Determination of dapsone in serum and saliva using reversed-phase high-performance liquid chromatography with ultraviolet or electrochemical detection

Moncrieff, Joan

doi:10.1016/0378-4347(93)e0439-w

Cited by 15 publications

(4 citation statements)

References 21 publications

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“…The in-vitro drug concentrations employed in this study were more than one order of magnitude greater than dapsone plasma concentrations previously reported in dermatitis herpetiformis patients (up to 4 0 m~; Harvath et a1 1986;Moncrieff 1994). Previous studies have, however, observed a discrepancy between in-vitro inhibitory concentrations of dapsone and its actual therapeutic effect.…”

Section: Discussioncontrasting

confidence: 49%

Studies on the Inhibitory Effects of Analogues of Dapsone on Neutrophil Function In-vitro

Coleman

Smith

Perris

et al. 1997

Journal of Pharmacy and Pharmacology

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We have compared twelve sulphone analogues of dapsone in terms of inhibition both of zymosan-mediated human neutrophil respiratory burst and inhibition of interleukin-1-stimulated neutrophil adhesion to transformed human umbilical vein endothelial cells. Overall, there was a good correlation between the respective rank orders of compound potency in the two test systems. The most effective compounds in terms of respiratory burst and adherence inhibition were the 2-nitro-4-amino-, 2-hydroxy-4-aminopropyl-, and 2-methoxy-4-aminoethyl- derivatives. In general, potency was inversely associated with lipophilicity; compounds with bulky side-chains, e.g. the 2-methyl-4-aminopentyl, 2-methyl-4-aminohexyl and the 2-hydroxymethyl-4-aminoethyl derivatives, were less potent. A 2-hydroxy-4-amino- derivative was the exception, however, with low lipophilicity and relatively low potency. All of the compounds tested showed comparable or greater inhibition in both the neutrophil-mediated assays compared with dapsone. Some of the compounds might, because of their good tissue penetration and lower toxicity than dapsone, have the potential to undergo further development.

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Section: Discussioncontrasting

confidence: 49%

Studies on the Inhibitory Effects of Analogues of Dapsone on Neutrophil Function In-vitro

Coleman

Smith

Perris

et al. 1997

Journal of Pharmacy and Pharmacology

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“…It has been studied extensively for pharmaceutical research such as bioavailability [4], biotransformation [5], and formulations, [6]. It has been detected in plasma, urine and saliva [7,8] using different analytical methods such as liquid chromatography with, UV-Visible [8], fluorescence [9] and mass spectrometry [10], as well as electrochemical detection [8]. However, no study was found for the detection of the Dapsone drug in wastewater.…”

Section: Introductionmentioning

confidence: 99%

An Undergraduate Experiment Using Cyclodextrin – Assisted Sensitive Fluorescence Detection and Quantitation of Dapsone Drug in Wastewater Samples

Meetani¹,

Alhalabi²,

Al-tabaji³

et al. 2019

WJCE

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The guest-host interaction between Dapsone drug and β-cyclodextrin (β-CD) was investigated using fluorescence spectroscopy, 1 H-NMR, and liquid chromatography with fluorescence detection. The optimized conditions for the interaction were investigated by spectrofluorometry and were found to be at 0.2 mg/mL (0.176 mM) of β-CD and pH 8.8. For these conditions, very low concentration of Dapsone drug of 2.4 ng/mL (12.97 nM) can be detected. The standard addition method was utilized to detect Dapsone in influent and effluent wastewater samples in the sub parts per billion concentration range by HPLC-FLD using β-CD as an additive in the mobile phase.

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“…The HPLC methods for the determination of DDS and its metabolite, MADDS, in plasma, whole blood, [8,9] serum, and saliva, [10] and LC-MS= MS methods for the determination of DDS in muscle, [11] meat, and milk [12] have been reported. Determination of CPG and its metabolite, CCG, in human plasma, erythrocyte and urine [13,14] using HPLC has also been performed.…”

Section: Introductionmentioning

confidence: 99%

Sensitive and Specific Lc-MS/MS Method for the Simultaneous Determination of Chlorproguanil, Dapsone, and Their Metabolites in Human Plasma

Sethi

Dua

Jain

2012

Journal of Liquid Chromatography & Related Technologies

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& A sensitive, specific, and rapid liquid chromatography tandem mass spectrometry (HPLC-MS= MS) method for the determination of dapsone, chlorproguanil, and their metabolites was developed and validated over a concentration range of 2-2000 ng=mL using 200 lL of plasma. After a simple solvent precipitation procedure, the supernatant was analyzed directly by HPLC-MS=MS method using an XTerra RP18 (2.1 mm Â 100 mm, 5.0 lm) column with mobile phase consisting of acetonitrile-0.1% formic acid in water (75:25, v=v, pH 3.0) in an isocratic mode at a flow rate of 0.3 mL=min. Mass detection was performed using a triple quadrupole mass spectrometer operating in positive electrospray ionization mode. The elution of chlorproguanil (CPG, 288 ->204), chlorcycloguanil (CCG, 286 ->229), dapsone (DDS, 249 ->156), monoacetyldapsone (MADDS, 291 ->156), and trimipramine-D 3 (TMP-D 3 , 298 ->103) was monitored using multiple reaction monitoring. The lower limit of detection for CPG, CCG, DDS, and MADDS was 0.5 ng mL À1 while the limit of quantification was 2 ng mL À1 in human plasma. The extraction recovery of CPG, CCG, DDS, MADDS, and TMP-D 3 from human plasma was higher than 87%. The method may find its application in therapeutic monitoring of these compounds in biological matrix.

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Determination of dapsone in serum and saliva using reversed-phase high-performance liquid chromatography with ultraviolet or electrochemical detection

Cited by 15 publications

References 21 publications

Studies on the Inhibitory Effects of Analogues of Dapsone on Neutrophil Function In-vitro

Studies on the Inhibitory Effects of Analogues of Dapsone on Neutrophil Function In-vitro

An Undergraduate Experiment Using Cyclodextrin – Assisted Sensitive Fluorescence Detection and Quantitation of Dapsone Drug in Wastewater Samples

Sensitive and Specific Lc-MS/MS Method for the Simultaneous Determination of Chlorproguanil, Dapsone, and Their Metabolites in Human Plasma

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