Determination of dimethylamine and other low-molecular-mass amines using capillary electrophoresis with laser-induced fluorescence detection

Da̧bek-Złotorzyńska, Ewa; Maruszak, Wioleta

doi:10.1016/s0378-4347(98)00070-x

Cited by 59 publications

(41 citation statements)

References 48 publications

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“…Compared with other amino-labeled fluorescence reagent, such as FITC and TRITC, 13,14 Cy5 showed the higher reaction selectivity and shorter reaction time for primary amines. And the detection limits of Cy5-derivatized amines were the same as those of FITC-derivatized amines.…”

Section: Resultsmentioning

confidence: 98%

“…And the detection limits of Cy5-derivatized amines were the same as those of FITC-derivatized amines. 13 …”

Section: Resultsmentioning

confidence: 99%

“…13,14 The result showed the derivatization efficiencies for noradrenaline and dopamine increasing with time; after 90 min, a plateau was reached. Then the derivatization procedure tended to stabilize.…”

Section: Effect Of Reaction Time On Derivatization Reactionmentioning

confidence: 97%

“…10 The detection methods of amine in CE include UV-VIS, 11 electrochemical detection 12 and LIF. 13 LIF is one of the most sensitive detection techniques in CE because of its low background signal and the small sample volume. In this paper, we report a rapid, sensitive method for the analysis of noradrenaline and dopamine in CE coupled diode LIF detection, in which Cy5 was chosen as the fluorescence derivative reagent.…”

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confidence: 99%

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Fluorescent Determination of Noradrenaline and Dopamine Derivatized with Cy5 in Capillary Electrophoresis

Zhang

et al. 2001

ANAL. SCI.

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Noradrenaline and dopamine are two of the most important monoamine neurotransmitters. They play important roles in learning and memory, and in regulation of cardiovascular activity and nervous system. Analysis of the monoamine neurotransmitters has conventionally used high performance liquid chromatography (HPLC) 1-3 or gas chromatography. 4,5 However, the determination of monoamine neurotransmitters in the biological matrix is very difficult because the compounds are usually present at very low concentrations, and many other compounds in biological matrix, which have also absorption in the low-wavelength region, interfere badly with the analysis of monoamine neurotransmitters. Cyanine derivative (Cy5) is a new fluorescence derivative reagent. The excitation and the emission wavelength of Cy5 are 649 and 670 nm, respectively. Thus diode lasers, which emit wavelengths from deep-red to infrared region, are chosen as the light source for laser-induced fluorescence (LIF). This is preferential for determining Cy5-labeled amine neurotransmitters, since almost all of the biological substances have no absorption band in the deep-red and infrared region, resulting in a remarkable decrease of background fluorescence noise and in an increase of detection sensitivity. Recently, LIF is widely used in the analysis of DNA sequence, oligonucleotides and proteins. 6-8But determination of Cy5-labeled monoamine neurotransmitters has not been reported. Capillary electrophoresis (CE) is a newly emerging technique for rapid and high-resolution separation of biomolecules.9 Recently, CE is widely used in the separation and determination of amine. 10 The detection methods of amine in CE include UV-VIS, 11 electrochemical detection 12 and LIF. 13 LIF is one of the most sensitive detection techniques in CE because of its low background signal and the small sample volume. In this paper, we report a rapid, sensitive method for the analysis of noradrenaline and dopamine in CE coupled diode LIF detection, in which Cy5 was chosen as the fluorescence derivative reagent.The effects of buffer concentration, pH value, and reaction time on the derivatization reaction of monoamines were investigated. Experimental ReagentsUnless stated otherwise, all chemicals were of analyticalreagent grade (Beijing Reagent Corp., Beijing, China). Noradrenaline, dopamine and sodium dodecyl sulfate (SDS) were purchased from Sigma (St. Louis, MO, USA). Cy5 was obtained from Amersham Pharmcia Biotech, USA. Water was deionized and purified by a Milli-Q Millipore purification system (Millipore, Marlborough, MA, USA). ApparatusAll analyses were performed on a Beckman P/ACE 5000 system equipped with a Beckman Laser Module 635 nm. This was used as an exciting light source (Beckman-Fullerton, CA, USA). Separations were performed in fused-silica capillaries (57 cm × 75 µm i.d.; Yong Nian Optic Fiber Factory, Hebei Province, China). Derivatization procedureNoradrenaline and dopamine were labeled with Cy5 as follows: Cy5 was dissolved in dimethylformamide. The solution with a...

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Section: Resultsmentioning

confidence: 98%

“…And the detection limits of Cy5-derivatized amines were the same as those of FITC-derivatized amines. 13 …”

Section: Resultsmentioning

confidence: 99%

Section: Effect Of Reaction Time On Derivatization Reactionmentioning

confidence: 97%

mentioning

confidence: 99%

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Fluorescent Determination of Noradrenaline and Dopamine Derivatized with Cy5 in Capillary Electrophoresis

Zhang

et al. 2001

ANAL. SCI.

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“…Less common separation methods such as supercritical fluid chromatography (SFC) (22)(23)(24) and capillary electrophoresis (CE) (25) have also been used for the analysis of small amines. Good results could be obtained for alkaline extracts of atmospheric aerosol samples, especially for the combination of CE with laser induced fluorescence detection that enabled very low detection limits (~1 ng/L).…”

Section: Introductionmentioning

confidence: 99%

Determination of Amines in the Presence of Excess Ammonia by Ion Chromatography-Mass Spectrometry

Hermans

Jonkers

Bokx

2010

Journal of Chromatographic Science

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A new method for the simultaneous determination of several amines in the presence of an excess of ammonia by ion chromatography-mass spectrometry detection was developed. Current methods using ion chromatography with suppressed conductivity detection are not selective enough to determine small amines at the required level of 10 µg/L or preferably lower in the presence of a large excess of ammonia (~1 mg/L) without resorting to time-consuming sample pre-treatment techniques. By using mass spectrometric detection, which is capable of resolving eluting compounds on their m/z values, an additional dimension of confirmation is added to the analysis. Detection based on the analytes m/z value overcomes problems such as co-elution or background interferences that complicate the quantification when using conductivity detection. The optimal conditions for mass spectrometry detection of amines in the presence of an excess of ammonia were investigated by a four-factor central composite design. The four factors investigated were scan time, cone voltage, probe temperature, and needle voltage. Evaluation of the obtained experimental data showed that detection limits were up to a factor of 100 lower when using mass spectrometry as the detection technique instead of the conventional suppressed conductivity detection. Detection limits of 1 µg/L and lower can be achieved for the six amines investigated in the presence of a large excess of ammonia (~1 mg/L).

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Capillary electrophoresis and capillary electrochromatography of organic pollutants

1999

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Determination of dimethylamine and other low-molecular-mass amines using capillary electrophoresis with laser-induced fluorescence detection

Cited by 59 publications

References 48 publications

Fluorescent Determination of Noradrenaline and Dopamine Derivatized with Cy5 in Capillary Electrophoresis

Fluorescent Determination of Noradrenaline and Dopamine Derivatized with Cy5 in Capillary Electrophoresis

Determination of Amines in the Presence of Excess Ammonia by Ion Chromatography-Mass Spectrometry

Capillary electrophoresis and capillary electrochromatography of organic pollutants

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