Determination of Density of Starch Hydrogel Microspheres from Sedimentation Experiments Using Non-Stokes Drag Coefficient

Abstract

Sedimentation is an important property of colloidal systems that should be considered when designing pharmaceutical formulations. In pharmaceutical applications, sedimentation is normally described using Stokes’ law, which assumes laminar flow of fluid. In this work we studied swelling and hydration of spherical cross-linked amorphous starch microspheres in pure water, solutions of sodium chloride, and in pH-adjusted aqueous solutions. We demonstrated that Reynolds numbers obtained in these experiments correspond to the transition regime between the laminar flow and the turbulent flow and, hence, expressions based on the non-Stokes drag coefficient should be used for calculations of sedimentation velocity from known density or for assessment of density from observed sedimentation velocity. The density of starch microparticles hydrated in water was about 1050 kg/m3, while densities obtained from experiment with other liquids were dependent on the liquids’ densities. The data indicate that the swelling of the cross-linked starch microparticles as characterized by their densities is not sensitive to pH and salt concentration in the studied range of these parameters.

A Structural Study on Absorption of Lysozyme in Amorphous Starch Microspheres

“…However, a recent study showed that the DSM density and degree of swelling undergo only minor changes upon variation of the aqueous solution properties. 41 Hence, the observed DCL change can be explained by the lysozyme forcing the chains closer together to make space for the protein at higher concentrations. Considering that the DSM particles are ≈400 μm in diameter, there could be changes in the particles on distances too large for SAXS; however, the particles look very similar in the microscope whether in the presence or absence of lysozyme ( Figure S5 ).…”

“…The observed correlation length change could potentially be explained by the effect of the ionic strength and pH that addition of polymer may cause. However, a recent study showed that the DSM density and degree of swelling undergo only minor changes upon variation of the aqueous solution properties . Hence, the observed DCL change can be explained by the lysozyme forcing the chains closer together to make space for the protein at higher concentrations.…”