Determination of d-lactate by enzymatic methods in biological fluids: study of interferences

Abstract

Analysis of nondeproteinized samples with an enzymatic method to determine d-lactate indicated interferences. The presence of l-lactate dehydrogenase (LD) and l-lactate in the sample led to underestimation of d-lactate content when a sample blank was processed and overestimation when it was omitted. We proved that this interference is not due to lack of d-LD stereospecificity. Moreover, assessment of d-LD and l-LD KM for NAD+ allowed us to rule out the different affinities for this coenzyme as a cause of the interference. Our results underline the importance of deproteinizing samples for d-lactate analysis when enzymatic methods are used. The ultrafiltration procedure we propose is convenient and shows acceptable mean recovery (108%) and good imprecision (within-run CV = 4.2% and 3.0% for d-lactate at 31 and 107 μmol/L, respectively; between-run CVs were 7.3% at 49 μmol/L d-lactate and 3.1% at 115 μmol/L d-lactate).

Peritoneal and Plasma d‐lactate Concentrations in Horses with Colic

“…Several methods have been reported to measure d ‐lactate concentrations in body fluids . The d ‐lactic acid spectrophotometer assay is the most widely used and should be easily adaptable for use in most clinical laboratories as it is simple to perform and only requires a d ‐lactate assay kit and a spectrophotometer.…”

“…A commercial d ‐lactate analysis kit (Megazyme International, Wicklow, Ireland) was used according to the manufacturer's recommendations . All calculations were made automatically by the analyzer's software (Mega‐Calc.…”

Serum D‐lactate concentrations in dogs with parvoviral enteritis

“…The use of colorimetric assays however is commonplace in human medicine and in other studies of mammals with results that are comparable to HPLC measurements. 18,[29][30][31][32][33] A recent study using the modified strong ion model in CPV dogs confirmed that acid-base disturbances in this population are multifactorial, with chloride playing an important role in acid-base changes. 34 Our study identified intermittent correlation of D-lactate to multiple acidbase markers, most notably at admission, but no consistent trends were appreciated throughout hospitalization.…”

Are serum amyloid A or D‐lactate useful to diagnose synovial contamination or sepsis in horses?

“… 15 SF and blood samples defrosted at room temperature and then centrifuged using polyethersulfone ultrafiltration membranes (Fisher Scientific, Leicester, UK) at 4000 g for 10 minutes to improve test accuracy. 23 A value of 0 was used for measurements below the limit of detection (0.21 µg/ml).…”