Determination of DDT by Bioassay

Pagan, C; Hageman, R. H.

doi:10.1126/science.112.2904.222

Cited by 3 publications

(3 citation statements)

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“…Woke (705), Simmons (87), and Nolan and Wilcoxon (77) were among the first to use mosquito larvae for the determination of insecticide residues. Although Aedes agypti (L.) (73)(74)(75)(76)(77) have been used most frequently, A. vexans, A. sticticus (Meig.) (74), Anopheles quadramaculatus Say (77), Culex quinquefasciatus Say (78,93,94), and C. fatigens Wied.…”

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confidence: 99%

“…Mosquitoes are more difficult to handle and rear than houseflies, as most of them require a blood meal for egg production. They are subject to considerable variation unless special precautions are observed (75,43) and are sensitive to natural constituents extracted from plant or animal tissue (90). Although their extreme sensitivity is usually an advantage, they are susceptible to contamination and special care may be necessary to prevent loss of the colony.…”

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confidence: 99%

“…been used byPagan and Hageman(75) for detecting small quantities of DDT added to beans and tomatoes.…”

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Pesticide Residues, Utility of Bioassay in the Determination of Pesticide Residues

Dewey¹

1958

J. Agric. Food Chem.

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A review of bioassay techniques for the quantitative determination of pesticide residues has been made. Several organisms are in use. Selection depends on the pesticide used, reproducibility, indication of the true quantity of toxicant present, cost, speed of assay, and other practical limitations. The types of exposure, responses measured, sensitivity, and complexity vary with different methods using the same organism and with different organisms. Though possibly less specific than chemical methods, bioassay offers the opportunity to determine the presence of toxic metabolites which may be bypassed by more specific methods. Bioassay, in its broadest sense, may refer to any method in which some property of a material is measured in terms of a biological response. In the agricultural pesticides field, practically all chemical or biological control programs involving response of an organism to a chemical may be called bioassays. Hoskins (57) has divided bioassay into three groups: screening and formulation, resistance, and residue analysis. Bioassay will be defined here as the quantitative determination of a toxicant using biological material to measure

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