Determination of diesel genotoxicity in firebreathers by micronuclei and nuclear abnormalities in buccal mucosa

Torres-Bugarı́n, Olivia; Anda-Casillas, A De; Ramı́rez-Muñoz, M.P; Sánchez‐Corona, José; Cantú, J. M.; G, Zúñiga

doi:10.1016/s1383-5718(98)00021-7

Cited by 57 publications

(26 citation statements)

References 24 publications

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“…Similarly, smoking increases the frequency of BN cells in both the exposed and control groups. Several studies demonstrate that occupational or habitual exposure to chemical agents, such as benzene, pesticides, alcohol, and cigarette smoke, increases the frequency of BN cells and causes cytotoxicity in the buccal epithelium [Torres-Bugarin et al, 1998;Gómez-Arroyo et al, 2000;Ramirez and Saldanha, 2002;Celik et al, 2003]. …”

Section: Discussionmentioning

confidence: 99%

Genotoxicity of occupational exposure to wood dust: Micronucleus frequency and nuclear changes in exfoliated buccal mucosa cells

Çelık

Kanık

2006

Environ and Mol Mutagen

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Occupational exposure to wood dust is associated with the occurrence of nasal cancer. In this study, we investigated micronuclei and nuclear changes (NCs: binucleates, karyorrhexis, karyolysis, and the "broken egg" effect) in exfoliated buccal cells of 20 workers exposed to wood dust and 20 age- and sex-matched controls. Micronucleus frequency and the frequency of each of the NCs were significantly higher for wood workers than controls (P < 0.01). Cigarette smoking was associated with increased frequencies of micronuclei and NCs in the buccal mucosa epithelium cells of both the control and exposed groups. Our findings indicate that buccal cells of wood workers display increased levels of genotoxicity and toxicity, and that these biomarker responses may be related to the increased cancer risk among wood workers.

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Section: Discussionmentioning

confidence: 99%

Genotoxicity of occupational exposure to wood dust: Micronucleus frequency and nuclear changes in exfoliated buccal mucosa cells

Çelık

Kanık

2006

Environ and Mol Mutagen

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“…A highly proliferating tissue like blood is required to carry out this assay [Lähdetie, 1983;Shuilin and Baker, 1989;Schmezer et al, 1990;Torres-Bugarín et al, 1998;Zamora-Perez et al, 2004], and only a drop of blood is needed to measure micronucleus (MN) frequencies in peripheral blood, so that it is not necessary to kill the animal [Zúñiga-González et al, 1998;Zúñiga-González et al, 2001b]. In some species, spontaneous MNEs can be observed in peripheral blood [Zúñiga et al, 1996a;Zúñiga et al, 2000;Zúñiga-González et al, 2001a], because the reticuloendothelial system inefficiently removes them from the circulation.…”

Section: Introductionmentioning

confidence: 99%

Micronucleated erythrocyte frequencies in old and new world primates: Measurement of micronucleated erythrocyte frequencies in peripheral blood ofCallithrix jacchus as a model for evaluating genotoxicity in primates

Zúñiga‐González

Gómez‐Meda

Zamora‐Pérez

et al. 2005

Environ. Mol. Mutagen.

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Nonhuman primates are of particular relevance in evaluating the potential toxicity of drugs and environmental agents. We have used previously published information and data from the present study to establish a relationship for New World (NW) and Old World (OW) primates on the basis of the frequency of spontaneous micronucleated erythrocytes (MNEs) observed in peripheral blood. Data on spontaneous MNEs in peripheral blood from 15 species of primates, including humans, indicate that NW primates have significantly (P < 0.01) higher MNE frequencies (group mean, 9.5 +/- 7.3 MNEs/10,000 erythrocytes; range, 0.7-20.5/10,000 erythrocytes) than OW primates (group mean, 1.0 +/- 0.9 MNEs/10,000 erythrocytes; range, 0.0-2.6 MNEs/10,000 erythrocytes). Humans are believed to have developed in the OW, and human MNE frequencies were similar to those described for OW primate species. We selected the common marmoset (Callithrix jacchus), a NW primate, to determine whether therapeutic pediatric doses of Metotrexate (MTX; 2.5 mg/kg), Cyclophosphamide (CP; 5 mg/kg), Cytosine-arabinoside (Ara-C; 3 mg/kg), or 5-Fluorouracil (5-FU; 10 mg/kg), administered daily for two consecutive days, increase the frequency of micronuclei. Micronucleated polychromatic erythrocyte frequencies were increased significantly in groups receiving MTX, CP and Ara-C, while MNE frequencies were increased by the Ara-C treatment. The results of this study indicate that NW primates have higher spontaneous MNE frequencies than OW primates, and because of this, NW primates like the common marmoset, may be suitable for evaluating the genotoxicity of chemical agents.

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“…This could be because MN formation is not a phenomenon exclusively related to exposure to tobacco, it could also reflect the effect of multitude of genotoxic agents like environmental pollutants, radiations or chemicals [12][13][14][15].…”

Section: Resultsmentioning

confidence: 99%

Comparative Study of Genotoxicity in Different Tobacco Related Habits using Micronucleus Assay in Exfoliated Buccal Epithelial Cells

Guruprasad

Jose

Saxena

et al. 2014

JCDR

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Background: Oral cancer is one of the most debilitating diseases afflicting mankind. Consumption of tobacco in various forms constitutes one of the most important etiological factors in initiation of oral cancer. When the focus of today's research is to determine early genotoxic changes in human cells, micronucleus (MN) assay provides a simple, yet reliable indicator of genotoxic damage. Aims and Objectives:To identify and quantify micronuclei in the exfoliated cells of oral mucosa in individuals with different tobacco related habits and control group, to compare the genotoxicity of different tobacco related habits between each group and also with that of control group. Patients and Methods:In the present study buccal smears of 135 individuals with different tobacco related habits & buccal smears of 45 age and sex matched controls were obtained, stained using Giemsa stain and then observed under 100X magnification in order to identify and quantify micronuclei in the exfoliated cells of oral mucosa. Results:The mean Micronucleus (MN) count in individuals having smoking habit were 3.11 while the count was 0.50, 2.13, and 1.67 in normal control, smoking with beetle quid and smokeless tobacco habit respectively. MN count in smokers group was 2.6 times more compared to normal controls. MN count was more even in other groups when compared to normal control but to a lesser extent. Conclusion:From our study we concluded that tobacco in any form is genotoxic especially smokers are of higher risk and micronucleus assay can be used as a simple yet reliable marker for genotoxic evaluation.

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Determination of diesel genotoxicity in firebreathers by micronuclei and nuclear abnormalities in buccal mucosa

Cited by 57 publications

References 24 publications

Genotoxicity of occupational exposure to wood dust: Micronucleus frequency and nuclear changes in exfoliated buccal mucosa cells

Genotoxicity of occupational exposure to wood dust: Micronucleus frequency and nuclear changes in exfoliated buccal mucosa cells

Micronucleated erythrocyte frequencies in old and new world primates: Measurement of micronucleated erythrocyte frequencies in peripheral blood ofCallithrix jacchus as a model for evaluating genotoxicity in primates

Comparative Study of Genotoxicity in Different Tobacco Related Habits using Micronucleus Assay in Exfoliated Buccal Epithelial Cells

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