Determination of diffusion coefficients and molecular weights of partially dissociated F<sub>1</sub>‐ATPase from yeast by gel filtration coupled to laser light‐scattering spectroscopy

Doster, Wolfgang; Begusch, Helmut; Hess, Benno

doi:10.1016/0014-5793(80)80617-x

Cited by 3 publications

(1 citation statement)

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“…The predictions of diffusion coefficients based on the molecular weights were made according to the Young–Carroad–Bell model in aqueous solution at 20 °C (ref ). The diffusion coefficients of enzymes from FCS measurements (solid circles) can be found in Table ; the reported diffusion coefficients from other methods (open circles) are hexokinase, 56.4 μm 2 /s, ref ; aldolase, 46.3 μm 2 /s, ref ; catalase, 41 μm 2 /s, ref ; F 1 -ATPase, 31 μm 2 /s, ref ; and urease, 31 μm 2 /s, ref .…”

Section: Experimental Observationsmentioning

confidence: 99%

Enhanced Diffusion of Catalytically Active Enzymes

Zhang

Hess

2019

ACS Cent. Sci.

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The past decade has seen an increasing number of investigations into enhanced diffusion of catalytically active enzymes. These studies suggested that enzymes are actively propelled as they catalyze reactions or bind with ligands (e.g., substrates or inhibitors). In this Outlook, we chronologically summarize and discuss the experimental observations and theoretical interpretations and emphasize the potential contradictions in these efforts. We point out that the existing multimeric forms of enzymes or isozymes may cause artifacts in measurements and that the conformational changes upon substrate binding are usually not sufficient to give rise to a diffusion enhancement greater than 30%. Therefore, more rigorous experiments and a more comprehensive theory are urgently needed to quantitatively validate and describe the enhanced enzyme diffusion.

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Section: Experimental Observationsmentioning

confidence: 99%

Enhanced Diffusion of Catalytically Active Enzymes

Zhang

Hess

2019

ACS Cent. Sci.

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The oligomycin sensitivity conferring protein (OSCP) of beef heart mitochondria: Studies of its binding to F1 and its function

Hundal

Norling

Ernster

1984

J Bioenerg Biomembr

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The binding of "oligomycin sensitivity conferring protein" (OSCP) to soluble beef-heart mitochondrial ATPase (F1) has been investigated. OSCP forms a stable complex with F1, and the F1 X OSCP complex is capable of restoring oligomycin- and DCCD-sensitive ATPase activity to F1- and OSCP-depleted submitochondrial particles. The F1 X OSCP complex retains 50% of its ATPase activity upon cold exposure while free F1 is inactivated by 90% or more. Both free F1 and the F1 X OSCP complex release upon cold exposure a part--probably 1 out of 3--of their beta subunits; whether alpha subunits are also lost is uncertain. The cold-treated F1 X OSCP complex is still capable of restoring oligomycin- and DCCD-sensitive ATPase activity to F1- and OSCP-depleted particles. OSCP also protects F1 against modification of its alpha subunit by mild trypsin treatment. This finding together with the earlier demonstration that trypsin-modified F1 cannot bind OSCP indicates that OSCP binds to the alpha subunit of F1 and that F1 contains three binding sites for OSCP. The results are discussed in relation to the possible role of OSCP in the interaction of F1 with the membrane sector of the mitochondrial ATPase system.

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The sulphydryl content of yeast mitochondrial F₁‐ATPase and the stoichiometry of subunits

Gregory

Hess

1981

FEBS Letters

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Determination of diffusion coefficients and molecular weights of partially dissociated F₁‐ATPase from yeast by gel filtration coupled to laser light‐scattering spectroscopy

Cited by 3 publications

References 7 publications

Enhanced Diffusion of Catalytically Active Enzymes

Enhanced Diffusion of Catalytically Active Enzymes

The oligomycin sensitivity conferring protein (OSCP) of beef heart mitochondria: Studies of its binding to F1 and its function

The sulphydryl content of yeast mitochondrial F₁‐ATPase and the stoichiometry of subunits

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Determination of diffusion coefficients and molecular weights of partially dissociated F1‐ATPase from yeast by gel filtration coupled to laser light‐scattering spectroscopy

Cited by 3 publications

References 7 publications

Enhanced Diffusion of Catalytically Active Enzymes

Enhanced Diffusion of Catalytically Active Enzymes

The oligomycin sensitivity conferring protein (OSCP) of beef heart mitochondria: Studies of its binding to F1 and its function

The sulphydryl content of yeast mitochondrial F1‐ATPase and the stoichiometry of subunits

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Product

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Determination of diffusion coefficients and molecular weights of partially dissociated F₁‐ATPase from yeast by gel filtration coupled to laser light‐scattering spectroscopy

The sulphydryl content of yeast mitochondrial F₁‐ATPase and the stoichiometry of subunits