and benzocaine in their laboratory prepared mixtures and pharmaceutical formulation. Statistical comparison between the results of the proposed methods and those of the reported methods showed no significant difference. Ó 2016 Production and hosting by Elsevier B.V. on behalf of Faculty of Pharmacy, Cairo University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/ 4.0/).

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Determination of diminazene aceturate in pharmaceutical formulations by HPLC and identification of related substances by LC/MS

Cited by 37 publications

References 3 publications

Confirmation of diminazene diaceturate in bovine plasma using electrospray liquid chromatography–mass spectrometry

Confirmation of diminazene diaceturate in bovine plasma using electrospray liquid chromatography–mass spectrometry

Role of HPLC During Formulation Development

Simple spectrophotometric methods for the simultaneous determination of antipyrine and benzocaine

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