Determination of desferrioxamine-available iron in biological tissues by high-pressure liquid chromatography

Gower, Jon D.; Healing, Guy; Green, Colin J.

doi:10.1016/0003-2697(89)90099-7

Cited by 40 publications

(33 citation statements)

References 19 publications

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“…Recalculation of the above results would imply that in mongrel dog brain and heart 10% ofthe total iron is in the low molecular weight iron pool under normoxic conditions. This is very high with regard to other estimates in which the low molecular weight iron pools amounts to only 0.2% (28) (20,21 ), additional iron release may have occurred during those incubations.…”

Section: Discussionmentioning

confidence: 59%

“…Contaminating iron from the chemicals used, determined by extrapolation of this curve to ferrioxamine/deferoxamine = 0, was consistently low at 2.1±0.2 nmol/ml (n = 3 separate curves). Both slope and background were similar to those presented by Gower et al (28). To measure the low molecular weight iron pool the 10,000 g supernatant was incubated with deferoxamine, aliquots are drawn at different time points, and the ferrioxamine/deferoxamine ratio was determined.…”

Section: Experimental Designmentioning

confidence: 66%

“…The chelated iron in normoxic rat hearts as measured in 60-min incubations by Gower et al (28) was 15.44±6.37 nmol/g wet weight. In our hands the 60-min incubations yielded 21.6±3.6 (n = 6) nmol chelated iron/g wet weight.…”

Section: Discussionmentioning

confidence: 89%

“…The very high stability ofthe complex ensures that all iron is extracted from the weaker intracellular chelators. In this study iron was measured as the ratio of ferrioxamine to deferoxamine formed in incubations at 37°C of 10,000 g supernatant ofheart homogenates with 2 mM deferoxamine as described by Gower et al (28). In this procedure all the iron in the low molecular weight pool is chelated within 1 h. However, it also leads to the removal ofiron from ferritin, which is linear in time over 2 2 h (29).…”

Section: Low Molecular Weight Iron Measurementsmentioning

confidence: 99%

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Low molecular weight iron and the oxygen paradox in isolated rat hearts.

Voogd¹,

Sluiter²,

Eijk³

et al. 1992

J. Clin. Invest.

111

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Little is known about changes in the amount of iron in the intracellular low molecular weight pool, which catalyzes the Fenton reactions during reperfusion after ischemia. In this study a new approach is presented to measure low molecular weight iron and it is applied to normal hearts during ischemia and to iron-loaded hearts during anoxia and reoxygenation. The results of this study show that (a) during ischemia in normal hearts a progressive 30-fold increase occurs in low molecular weight iron after 45 min of ischemia, whereas (b) during 45 min of anoxic perfusion the low molecular weight iron does not increase. This means that the reductive release from the storage protein ferritin is greatly enhanced by the acidification that occurs during ischemia. (c) Anoxic perfusion of iron-loaded hearts does increase low molecular weight iron and there is a further increase upon reoxygenation, which is prevented by (+ )-cyanidanol-3. Based on these findings it is concluded that oxygen deprivation enhances the susceptibility of rat hearts to oxygen radicals by increasing the amount of catalytic, ferrous iron in the low molecular weight pool. (J. Clin. Invest.

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Section: Discussionmentioning

confidence: 59%

Section: Experimental Designmentioning

confidence: 66%

Section: Discussionmentioning

confidence: 89%

Section: Low Molecular Weight Iron Measurementsmentioning

confidence: 99%

See 2 more Smart Citations

Low molecular weight iron and the oxygen paradox in isolated rat hearts.

Voogd¹,

Sluiter²,

Eijk³

et al. 1992

J. Clin. Invest.

111

View full text Add to dashboard Cite

show abstract

“…Hydrophobic Fe complexes are known to exist as many siderophores possess a hydrophobic tail which facilitates the uptake of iron by the phytoplankton (Martinez et al, 2000). A number of siderophore complexes are quantitatively retained by C18 columns including the terrestrial siderophore desferrioxamine B and its Fe chelate, ferrioxiamine B (Gower et al, 1989). This has lead to the development of extraction techniques for siderophores from seawater using C18 solid phase extraction (Freeman and Boyer, 1992).…”

Section: Hydrophobic Organic Fe Complexesmentioning

confidence: 99%

Utilizing Radioisotopes for Trace Metal Speciation Measurements in Seawater

Croot¹,

Heller²,

Schlösser³

et al. 2011

Radioisotopes - Applications in Physical Sciences

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Sampling of low molecular weight iron by microdialysis following spinal cord injury

Castro

Alcock

McAdoo

1999

J of Neuroscience Research

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We combined the use of desferrioxamine as an iron chelator, microdialysis sampling, and iron analysis by atomic absorption spectroscopy to measure extracellular levels of low molecular weight (LMW) iron in vivo in the spinal cord. Low molecular weight iron is free iron plus iron bound to small molecules. We show that the extracellular LMW-iron concentration is not increased significantly in situ by trauma to the rat spinal cord, suggesting that an extracellular elevation in LMW-iron at the site of injury is not the major initiating factor for lipid peroxidation following spinal cord injury.

show abstract

Determination of desferrioxamine-available iron in biological tissues by high-pressure liquid chromatography

Cited by 40 publications

References 19 publications

Low molecular weight iron and the oxygen paradox in isolated rat hearts.

Low molecular weight iron and the oxygen paradox in isolated rat hearts.

Utilizing Radioisotopes for Trace Metal Speciation Measurements in Seawater

Sampling of low molecular weight iron by microdialysis following spinal cord injury

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