Determination of dichloromethane, trichloroethylene and perchloroethylene in urine samples by headspace solid phase microextraction gas chromatography–mass spectrometry

Poli, Diana; Manini, Paola; Andreoli, Roberta; Franchini, I; Mutti, Antonio

doi:10.1016/j.jchromb.2005.03.009

Cited by 50 publications

(32 citation statements)

References 24 publications

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“…For the liquid-phase bioluminescence assays, the SPME fibers were inserted for 15 min into the culture flask headspace (n = 3). DCM measurements were taken hourly with the SPME [23]. SPME fibers were cleaned daily by insertion in the 280°C GC injection port for 5 min.…”

Section: Analytical Measurements Of Dcm Concentrationsmentioning

confidence: 99%

Detection of dichloromethane with a bioluminescent (lux) bacterial bioreporter

Lopes

Hawkins

Jegier

et al. 2012

Journal of Industrial Microbiology and Biotechnology

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The focus of this research effort was to develop an autonomous, inducible, lux-based bioluminescent bioreporter for the real-time detection of dichloromethane. Dichloromethane (DCM), also known as methylene chloride, is a volatile organic compound and one of the most commonly used halogenated solvents in the U.S., with applications ranging from grease and paint stripping to aerosol propellants and pharmaceutical tablet coatings. Predictably, it is released into the environment where it contaminates air and water resources. Due to its classification as a probable human carcinogen, hepatic toxin, and central nervous system effector, DCM must be carefully monitored and controlled. Methods for DCM detection usually rely on analytical techniques such as solid-phase microextraction (SPME) and capillary gas chromatography or photoacoustic environmental monitors, all of which require trained personnel and/or expensive equipment. To complement conventional monitoring practices, we have created a bioreporter for the self-directed detection of DCM by taking advantage of the evolutionary adaptation of bacteria to recognize and metabolize chemical agents. This bioreporter, Methylobacterium extorquens DCM( lux ), was engineered to contain a bioluminescent luxCDABE gene cassette derived from Photorhabdus luminescens fused downstream to the dcm dehalogenase operon, which causes the organism to generate visible light when exposed to DCM. We have demonstrated detection limits down to 1.0 ppm under vapor phase exposures and 0.1 ppm under liquid phase exposures with response times of 2.3 and 1.3 h, respectively, and with specificity towards DCM under relevant industrial environmental monitoring conditions.

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Section: Analytical Measurements Of Dcm Concentrationsmentioning

confidence: 99%

Detection of dichloromethane with a bioluminescent (lux) bacterial bioreporter

Lopes

Hawkins

Jegier

et al. 2012

Journal of Industrial Microbiology and Biotechnology

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“…establish biological exposure indexes in urine for metals and non-volatile organic compounds expressing them in mg/g creatinine. However, levels of volatile organic compounds in urine (LMMAs in this work) used as biomarkers of exposure must be expressed in concentrations, according to the literature [36][37][38] and the cited organisations. This is supported by the fact that at constant environmental concentrations, during exposure, equilibrium tends to be reached between partial pressure of volatile organic compounds in external air, alveolar air, arterial blood, body tissues and mixed venous blood.…”

Section: Analysis Of Urine Samplesmentioning

confidence: 99%

A novel clean-up method for urine analysis of low-molecular mass aldehydes by capillary electrophoresis with laser-induced fluorescence detection

Baños

Silva

2011

Journal of Chromatography B

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“…The abundance signal increased for the nine HAAs as the amounts of salt increased. A concentration of 6 g of Na 2 SO 4 per 12 ml of urine (saturated solution) was selected as the working value in order to homogenize urine samples since they contain different values of ionic strength [22]. The effect of the concentration of the derivatising reagent was studied using amounts of pure dimethylsulphate (DMS) between 50 and 200 l. The reaction yield increased as the volume rose to 110 l, above which it remained constant, but above 130 l the excess of DMS was extracted in n-pentane and volatilised, appearing in the chromatogram.…”

Section: Optimization Of the Chemical Variablesmentioning

confidence: 99%