Determination of Cystine by Its Catalytic Effect on Iodine-Azide Reaction

Strickland, Richard D.; Mack, P. A.; Childs, W. A.

doi:10.1021/ac60159a037

Cited by 29 publications

(10 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The method of Strickland, Mack, and Childs (1960) for cystine was modified for the urinary sulphur metabolites of CS2. The iodine-azide reagent was prepared from stock 2M sodium azide and from stock K13, containing 0 065 M 12 and 0 130 M KI.…”

Section: Methodsmentioning

confidence: 99%

Relevancy of bivalent sulphur excretion to carbon disulphide exposure in different metabolic conditions

Magós

1972

Occupational and Environmental Medicine

View full text Add to dashboard Cite

Magos, L. (1972). Brit. J. industr. Med., 29,[90][91][92][93][94]. Relevancy of bivalent sulphur excretion to carbon disulphide exposure in different metabolic conditions. A quantitative spectrophotometric method for the estimation of urinary bivalent sulphur by its catalytic effect on the iodine-azide reaction has been developed for rats exposed to carbon disulphide (CS2). Urinary CS2 was also determined. Exposure to CS2 increased the excretion of bivalent sulphur in both fed and starved animals, but the increase in starved animals was significantly less than in fed animals. Starvation also decreased the urinary excretion of CS2. Phenobarbitone pretreatment, which induced liver damage in animals subsequently exposed to CS2, had no effect on either bivalent sulphur or CS2 excretion. Three dithiocarbamate fungicides, Thiram, Ziram, and Zineb, given by mouth increased the excretion of bivalent sulphur in the following order: Zineb < Ziram < Thiram.After 250 mg/kg sodium diethyldithiocarbamate (DDC) given intraperitoneally the same amount of CS2 was excreted in the urine as after 4 hours' exposure to 2-0 mg/l CS2, but the bivalent sulphur excretion was six times higher. Starvation (Strbmme, 1965). After inhalation of CS2 8 to 30% of the body burden is exhaled and less than 1 % is excreted in the urine of persons exposed to CS2 (Teisinger and Soucek, 1949;Harashima and Masuda, 1962 McKee, 1950;Stromme, 1965). The second group of major metabolites constitutes compounds which retained sulphur in the bivalent form. Their excretion is the best indicator of the level of CS2 exposure when assayed by using their catalytic effect on the reduction of iodine by azide (Vasak,

show abstract

Section: Methodsmentioning

confidence: 99%

Relevancy of bivalent sulphur excretion to carbon disulphide exposure in different metabolic conditions

Magós

1972

Occupational and Environmental Medicine

View full text Add to dashboard Cite

show abstract

“…Among organic compounds, thiols, thiones, disulfdes, organophosphorus compounds containing P¼S and P-S bonds, and compounds with sulfur in a ring (after cleavage with iodide azide) have induction properties. The mechanism of the iodine-azide reaction was introduced by Strickland, Mack, and Childs (1960) and modified by Kurzawa and Kurzawa (1986). The iodine-azide reaction takes places if sulfur(II) is present in the solution; sulfur plays a key role as the inductor.…”

Section: Introductionmentioning

confidence: 99%

Postcolumn Iodine–Azide Reaction as a Detection System in HPLC for the Determination of Methylthiouracil in Urine

Zakrzewski

2009

Analytical Letters

View full text Add to dashboard Cite

This article describes a system for determining methylthiouracil in urine based on the sensitizing induction of methylthiouracil on the reaction between iodine and azide ions and combined techniques of high-performance liquid chromatography and simple postcolumn detection. The optimal conditions for iodineazide reaction and high-performance liquid chromatographic separation were determined. The reproducibility, linearity, and recovery were evaluated under the optimal conditions. The methylthiouracil standards added to normal urine show that the response of the detector, set at 350 nm (corresponding to unreacted iodine in the postcolumn iodine-azide reaction), is linear within the concentration range of 0.4-5.0 nmol mL À1 urine. The relative standard deviation values for precision and recovery within the calibration range varied from 0.9 to 4.8% and from 94 to 105%, respectively. Limits of detection (LOD) and quantitation (LOQ) were 0.3 and 0.4 nmol mL À1 urine, respectively.

show abstract

“…Both the samples showed identical features (Scheme 1). 26 Radioactive nucleotides were identified by autoradiography. A Shimadzu HPLC was used with a UV-Visible detector of LDC Milton Roy make.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of deoxyadenosine‐5′‐(³⁵S)‐thiomonophosphate [dAMP(³⁵S)] of high specific activity – a key intermediate for the synthesis of Sp‐deoxyadenosine‐5′‐(alpha;‐³⁵S)‐thiotriphosphate [Sp‐dATP (α‐³⁵S)]

Mathew

Ravi

Sontakke

et al. 2001

Labelled Comp Radiopharmac

View full text Add to dashboard Cite

Determination of Cystine by Its Catalytic Effect on Iodine-Azide Reaction

Cited by 29 publications

References 11 publications

Relevancy of bivalent sulphur excretion to carbon disulphide exposure in different metabolic conditions

Relevancy of bivalent sulphur excretion to carbon disulphide exposure in different metabolic conditions

Postcolumn Iodine–Azide Reaction as a Detection System in HPLC for the Determination of Methylthiouracil in Urine

Synthesis of deoxyadenosine‐5′‐(³⁵S)‐thiomonophosphate [dAMP(³⁵S)] of high specific activity – a key intermediate for the synthesis of Sp‐deoxyadenosine‐5′‐(alpha;‐³⁵S)‐thiotriphosphate [Sp‐dATP (α‐³⁵S)]

Contact Info

Product

Resources

About

Determination of Cystine by Its Catalytic Effect on Iodine-Azide Reaction

Cited by 29 publications

References 11 publications

Relevancy of bivalent sulphur excretion to carbon disulphide exposure in different metabolic conditions

Relevancy of bivalent sulphur excretion to carbon disulphide exposure in different metabolic conditions

Postcolumn Iodine–Azide Reaction as a Detection System in HPLC for the Determination of Methylthiouracil in Urine

Synthesis of deoxyadenosine‐5′‐(35S)‐thiomonophosphate [dAMP(35S)] of high specific activity – a key intermediate for the synthesis of Sp‐deoxyadenosine‐5′‐(alpha;‐35S)‐thiotriphosphate [Sp‐dATP (α‐35S)]

Contact Info

Product

Resources

About

Synthesis of deoxyadenosine‐5′‐(³⁵S)‐thiomonophosphate [dAMP(³⁵S)] of high specific activity – a key intermediate for the synthesis of Sp‐deoxyadenosine‐5′‐(alpha;‐³⁵S)‐thiotriphosphate [Sp‐dATP (α‐³⁵S)]